Effects of Pimpinella saxifraga L. extracts on environmental isolates of Enterococcus faecalis and HeLa cell line

Subject Areas : Biotechnological Journal of Environmental Microbiology

Fatemeh Shams Moattar ¹ , Mirsassan Mirpour ² , Naghmeh Hemmati ³

1 - Department of Microbiology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University, Lahijan, Iran
2 - Department of Microbiology,Faculty of Basic Sciences,Lahijan Branch, Islamic Azad University, Lahijan, Iran.
3 - Department of Microbiology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University, Lahijan, Iran.

Received: 2022-10-04 Accepted : 2023-02-01 Published : 2022-07-01

Keywords: Antibacterial effect, Hela cell line, Enterococcus faecalis, Methanolic extracts, Pimpinella saxifraga L,

Abstract :

Pimpinella saxifraga L. is a genus of aromatic plants native to northern Iran. Some of its medicinal benefits are already known. The purpose of this study is to investigate the effect of Pimpinella saxifraga L. plant extract on some environmental isolates of Enterococcus faecalis and the HeLa cell line. After collecting the plant and drying it, methanolic and aqueous extracts were prepared. Since phenolic compounds have multiple effects, the number of phenolic compounds in the methanolic extract was measured by the Folin-ciocalteu method. Antibacterial activity against E. faecalis was first measured by disk diffusion, and then MIC and MBC were measured by the Microdilution method. Finally, the anti-proliferative activity of plant extracts on the cervical cancer cell line (HeLa) was investigated using the MTT method. The volume of phenolic compounds in the methanolic extract was 968.33 mg/ml. The investigation of the antimicrobial effect showed that the methanolic extract has significant effects against E. faecalis. The MIC and MBC of this extract were 25 and 100 mg/ml, respectively, and the growth of the cervical cancer cell line (HeLa) was inhibited by about 13% by using MIC concentration. The results of the experiments demonstrated that the methanolic extract of P. saxifraga L. has an inhibitory effect on the growth and proliferation of Enterococcus faecalis and the HeLa cell line.