The frequency of gastrointestinal ulceration is higher in cholestatic than in healthypopulation.Melatonin is powerful antioxidant, that does not undergo redox cycling.Vitamin C isan antioxidant,that undergo redox cycling. Cysteamine is a most potent agent for inducingga More
The frequency of gastrointestinal ulceration is higher in cholestatic than in healthypopulation.Melatonin is powerful antioxidant, that does not undergo redox cycling.Vitamin C isan antioxidant,that undergo redox cycling. Cysteamine is a most potent agent for inducinggastrointestinal ulcer and its ulcerogenic effect may be due to generation of ROS, and increasingduodenal endothelin-1 concentration.The aim of this study was to evaluate the effect of pretreatment with melatonin on cysteamineinducedgastric ulcer in unoperated control (UOC), and bile duct-ligated (BDL) rats.This study have performed on 2 groups of rats: UOC and BDL. each group was divided into 4subgroups.these subgroups were treated with saline, cysteamine, vitamin C plus cysteamine andmelatonin plus cysteamine respectively.All rats were killed 24h after the last injection andstomach was prepared for calculation of j.score. In BDL group, the common bile duct wasdoubly ligated and after7 days, rats had shown overt jaundice. BDL group was treated like theUOC group .In UOC group, injection of cysteamine was associated with significant increased in j.scorecompared with saline group. injection of vitamin C and melatonin, was associated with decreasein and j.score compared with cysteamine group. In BDL group injected with cysteamine, j.scorewere significantly more sever compared with saline group. injection of vitamin C and melatoninwas associated with significant decrease in j.score compared with cysteamine group.Our results suggest that pretreatment with melatonin , protect UOC and BDL rats againstcysteamine-induced gastric ulcer possibly by ability to improve oxidative stress.
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This study was aimed at determining the effect of cysteamine supplement during in vitromaturation of bovine oocytes. Cumulus-oocyte complexes (COCs) from abattoir ovaries werewere obtained from local abattoir (Tabriz abattoir, East Azarbaijan, Iran) shortly after slaugh More
This study was aimed at determining the effect of cysteamine supplement during in vitromaturation of bovine oocytes. Cumulus-oocyte complexes (COCs) from abattoir ovaries werewere obtained from local abattoir (Tabriz abattoir, East Azarbaijan, Iran) shortly after slaughterand transported to the laboratory in 0.9% NaCl solution plus 100 IU/ml potassium penicillin G,and 100 μg/ml streptomycin sulfate at 30-35°C, within 2 to 4 h from slaughter. Cumulus-oocytecomplexes (COCs) from abattoir ovaries were matured in vitro in Hepes-TCM 199supplemented with 0.2 mM sodium pyruvate, 1 μg/ml 17-β-estradiol, 10% fetal calf serum(FCS), 0.5 μg/ml bFSH and 0 (control) and 0.1, 0.5 or 1 mM/ml of cysteamine for 24 h. WhenCOCs matured in TCM 199 media with 0.1 mM/ml cysteamine, the rate of maturation wereincreased as compared with control group (46.66 vs 33.33%, respectively). The maturation rateof oocytes in media with 0.5 mM/ml cysteamine (56.55%) was significantly higher than controlgroup (33.33%) (P<0.05). Also, the percentage of unmatured oocytes in 1 mM/ml treatmentgroup lower than other groups (P<0.05). The results demonstrate that cysteamine when presentduring IVM, improved bovine oocyte maturation.
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This study was designed to investigate the effect of cysteamine and vitamin E on post thaw buffalo bull's sperm quality. For this purpose, 20 ejaculates from four buffalo bulls possessing more than 70% visual sperm motility were diluted at 37◦C in BioXcell& More
This study was designed to investigate the effect of cysteamine and vitamin E on post thaw buffalo bull's sperm quality. For this purpose, 20 ejaculates from four buffalo bulls possessing more than 70% visual sperm motility were diluted at 37◦C in BioXcell® extender. The diluted semen was cooled to 4◦C within 2 hours, equilibrated at 4◦C following the addition of (0.75, 1.5, 2 and 5 mM) of vitamin E and (7.5, 12.5, 15 and 20 mM) cysteamine per 90 ml, filled in 0.5 ml French straws and were subjected to cooling condition before being plunged into liquid nitrogen. Semen was thawed at 37◦C for 40 seconds after 72 hours of storage inside liquid nitrogen .Post-thaw sperm motility and some qualitative parameters of each frozen semen sample were assessed by using computer assisted semen analyzer (CASA). In general, the results showed that the addition of 1.5 mM vitamin E and 20 mM cysteamine in the commercial diluents BioXcell extender for freezing buffalo semen increased the motility of spermatozoa and some qualitative parameters to post-thawed buffalo sperm.
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Benefts of antioxidant supplementation in various disorders through reducing free radicals and improving organsperformance have been reported. The aim of this study was to investigate the effects of coenzyme Q10 and vitaminC on Cysteamine-induced lipid peroxidation and More
Benefts of antioxidant supplementation in various disorders through reducing free radicals and improving organsperformance have been reported. The aim of this study was to investigate the effects of coenzyme Q10 and vitaminC on Cysteamine-induced lipid peroxidation and oxidative stress.This experimental interventional study was conducted on male Wistar rats. Animals were divided into four groups(six rats) randomly. Groups were treated as; group 1 (Normal saline), Group 2 (Cysteamine), Group 3 (vitaminC plus Cysteamine), Group 4 (coenzyme Q10 plus Cysteamine). 24 hours after the last injection, rats wereanesthetized and sampled for investigations. Welch’s and Kruskal-Wallis tests were used for analyzing data and P< 0.05 was set the signifcance level.The results of this study indicate that injection of cysteamine signifcantly (P < 0.05) decreased glutathioneperoxidase activity compared with control group. Pretreatment with vitamin C signifcantly (P < 0.05) increasedglutathione peroxidase activity compared with cysteamine group. Pretreatment with coenzyme Q10 increasedglutathione peroxidase activity (P <0.001) and superoxide dismutase (P <0.05) signifcantly compared withcysteamine group.Based on the results of this study, coenzyme Q10 and vitamin C can be used in reducing oxidative stress inducedcysteamine.
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