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Open Access Article
1 - Study effect of methanolic extract of Peganum Harmala and Harmine on Kidney tissue damage of diabetes mellitus in male Wistar rats
Forough Kajbaf Shahrbanoo Oryan Ramesh Ahmadi Akram EidiIntroduction: The dangerous side effects of diabetes are diabetic nephropathy, which has a relatively high prevalence in diabetic patients and causes renal failure. Investigating the process of destruction and the mechanisms that prevent it, in order to reduce the tissu MoreIntroduction: The dangerous side effects of diabetes are diabetic nephropathy, which has a relatively high prevalence in diabetic patients and causes renal failure. Investigating the process of destruction and the mechanisms that prevent it, in order to reduce the tissue damage, especially with the treatment of plants, can be a significant issue. Material and methods: In this research, Espand with scientific name of Peganum harmala, a plant of the Zygophyllaceae family and its effective substance called harmine in diabetes-induced renal injury, were studied on 80 male Wistar rats in experimental and diabetic groups. Rats were treated with methanolic extract of seeds and leaves of Peganum Harmala and Harmine. After tissue passage and fixation, Microscopic slides were prepared and studied. In tissue studies, four factors of epithelial changes, tubular destruction, leukocyte inflammation and capillary obstruction were detected in the kidneys. Results: The diabetic rat group received harmine and the diabetic rats receiving the extract of the seed were less damaged, while in the diabetic rats recieveing the leaf extract these lesions was higher and it was interesting to note that there was no difference in the experimental groups with the positive control group. Conclusion: According to the tissue results in our study, seed extract and harmine have the ability to prevent tissue damage caused by diabetes, which can be attributed to the harmine alkaloid in the seed. Manuscript profile -
Open Access Article
2 - In vitro assessment of antimicrobial effect of methanolic extract of Peganum harmala against some important foodborne bacterial pathogens
طیبه zeinali محمد Mohsenzadeh رویا Rezaeian-Doloei رویا NabipourFoodborne bacterial pathogens play an important role in food infections/intoxications in human population. With ever increasing the number of antibiotic-resistant bacterial strains, there is an attempt to use the antimicrobial properties of herbs. Peganum harmala is a m MoreFoodborne bacterial pathogens play an important role in food infections/intoxications in human population. With ever increasing the number of antibiotic-resistant bacterial strains, there is an attempt to use the antimicrobial properties of herbs. Peganum harmala is a medicinal plant of Iraniantraditional medicine which was used as an antiseptic in the past. Amongthe foodborne bacterial pathogens, Escherichia coli O157:H7, Salmonella Typhimurium and Listeria monocytogenes are considered as the most important and hazardous pathogens. The aim of this study was to evaluate the antibacterial effect of methanolic extract of Peganum harmala against these bacteria in vitro. The minimum inhibitory concentration (MIC) and minimum bactericidal concentrations (MBC) of methanolic extract of Peganum harmala was determined against three foodborne bacterial pathogens by micro-dilution method in Muller-Hinton broth. According to the results, MIC for E. coli O157:H7 and S. Typhimurium was 1.56 mg/ml. In the case of L. monocytogenes, it was estimated at 0.78 mg/ml. Moreover, results revealed that MBC for these organisms was similar to MIC concentrations. Regarding the results, Peganum harmala can be used as an ingredient in the formula of the disinfectants applied in the food systems. Manuscript profile -
Open Access Article
3 - The study and comparison of phytochemical and antioxidant activity of various extracts of Dracocephalum moldavica L.in Sistan and Baluchestan province
monireh mehrabi norolla hazeri Jafar Valizadeh Moharam ValizadehDracocephalum moldavica L. is an annual herb belongs to Lamiaceae family, which has been used to treat of gastrointestinal, liver disorders and headaches.In this study, flowering branches of plant were collected from the greenhouse of the university of Sistan and Baluch MoreDracocephalum moldavica L. is an annual herb belongs to Lamiaceae family, which has been used to treat of gastrointestinal, liver disorders and headaches.In this study, flowering branches of plant were collected from the greenhouse of the university of Sistan and Baluchestan in March 2016. The methanol, ethanol, and aqueous extracts of plant were obtained by using maceration method. The amount of total phenolic, flavonoid and anthocyanin contents were evaluated by spectrophotometric methods and the antioxidant activity of the extracts were determined by FRAP and DPPH methods. The results were showed that the phenolic and flavonoid contents different of methanol, ethanol and aqueous extracts were (82.10, 71.8and33.65 mg GAE/g) and (34.02, 28.8and1.02mg QUE/g), respectively. In the evaluation of antioxidant activity by DPPH, the inhibitory power of methanol, ethanol and aqueous extracts were (11.60, 34.93 and 298.96 μg/ml) respectively, and (14.23, 8.20 and 1.01 mM Fe2+/ mg) respectively, sample in FRAP assay. The methanol extract of plant had the greatest content of phenolic (82.10 mg GAE / g), flavonoids (34.02 mg QUE/g) and anthocyanin(113.55μg/g) and antioxidant activity compared to ethanol and aqueous extracts, especially it also had the greatest DPPH inhibitory power (IC50= 11.60 μg/ml). Manuscript profile -
Open Access Article
4 - بررسی فیتوشیمیایی و مقایسه عملکرد آنتیاکسیدانی اسانس و عصاره گیاه Salvia reuterana Boiss. جمعآوری شده از منطقه دماوند (شمال ایران)
منصوره امین زاده امیر حسین جمشیدی فایزه السادات مرتضوی مقدم حسین ادزنیوند محمد رضا نقوی رحیم سروستانی -
Open Access Article
5 - بررسی محتوای فنولی اسانس و فلاونوئیدی عصارۀ شش گونه از جنس Artemisia در استانهای خراسان، سمنان و آذربایجان غربی
محبوبه طاهرخانی -
Open Access Article
6 - Effects of Pimpinella saxifraga L. extracts on environmental isolates of Enterococcus faecalis and HeLa cell line
Fatemeh Shams Moattar Mirsassan Mirpour Naghmeh HemmatiPimpinella saxifraga L. is a genus of aromatic plants native to northern Iran. Some of its medicinal benefits are already known. The purpose of this study is to investigate the effect of Pimpinella saxifraga L. plant extract on some environmental isolates of Enterococcu MorePimpinella saxifraga L. is a genus of aromatic plants native to northern Iran. Some of its medicinal benefits are already known. The purpose of this study is to investigate the effect of Pimpinella saxifraga L. plant extract on some environmental isolates of Enterococcus faecalis and the HeLa cell line. After collecting the plant and drying it, methanolic and aqueous extracts were prepared. Since phenolic compounds have multiple effects, the number of phenolic compounds in the methanolic extract was measured by the Folin-ciocalteu method. Antibacterial activity against E. faecalis was first measured by disk diffusion, and then MIC and MBC were measured by the Microdilution method. Finally, the anti-proliferative activity of plant extracts on the cervical cancer cell line (HeLa) was investigated using the MTT method. The volume of phenolic compounds in the methanolic extract was 968.33 mg/ml. The investigation of the antimicrobial effect showed that the methanolic extract has significant effects against E. faecalis. The MIC and MBC of this extract were 25 and 100 mg/ml, respectively, and the growth of the cervical cancer cell line (HeLa) was inhibited by about 13% by using MIC concentration. The results of the experiments demonstrated that the methanolic extract of P. saxifraga L. has an inhibitory effect on the growth and proliferation of Enterococcus faecalis and the HeLa cell line. Manuscript profile -
Open Access Article
7 - ارزیابی اثر آنتی اکسیدانی عصاره کنجاله روغن کشی شده کنجد جهت پایدار سازی روغن های خوراکی
رضا صفاری امیر حسین الهامی راد اسماعیل عطای صالحی -
Open Access Article
8 - Antibacterial effect of methanolic extract of Eryngium Caeruleum and Mentha Spicata on Staphylococcus aureus in a food model at 15˚C
Fatemeh Amini Hamdollah Moshtaghi Maryam AbbasvaliIn this interventional investigation, the effect of methanol extract of Eryngium Caeruleum and Mentha Spicata (5% and 10% v/w) on Staphylococcus aureus in soy cheese at 106 cfu/ml at 15˚C for a period of 15 days was studied. Methanol extract of Eryngium caeruleum and Me MoreIn this interventional investigation, the effect of methanol extract of Eryngium Caeruleum and Mentha Spicata (5% and 10% v/w) on Staphylococcus aureus in soy cheese at 106 cfu/ml at 15˚C for a period of 15 days was studied. Methanol extract of Eryngium caeruleum and Mentha Spicata was prepared by soaking dried plants in 85% methanol. Soy cheese was made through coagulating of soy milk by calcium sulfate. Results were analyzed by one-way variance, ANOVA. In 15˚C, Staphylococcus aureus was faced with reduction in both concentrations (5% and 10% v/w) of methanol extract of E. caeruleum i.e. 1 and 2 log, and in both concentrations (5% and 10% v/w) of methanol extract of M. Spicata i.e. 2 and 4 log, respectively. The antibacterial effect of 10% extract of both of the plants on Staphylococcus aureus was more than 5% extract. Generally, results suggested that Eryngium caeruleum and Mentha Spicata have antibacterial effect, on Staphylococcus aureus. Manuscript profile -
Open Access Article
9 - Study the antimicrobial effects of methanolic extract of Hypericum perforatum on foodborne bacteria
Leila Ghodrati Mehrdad Ataie Kachoie Sadegh Mousavi-Fard Fariborz MoattarHypericum perforatum is a medicinal plant of the genus Hypercasia that has phenolic and flavonoid compounds with high antimicrobial and antioxidant properties. The present study was performed to evaluate the antimicrobial effects of the methanolic extract of Hypericum p MoreHypericum perforatum is a medicinal plant of the genus Hypercasia that has phenolic and flavonoid compounds with high antimicrobial and antioxidant properties. The present study was performed to evaluate the antimicrobial effects of the methanolic extract of Hypericum perforatum on food-borne bacteria. Aerial part of Hypericum perforatum was prepared from the research farm, and after approval by experts, it was dried and powdered and used to prepare methanolic extract. The diameter of the growth inhibition zone of Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus was assessed using disk diffusion and compared with antibiotics. Minimum inhibitory concentration (MIC) and Minimum Bacterial Concentration (MBC) of methanolic extract of Hypericum perforatum were evaluated on the target bacteria using ELISA plate. The diameter of the growth inhibition zone of bacteria against the methanolic extract of Hypericum perforatum ranged from 9.33±0.45 to 15.28±0.60 mm. Application of 100 mg/ml concentration of methanolic extract of Hypericum perforatum caused the highest diameter of the growth inhibition zone of Pseudomonas aeruginosa (12.27±0.53 mm), Escherichia coli (13.20±0.59 mm) and Staphylococcus aureus (15.28±0.60 mm). The antimicrobial effects of methanolic extract of fenugreek were dose-dependent (P <0.05). The lowest and highest levels of MIC and MBC of methanolic extract of Hypericum perforatum were obtained for Staphylococcus aureus (0.0010 and 0.0019, respectively) and Escherichia coli (0.50 and 1.00, respectively). The diameter of the growth inhibition zone of bacteria treated with methanolic extract of Hypericum perforatum was higher compared to some antibiotic discs. Manuscript profile -
Open Access Article
10 - Evaluation of antibacterial effects of Onions, methanol extracts and some antibiotics against the number of food born bacteria
yones Anzabi Afshin javadiIn this study, antibacterial effects of methanol extracts of red Onions with the scientific name; Allium cepa L.by the method of determining of MIC and MBC andthen perform antibiotic susceptibility test by the agar disk diffusion method onthe standard strains of some ba MoreIn this study, antibacterial effects of methanol extracts of red Onions with the scientific name; Allium cepa L.by the method of determining of MIC and MBC andthen perform antibiotic susceptibility test by the agar disk diffusion method onthe standard strains of some bacteria in terms of food hygiene and were compared with the effects of some antibiotics.Results showed that the highest inhibitory effect was on Yersinia enterocolitica and Clostridium perfrigenes (MIC=62.5µg/mL) and the lowest same effect was on Staphylococcos aureus(MIC=500µg/mL).As well as the most bactericidal effect was on Yersinia enterocolitica (MBC=62.5µg/mL) and the lowest same effect was on Staphylococcos aureus (MBC=1000µg/mL).Also the results ofantibiogram tests indicated that the onions, methanol extracts have optimum antibacterial effect to the effects of the Vancomycin and Nalidixic acid as standard antibiotics and the greatest impact of this terms submitted on Clostridium perfrigenes(gram-positive bacteria) and Yersinia enterocolitica(gram-negative bacteria) thatstatistically; in the range of p Manuscript profile -
Open Access Article
11 - ارزیابی فعالیت ضد باکتریایی عصاره متانولی گیاه کک گریز در دو مرحله برداشت بر روی تعدادی از باکتریهای هوازی عامل عفونت های واژینال
لیلا فیروزیان لیلا امجد منیر دودیBackground & Aim:Vaginal disease has always been a great concern of women. Medicinal plants as natural resources with fewer side effects can effectively treat these infections given the diversity of these infections, resistance to antibiotics and side effects of exi MoreBackground & Aim:Vaginal disease has always been a great concern of women. Medicinal plants as natural resources with fewer side effects can effectively treat these infections given the diversity of these infections, resistance to antibiotics and side effects of existing drugs. The present study aimed to compare antimicrobial effects of methanol extract of Francoeuria Undulata on growth of several gram-positive and gram-negative bacteria causing vaginal infections. Experimental: Francoeuria Undulata as an indigenous Iranian plant was collected in two harvest times (T1 and T2). Methanol extract of aerial parts of the plant was prepared. The effects of various concentrations (62.5, 125, 250 and 500 mg/ml)of methanol extracts were studied onKlebsiella and Enterobacter as gram-negative pathogens. Minimum Inhibitory Concentration (MIC) of bacterial growth and Minimum Bactericidal Concentration (MBC) were determined using macro-dilution method. Data analysis was performed using ANOVA. Results: The results showed that methanol extracts of aerial parts of Francoeuria Undulata inhibit bacterial growth of Klebsiella pneumoniae and Enterobacter aerogenes. MICs varied from 125mg/ml to 250 mg/ml in the old and new harvest times. Therefore, the smallest diameter of inhibition zone belonged to Klebsiella pneumoniae at 62.5mg/ml concentration in the old harvest time and the largestdiameter of inhibitory zone belonged tothe second strains of Klebsiellapneumoniae at 500mg/ml concentration in the new harvest time. Recommended applications/industries: The findings of the study indicated that methanol extracts of Francoeuria Undulata at two old and new harvest times had strong antibacterial and inhibitory effects on gram-negative bacteria causing vaginal infections. Manuscript profile -
Open Access Article
12 - ارزیابی فعالیت های ضد میکروبی و سمیت سلولی عصاره های متانولی برگ گیاه Sansevieria zeylanica (L.) Willd. روی سرطان سینه در انسان، لاین های سلولی MDA-MB-231
S. Rajashekara S. Chudamani S. Rautela Sharvani Hegde S. Swaroopaگیاه Sansevieria zeylanica یک گونه علفی چند ساله مناطق خشک است که در قسمت­های گرمسیر و نیمه گرمسیر دنیا پراکندگی دارد. این گیاه، یک گیاه شگفت­انگیز دارویی است و مزایای زیادی دارد. هدف این مطالعه تعیین فعالیت­های سمیت سلولی در سرطان سینه در انسان روی لاین­ Moreگیاه Sansevieria zeylanica یک گونه علفی چند ساله مناطق خشک است که در قسمت­های گرمسیر و نیمه گرمسیر دنیا پراکندگی دارد. این گیاه، یک گیاه شگفت­انگیز دارویی است و مزایای زیادی دارد. هدف این مطالعه تعیین فعالیت­های سمیت سلولی در سرطان سینه در انسان روی لاین­های سلولی MDA-MB-231 و نیز ارزیابی خاصیت ضد میکروبی با استفاده از عصاره برگ متانولی گیاه S. zeylanica است. آزمایش اصلی تعیین حساسیت میکروب­ها در برابر عصاره­های متانولی برگ گیاه S. zeylanica است. درصد بازدارندگی رشد اندازه­گیری شد و غلظت نمونه برای ممانعت از رشد سلول تا 50 درصد (IC50) برای درصد زنده­مانی تعیین شد. عصاره­های برگی بدست آمده از گیاه S. zeylanica روی لاین­های سلولیMDA-MB-231 سرطان سینه در انسان اثر سمیت سلولی نشان دادند. شاخص IC50 (78/1167 میکروگرم در میلی­لیتر) پس از تیمار 24 ساعته روی این لاین­ها بدست آمد. افزایش مرگ سلولی روی لاین­های MDA-MB-231 با افزایش غلظت عصاره نشان می­دهد که عصاره متانولی برگ این گیاه فعال است. برای اولین بار، ما تلاش کردیم تا خصوصیات بیولوژیکی عصاره برگ متانولی گیاه S. zeylanica را ارزیابی کنیم. نتایج حاصل کاربرد درمانی را تایید کرد و می­تواند برای کاربرد وسیع به­عنوان ماده­ای ضد میکروب، مدرک معتبری باشد. در این مطالعات نتایج غربال­گری فعالیت­های فایتوشیمیایی، ضد میکروبی و سمیت گیاه S. zeylanica تایید کرد که این گیاه می­تواند به­عنوان منبع گیاهی طبیعی مورد استفاده قرار گیرد. این گیاه می­تواند برای مصارف دارویی و داروشناسی در مقیاس وسیع و بصورت صنعتی استفاده شود. Manuscript profile -
Open Access Article
13 - The Effect of Oral Cyclosporine on Plasma Levels of Parathormone and Calcitonin in Adult Male Rat
غلامحسن واعظی ریحانه پوزش محمد رضا خواجه دلوییFear is known as an emotional response to the threat and included autonomic, neuroendocrine and behavioral responses. Many scientists have discovered the healing properties of plants and recourse to medicinal herbs always through history has was been one of the ways tre MoreFear is known as an emotional response to the threat and included autonomic, neuroendocrine and behavioral responses. Many scientists have discovered the healing properties of plants and recourse to medicinal herbs always through history has was been one of the ways treatment effective. In this research, was studied the comparison of injection cardamomum seed aqueous and methanolic extracts on fear behavior induced by PTZ. In this study, animals were divided in to two defferent groups, each group was comprised of three groups.Control group that 1lit, saline received (i.c.v). Sham group that received 20 mg/kg PTZ (i.p). The experimental group, were received doses 3,10,30 g/rat of cardamomum seed aqueous extract (i.c.v) and PTZ(i.p), too experimental two group, were received doses 3,10,30 g/rat of methanolic extract (i.c.v) and 20 mg/kg PTZ(i.p). The fear effect was evaluated by using an Elevated plus-maze. Injection comparison of cardamomum seed aqueous and methanolic extracts, increased open arm time percentage (%OAT) ,open arm entries percentage (%OAE) and locomotor activity that 30 g/rat dose increased in % OAT, % OAE and % CATsignificantly. The results show that compounds in cardamomum seed in aqueous and methanolic extracts decrease fear induced by PTZ probably through interaction with gabaergic system. Manuscript profile -
Open Access Article
14 - Comparative Effect of Timolol and Methanolic Extract of Zataria multiflora with Vitamin E on APO E Gene Expression and Behavioral and Biochemical Changes in Alzheimer's Rats
Samira Homayoun Pour Maryam Bananej Maryam Khosravi Hengameh AlibeikAlzheimer's Disease is the most common form of dementia, characterized by memory and cognitive deficits. Vitamin E, as an antioxidant, plays an important role in reviving free radicals and converting them into safe substances by giving hydrogen. Shirazi thyme (Zataria m MoreAlzheimer's Disease is the most common form of dementia, characterized by memory and cognitive deficits. Vitamin E, as an antioxidant, plays an important role in reviving free radicals and converting them into safe substances by giving hydrogen. Shirazi thyme (Zataria multiflora) has antibacterial, antifungal and antioxidant properties. The aim of this study is the comparative effect of timolol with methanol extract of Zataria multiflora with vitamin E on Alzheimer's disease in male Wistar rats. 40 male Wistar rats weighing approximately 200 grams were randomly divided into 5 groups of eight. Beta-amyloid 42 was used to induce Alzheimer's disease. Then, methanolic and timolol extracts were extracted from Zataria multiflora. The level of Apo E gene expression was evaluated by Real Time PCR and TC, LDL and HD biochemical factors. The expression of Apo E gene in the experimental groups increased significantly compared to the Alzheimer's group. The expression of this gene was decreased in the sham group compared to the control group. The group of Alzheimer's mice had a significant increase in total cholesterol (TC) and low-density lipoprotein (LDL) serum levels, and the level of HDL showed a significant decrease compared to the control group. Timolol, m ethanolic extract of Zataria multiflora and vitamin E have positive effects on the expression of APOA E gene in leukocytes. On the other hand, with a significant effect on HDL, the methanolic extract of Zataria multiflora can be used as an effective product to prevent and reduce the complications of Alzheimer's disease. Manuscript profile
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