Trends in Phytochemical Research: 1 (1)

Open Access Article

1 - Exploration of metabolic variations, anti-cholinesterase, anti-heme biocrystallization, and anti-protein denaturation activities of ten Capsicum accessions under different stages of ripening
Mamita Debnath Jhelam Chatterjee Susmita Das

Hydro-methanolic extracts of pericarps and seeds of 10 different Capsicum cultivars from three different species viz., Capsicum chinense, C. annuum and C. frutescens were assessed to determine metabolic variations and anti-cholinesterase, anti-malarial and anti-protein More

Hydro-methanolic extracts of pericarps and seeds of 10 different Capsicum cultivars from three different species viz., Capsicum chinense, C. annuum and C. frutescens were assessed to determine metabolic variations and anti-cholinesterase, anti-malarial and anti-protein denaturation activities at four different stages of ripening, namely green unripe (GU), turning green-orange (TGO), orange ripe (OR) and red fully ripe (RFR) stages. Many of the ripening stages of the tested cultivars showed significant bioactivities in a dose-dependent manner. In addition, metabolites were identified using GC/MS. On the other hand, HPLC analysis revealed the highest capsaicin and dihydrocapsaicin content in the pericarp of GU, TGO and RFR stages of C. chinense. β-Carotene, capsanthin and lutein contents of C. chinense were also measured using HPTLC technique. The GU and RFR stages of C. chinense contained appropriate amounts of all three carotenoids. These results may be helpful in selecting the cultivars with the best attributes. Manuscript profile

Open Access Article

2 - Anti-inflammatory and analgesic effects of aqueous extracts of Vitex agnus cactus L. and Cymbopogon nardus L. against carrageenan-induced inflammation in rats
Fatima El Kamari Driss Ousaaid Laila Lahrizi Abdelfettah El Moussaoui Badiaa Lyoussi

Vitex agnus cactus and Cymbopogon nardus are widely used in traditional and conventional medicine as natural anti-inflammatory agents. Within this framework, the current study was undertaken to examine in vivo the anti-inflammatory and analgesic effects of aqueous extra More

Vitex agnus cactus and Cymbopogon nardus are widely used in traditional and conventional medicine as natural anti-inflammatory agents. Within this framework, the current study was undertaken to examine in vivo the anti-inflammatory and analgesic effects of aqueous extracts of the leaves and seeds of V. agnus cactus and C. nardus. In this relation, aqueous extracts were prepared from the leaves and fruits of V. agnus cactus and the leaves of C. nardus. The inflammatory process was induced using the carrageenan method. The analysis of the obtained results revealed that the aqueous extract of leaves of V. agnus cactus exhibited the highest antioxidant content (80.22 ± 11.7 mg GAE/g for TPC, 72.14 ± 9 mg RE/g for TFC, 680 ± 19.6 mg QE/g for flavones and flavonols, 355.33 ± 23.36 mg AAE/g for TAC, 0.33 ± 0.04 mg/mL for IC50-DPPH, and 0.97 ± 0.04 mg/mL for EC50-FRAP) and anti-inflammatory effect with dose-dependent manner. These results suggest that these plants have the potential to alleviate pain and inflammation when used for therapeutic purposes. Manuscript profile

Open Access Article

3 - Simultaneous optimization of extraction of bioactive compounds and antioxidant activity of Ammi visnaga (L.) Lam aerial parts using response surface methodology
Zineb El Jabboury Smail Aazza Driss Ousaaid Oumaima Chater Uroš Gašić Peđa Janaćković Zora Dajic Stevanovic Stefan Kolašinac Meryem Benjelloun Lahsen El Ghadraoui

In this report, different extracts from the aerial parts of Ammi visnaga (L.), e.g., flowers, leaves, and stems were prepared using water, methanol, and ethanol. To optimize the extraction process, the design of mixtures was carried out using different extracting solven More

In this report, different extracts from the aerial parts of Ammi visnaga (L.), e.g., flowers, leaves, and stems were prepared using water, methanol, and ethanol. To optimize the extraction process, the design of mixtures was carried out using different extracting solvents and their combinations. The special cubic model explained the variance of the TPC and the antioxidant activity of the extracts at a level of R2 > 95%. In general, the analysis of the model-derived response surfaces revealed that in binary mixtures (50% ethanol + 50% methanol), the yielded values of phenolic compounds and the antioxidant activity increase with the water proportion of different prepared mixtures. The ability of the quaternary mixture to extract the phenolic compounds was also positively and significantly influenced by the water content, creating a mild polar medium for the extraction of phenolic compounds. The phenolic profile of different extracts under study revealed the presence of a cocktail of active ingredients, including chlorogenic acid, caffeic acid, rutin, p-coumaric acid, etc. especially the flower extract of A. visnaga (L.). Manuscript profile

Open Access Article

4 - A comparative study on the extracts from the fruits of Ficus auriculata L.: GC-MS profiling, phytochemical composition, biological activities and in-silico ADMET study
Garima Tamta Nisha Mehra Shishir Tandon Viveka Nand Manish Pant Vinita Gouri

Ficus auriculata L., commonly known as, “elephant ear fig” is a species of fig tree in Moraceae family and globally found in tropical and subtropical forests. The present comparative study investigated the GC-MS analysis, phytochemical composition, in vitro antioxidant More

Ficus auriculata L., commonly known as, “elephant ear fig” is a species of fig tree in Moraceae family and globally found in tropical and subtropical forests. The present comparative study investigated the GC-MS analysis, phytochemical composition, in vitro antioxidant assays and antidiabetic activity of methanol and hexane extracts from the fruits of Ficus auriculata which was collected from two different agro-climatic conditions in Uttarakhand, namely Almora (Hill region) and Haldwani (Tarai region). The GC-MS analysis of Almora unripe hexane fruit extract (AUFHE) and Haldwani unripe hexane fruit extract (HUFHE) gave rise to the characterization of two chemical profiles composed of 37 and 40 bioactive compounds with γ-sitosterol (15.46% and13.44%) as the most abundant component, respectively. Moreover, in Almora unripe methanol fruit extract (AUFME) and Haldwani unripe methanol fruit extract (HUFME), 24 and 23 bioactive compounds were characterized among which linoleic acid (71.41%) and hexadecadienoate (26.42%) were the most prevalent compounds, respectively. In view of the obtained results, HUFME exhibited prominent total phenolic, flavonoid and tannin contents. AUFME also showed potent antioxidant activity when using DPPH (2,2-diphenylpicrylhydrazyl) radical scavenging activity assay (IC50 =447.45 ± 0.53 µg/mL), whereas strong metal chelation assay was found for HUFHE (IC50= 502.07 ± 2.50 µg/mL). Furthermore, AUFME and HUFME displayed potent anti-diabetic activity. In addition, ADMET study predicted that F. auriculata could be considered an effective bioactive source of phytoconstituents for various biological efficacies. The observed pharmacological properties could be attributed to the presence of polyphenols, flavonoids and fatty acids in F. auriculata fruit. Manuscript profile

Open Access Article

5 - Phytochemical analysis of trunk bark with branch bark, wood and stored bark of Ficus racemosa and comparison with other Ficus barks
Manikandan Velanthavalam Logeswaran Vellingiri Vadivel

The phytochemistry of F. racemosa bark was compared with other Ficus species available in South India (F. religiosa, F. benghalensis and F. hispida barks). Further, fresh bark was compared with stored bark of F. racemosa; wood with the trunk bark of F. racemosa; branch More

The phytochemistry of F. racemosa bark was compared with other Ficus species available in South India (F. religiosa, F. benghalensis and F. hispida barks). Further, fresh bark was compared with stored bark of F. racemosa; wood with the trunk bark of F. racemosa; branch bark with the trunk bark of F. racemosa. Also the protective potential of active fraction was evaluated against oxidative stress in ex vivo skin model. Qualitative and quantitative estimation of phytochemicals were done for Ficus species bark samples and also subjected to UPLC-QTOF-MS analysis. It was found that methanolic extract of F. racemosa has a high number of phytochemicals (Flavonoids: 4.10 mg/mL; phytosterols: 3.28 mg/mL; total phenols: 29.93 mg/mL and tannins: 8.98 mg/mL). Kaempferol-3-O-β-D-glucoside was noted as the major compound in F. racemosa bark. Trunk bark of F. racemosa was quantified to have high amount of phytochemicals than branch bark. Also, bark was found to contain higher concentration of different phytochemicals than F. racemosa wood. Stored bark of F. racemosa was found to exhibit lesser amount of phytochemicals than fresh bark. F. racemosa bark also exhibits moderate superoxide and hydrogen peroxide scavenging activities, high inhibition of lipid peroxidation and moderate inhibition of protein oxidation. Manuscript profile

Open Access Article

6 - Blood coagulation effect of combined extract of Thymus vulgaris and Medicago sativa
Zahra Sadat Mashkani Jafar Vatandoost Toktam Hajjar Behnam Mahdavi

Thymus vulgaris and Mediacgo sativa, are used as a traditional remedy in the treatment of bleeding disorders. Considering their probabilistic coagulation compounds, in an animal study, forty male mice were randomly divided into 5 groups (n = 8) as well as negative and More

Thymus vulgaris and Mediacgo sativa, are used as a traditional remedy in the treatment of bleeding disorders. Considering their probabilistic coagulation compounds, in an animal study, forty male mice were randomly divided into 5 groups (n = 8) as well as negative and positive control. Coagulation indices include bleeding time (BT), clotting time (CT), and the number of platelets (PLT) were examined on the 13th day of treatment. A significant reduction in the BT and CT tests, as well as a significant increase in PLT in the treated groups was observed. It is concluded that although the T. vulgaris and M. sativa extracts have a coagulation effect through primary homeostasis and a common pathway of secondary hemostasis, combined extracts are more effective than individual extracts. Moreover, phenolic and flavonoid compounds are the most affecting compounds that affect platelet number and aggregation. Manuscript profile

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