Effects of cysteamine and vitamin E addition to semen extender on post-thawed microscopic sperm parameters of buffalo bulls

Subject Areas : Veterinary Clinical Pathology

rahim  beheshti ¹ (دانشگاه آزاد اسلامی، واحد شبستر، استادیار گروه علوم درمانگاهی، شبستر، ایران.)
jamshid  ghiyasi ² (دانشگاه آزاد اسلامی، واحد شبستر، استادیار گروه علوم پایه، شبستر، ایران.)

Received: 2013-11-03 Accepted : 2014-06-24 Published : 2014-02-20

Keywords: vitamin E, Buffalo, Semen freezing, Cysteamine,

Abstract :

   This study was designed to investigate the effect of cysteamine and vitamin E on post thaw buffalo bull's sperm quality. For this purpose, 20 ejaculates from four buffalo bulls possessing more than 70% visual sperm motility were diluted at 37◦C in BioXcell® extender. The diluted semen was cooled to 4◦C within 2 hours, equilibrated at 4◦C following the addition of (0.75, 1.5, 2 and 5 mM) of vitamin E and (7.5, 12.5, 15 and 20 mM) cysteamine per 90 ml, filled in 0.5 ml French straws and were subjected to cooling condition before being plunged into liquid nitrogen. Semen was thawed at 37◦C for 40 seconds after 72 hours of storage inside liquid nitrogen .Post-thaw sperm motility and some qualitative parameters of each frozen semen sample were assessed by using computer assisted semen analyzer (CASA). In general, the results showed that the addition of 1.5 mM vitamin E and 20 mM cysteamine in the commercial diluents BioXcell extender for freezing buffalo semen increased the motility of spermatozoa and some qualitative parameters to post-thawed buffalo sperm.

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