Effects of cysteamine and vitamin E addition to semen extender on post-thawed microscopic sperm parameters of buffalo bulls

Subject Areas : Veterinary Clinical Pathology

rahim beheshti ¹ , jamshid ghiyasi ²

1 - دانشگاه آزاد اسلامی، واحد شبستر، استادیار گروه علوم درمانگاهی، شبستر، ایران.
2 - دانشگاه آزاد اسلامی، واحد شبستر، استادیار گروه علوم پایه، شبستر، ایران.

Received: 2013-11-03 Accepted : 2014-06-24 Published : 2014-02-20

Keywords: vitamin E, Buffalo, Semen freezing, Cysteamine,

Abstract :

   This study was designed to investigate the effect of cysteamine and vitamin E on post thaw buffalo bull's sperm quality. For this purpose, 20 ejaculates from four buffalo bulls possessing more than 70% visual sperm motility were diluted at 37◦C in BioXcell® extender. The diluted semen was cooled to 4◦C within 2 hours, equilibrated at 4◦C following the addition of (0.75, 1.5, 2 and 5 mM) of vitamin E and (7.5, 12.5, 15 and 20 mM) cysteamine per 90 ml, filled in 0.5 ml French straws and were subjected to cooling condition before being plunged into liquid nitrogen. Semen was thawed at 37◦C for 40 seconds after 72 hours of storage inside liquid nitrogen .Post-thaw sperm motility and some qualitative parameters of each frozen semen sample were assessed by using computer assisted semen analyzer (CASA). In general, the results showed that the addition of 1.5 mM vitamin E and 20 mM cysteamine in the commercial diluents BioXcell extender for freezing buffalo semen increased the motility of spermatozoa and some qualitative parameters to post-thawed buffalo sperm.

References:

• منافی آذر، ق.، محسن پور آذری، ع. و رزاق زاده، س. (1389). بررسی وضعیت بهداشت و تولید مثل گاومیش در شرایط بومی استان آذربایجان غربی. مجموعه گزارش­های علمی، مرکز پژوهش­ها منابع طبیعی و امور دام استان  آذربایجان­غربی، قابل دسترسی در تارنمای: http://www.jkmt.ir/article/m2/455.pdf.
  
  
  
  • Ahmad, Z., Anzar, M., Shahab, M., Ahmad, N. and Andrabi, S.M.H. (2003). Sephadex and sephadex ion-exchange filtration improves the quality and freezability of low-grade buffalo semen ejaculates. Theriogenology, 59: 1189-1202.
  
  
  • Andrabi, S.M.H., Ansari, M.S., Ullah, N. and Afzal, M. (2008). Effect of non-enzymatic antioxidants in extender on post-thaw quality of buffalo (bubalus bubalis) bull spermatozoa. Pakistan Veterinary Journal, 28(4): 159-162.
  
  
  • Bansal, A.K. and Bilaspuri, G.S. (2009). Antioxidant effect of vitamin E on motility, viability and lipid peroxidation of cattle spermatozoa under oxidative stress. Animal Science Papers and Reports, 27(1): 5-14.
  
  
  • Breinger, E., Beorlegui, N., Flaherty, C. and Beconi, M. (2005). Alpha-tocopherol improves biochemical and dynamic parameters in cryopreserved boar. Theriogenology, 63: 2126-2135.
  
  
  • Brezezinska, E., Slebodzinski, A., Pietras, B. and Wieczork, G. (1995). Antioxidant effect of vitamin E and glutathione on lipid peroxidation in boar seminal plasma. Biological Trace Element Research, 47: 69-74.
  
  
  • Bucak, M.N., Atessahin, A., Varıslı, O., Yuce, A., Tekin, N. and Akcay, A. (2007). The influence of trehalose, taurine, cysteamine and hyaluronan on ram semen: Microscopic and oxidative stress parameters after the freeze–thawing process. Theriogenology, 67(5): 1060-1067.
  
  
  • Ciftci, H., Verit, A., Savas, M., Yeni, E. and Erel, O. (2009). Effects of N-acetylcysteine on semen parameters and oxidative/antioxidant status. Urology, 74(1): 73-76.
  
  
  • Dehghani Ghale-jughi, H. (1991). Study of reproductive glands in non-castrated male buffaloes in west Azerbaijan. DVM thesis, Urmia University, Thesis No. 245.
  
  
  • El-Sheshtawy, R.I., El-Sisy, G.A. and El-Nattat, W.S. (2008). Use of selected amino acid to improve buffalo bull semen cryopreservation. Global Veterinaria, 2(4): 146-150.    
  
  
  • Food and Agricultural Organization. (2004). FAOSTAT, global livestock production and health atlas. Animal production and health division, Rome, Italy. www.fao.org/ag/aga/glipha/index.jsp.
  
  
  • Kumaresan, A., Ansari, M.R. and Abhishek, G. (2005). Modulation of post-thaw sperm functions with oviductal proteins in buffaloes. Animal Reproduction Science, 90: 73-84.
  
  
  • Lewis, S.E.M., Boyle, P.M., Mckinney, K.A., Young I.S. and Thompson, W. (1997). Comparison of individual antioxidants of sperm and seminal plasma in fertile and infertile men. Fertility and Sterility, 67: 142-147.
  
  
  • Movahedin, M., Bejnurdi, M., Amanpour, S. and Hamid Abadi, H. (2008). Effect of antioxidant on quality of frozen–thawed sperm by vitrification method, Journal of Mazandaran University of Medical Sciences, 63: 27-20.
  
  
  • Oeda, T., Henkel, R., Ohmori, H. and Schill, W.B. (1997). Scavenging effect of N-acetyl-l-cysteine against reactive oxygen species in human semen: a possible therapeutic modality for male factor infertility. Andrologia, 29: 125-131.
  
  
  • Saacke, R.G. (1984). Semen quality: importance of and influencing factors (abstract). Paper. Presented in NAAB 10th Technical Conference on Artificial Insemination and Reproduction, pp: 30-36.
  
  
  • Senatore, E.M., Verberckmoes, S., Pascale, M. and Presicce, GA. (2004). A deep utero-tubal semen deposition in Mediterranean Italian buffaloes using a new artificial insemination device. Reproduction, Fertility and Development, 16: 133.
  
  
  • Sun, M. and Zigman, S. (1978). Determination of superoxide dismutase in erythrocytes using the method of adrenaline auto oxidation.  Analytical Biochemistry, 90: 81-89.
  
  
  • Uysal, O., Bucak, M.N., Yavas, I. and Varıslı, O. (2007). Effect of various antioxidants on the quality of frozen–thawed bull semen. Journal of Animal and Veterinary Advances, 6(2): 1362-1366.
  
  
  • Zini, A.M., Fischer, A., Mak, V., Phang, D. and Jarvi, K. (2002). Catalase-like and superoxide dismutase-like activities in human seminal plasma. Urological Research, 30: 321-323.