Effects of Pimpinella saxifraga L. Extracts on Environmental Isolates of Enterococcus faecalis and HeLa Cell Line
محورهای موضوعی : Biotechnological Journal of Environmental Microorganisms
فاطمه شمس معطر
1
,
میرساسان میرپور
2
,
نغمه همتی
3
1 - گروه میکروبیولوژی ، دانشکده علوم پایه ، واحد لاهیجان ،دانشگاه آزاد اسلامی ، لاهیجان ، ایران
2 - گروه میکروبیولوژی دانشکده علوم پایه ، واحد لاهیجان ، دانشگاه آزاد اسلامی ، لاهیجان ، ایران
3 - گروه میکروببولوژی ، دانشکده علوم پایه ، واحد لاهیجان ،دانشگاه آزاد اسلامی ، لاهیجان ، ایران
کلید واژه: Antibacterial effect, Enterococcus faecalis, HeLa cell line, Methanolic extracts, Pimpinella saxifraga L.,
چکیده مقاله :
Pimpinella saxifraga L. is a genus of aromatic plants native to northern Iran. Some of its medicinal benefits are
already known. The purpose of this study is to investigate the effect of Pimpinella saxifraga L. plant extract
on some environmental isolates of Enterococcus faecalis and the HeLa cell line. After collecting the plant and
drying it, methanolic and aqueous extracts were prepared. Since phenolic compounds have multiple effects,
the number of phenolic compounds in the methanolic extract was measured by the Folin-Ciocalteu method.
Antibacterial activity against E. faecalis was first measured by disk diffusion, and then MIC and MBC were
measured by the microdilution method. Finally, the anti-proliferative activity of plant extracts on the cervical
cancer cell line (HeLa) was investigated using the MTT method. The volume of phenolic compounds in the
methanolic extract was 968.33 mg/ml. The investigation of the antimicrobial effect showed that the methanolic
extract has significant effects against E. faecalis. The MIC and MBC of this extract were 25 and 100 mg/ml,
respectively, and the growth of the cervical cancer cell line (HeLa) was inhibited by about 13% by using the
MIC concentration. The results of the experiments demonstrated that the methanolic extract of P. saxifraga L.
has an inhibitory effect on the growth and proliferation of Enterococcus faecalis and the HeLa cell line.
Pimpinella saxifraga L. is a genus of aromatic plants native to northern Iran. Some of its medicinal benefits are
already known. The purpose of this study is to investigate the effect of Pimpinella saxifraga L. plant extract
on some environmental isolates of Enterococcus faecalis and the HeLa cell line. After collecting the plant and
drying it, methanolic and aqueous extracts were prepared. Since phenolic compounds have multiple effects,
the number of phenolic compounds in the methanolic extract was measured by the Folin-Ciocalteu method.
Antibacterial activity against E. faecalis was first measured by disk diffusion, and then MIC and MBC were
measured by the microdilution method. Finally, the anti-proliferative activity of plant extracts on the cervical
cancer cell line (HeLa) was investigated using the MTT method. The volume of phenolic compounds in the
methanolic extract was 968.33 mg/ml. The investigation of the antimicrobial effect showed that the methanolic
extract has significant effects against E. faecalis. The MIC and MBC of this extract were 25 and 100 mg/ml,
respectively, and the growth of the cervical cancer cell line (HeLa) was inhibited by about 13% by using the
MIC concentration. The results of the experiments demonstrated that the methanolic extract of P. saxifraga L.
has an inhibitory effect on the growth and proliferation of Enterococcus faecalis and the HeLa cell line.
