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Open Access Article
1 - Prevalence study of Pseudomonas aeruginosa septicemia in broiler in Mazandaran
سیدمحمد حسینی تقی زهرائی صالحی مجتبی شالیزار مرتضی شعبانی عارف ابراهیم زاده شیلا امیدظهیرPseudomonas aeruginosa is the most common avian pathogens can cause localized or systemicdiseases in young and growing poultry. Morbidity and mortality varies from 2 to 100%, butmore commonly about 2-12% with greatest losses in very young birds. The disease occurred in6 MorePseudomonas aeruginosa is the most common avian pathogens can cause localized or systemicdiseases in young and growing poultry. Morbidity and mortality varies from 2 to 100%, butmore commonly about 2-12% with greatest losses in very young birds. The disease occurred in6-10 days-old ross broiler chickens. Clinical signs showed sleepy appearance, closed eyes, mildkeratitis and catarrhal rhinitis. Gross lesions revealed airsacculitis, congestion and swollen ofkidneys with deposition of ureats in the urters and proctitis. The disease morbidity and mortalityrates were 16.2% and 6%, respectively. Microbial culture was determined motile and Gramnegativebacteria, suspected greenish colonies, smell like fruity and beta hemolysis on bloodagar. Consequently, we suspected to Pseudomoniasis after culture and clinical signs finding.These isolates were transmitted to the department of microbiology, faculty of veterinarymedicine, university of Tehran and all isolates were identified by microbiological phenotypiccharacteristics with conventional methods and Pseudomonas aeruginosa was finally diagnosed. Manuscript profile -
Open Access Article
2 - Evaluation of metallo-betalactamase and carbapenemase activity in Pseudomonas aeruginosa strain isolated from clinical samples in Tehran
سمیه Mashayekhy فاطمه Noorbakhsh سحر Honarmand JahromiPseudomonas aeruginosa is an opportunistic pathogen and a major cause of nosocomial infections. Metallobetalactamases and carbapenemases are the most important factors in resistance to carbapenem drugs in Pseudomonas aeruginosa strains. The aim of this study was to eval MorePseudomonas aeruginosa is an opportunistic pathogen and a major cause of nosocomial infections. Metallobetalactamases and carbapenemases are the most important factors in resistance to carbapenem drugs in Pseudomonas aeruginosa strains. The aim of this study was to evaluate the activity of metallobetalactamase and carbapenemase in Pseudomonas aeruginosa isolated from clinical specimens. 49 strains of Pseudomonas aeruginosa isolated from patients admitted to the intensive care unit were identified by biochemical methods, then their antibiotic susceptibility was determined by Kirby- Bauer method. MBL producing strains were identified by phenotypic method combined disk test and KPC- producing strains were evaluated by MHT method. PCR method was also used to identify strains carrying VIM, SIM, GIM, SPM and IMP genes. Antibiotic resistance to ticarcillin, meropenem, gentamicin, ciprofloxacin, cefpime and ceftazidime were 89.8%, 51%, 44.9%, 67.4%, 93.9%, 95.9%, respectively. By phenotypic analysis combined disk test, 55.1% of the strains were identified as metallo-betalactamase producing strains. Also, 38.8% of carbapenemase producing strains were observed by MHT method. The frequencies of each of these gene’s VIM, SIM and GIM were 63.3%, 38.8%, 34.7%, respectively, and SPM and IMP genes were not observed in any of the strains in this study. Manuscript profile -
Open Access Article
3 - مقایسه خاصیت آنتی باکتریایی رنگدانه پرودیجیوزین باکتری سراشیا مارسسنس و رنگدانه کاروتنوئیدی رودوترولا بر روی سودوموناس آئروژینوزا و آسنیتوباکتر بومانی
بهاره رحیمیان ظریف زهرا کامیابی منصور بیات -
Open Access Article
4 - اثر ضد باکتریایی نانوذرات نقره سنتز شده از عصاره گیاه گزنه Urtica diorica بر استافیلوکوکوس اورئوس و سودوموناس آئروژینوزا
امید زمانی زهرا کشتمند سیدمحمد مهدی حمدی -
Open Access Article
5 - بررسی تنوع ژنتیکی جدایه های سودوموناس آئروژینوزا بدست آمده از موارد ورم پستان گاو در شهر تبریز
سامان مهدوی -
Open Access Article
6 - بررسی اثر ملیتین استخراج شده از زهر زنبور عسل ایرانی روی سویه‌های کلینیکی سودوموناس آئروژینوزا
مرضیه رضایی منصور بیات دلاور شهباز زاده محسن مومن زاده رضا اکبری کامران پوشنگ باقری -
Open Access Article
7 - Determine the antimicrobial susceptibility of Pseudomonas aeruginosa strains isolated from raw milk
faeze zadsafar MOHSEN ZARGAR سید سهیل آقاییAbstractBackground and Objectives: Pseudomonas aeruginosa, a pathogen bacteria, that is resistance to antibiotics and can be used in patients with immune deficiency and cause disease. This study aimed to determine the antimicrobial susceptibility of Pseudomonas aerugino MoreAbstractBackground and Objectives: Pseudomonas aeruginosa, a pathogen bacteria, that is resistance to antibiotics and can be used in patients with immune deficiency and cause disease. This study aimed to determine the antimicrobial susceptibility of Pseudomonas aeruginosa strains isolated from raw milk and multiple resistance of the isolates and ESBL isolates.Method: Initially, 117 Pseudomonas aeruginosa isolates from dairy products were confirmed by the standard methods of microbiology and then evaluated the antibiotic resistance of 10 strains against 10 antibiotic which selected from different categories according to the CLSI standard. Results: In the study of antibiotic resistance Ceftazidime antibiotic showed most Antibiotic resistance and amikacin antibiotic polymyxin B antibiotic resistance and lowest, respectively.27 isolates (23%), multiple drug resistance (MDR), the three classes of antibiotics showedand 86 isolates were resistant to the ceftazidime antibiotic (73.5%) isolates, of which 28 were positive ESBL (32.55%). Conclusion: Due to high prevalence Pseudomonas aeruginosa in raw milk, and existence of antibiotic resistance genes in bacteria, it is necessary to apply appropriate strategies for livestock health control, to prevent the spread of bacteria. Manuscript profile -
Open Access Article
8 - Molecular detection of DHA beta-lactamase gene in Pseudomonas aeruginosa isolated from skin samples in Qom
Horisadat Hejazi Mohsen Zargar mehrdad mamarianIntroduction Pseudomonas aeruginosa can infect virtually all tissues in the body. this bacteria can be colonized in burns, wounds, corneal tissue, urinary tract and lungs, and after entering blood, cause septicemia or localized lesions in other parts of the body. Today, MoreIntroduction Pseudomonas aeruginosa can infect virtually all tissues in the body. this bacteria can be colonized in burns, wounds, corneal tissue, urinary tract and lungs, and after entering blood, cause septicemia or localized lesions in other parts of the body. Today, due to the untapped use of effective drugs On the pseudomonas aeruginosa, this bacterium is strongly resistant to antibiotics. The purpose of this study was to find the gene for resistance to beta-lactams in pseudomonas aeruginosa isolated from skin samples. materials and methods: A total of 100 isolates of Pseudomonas were used in Microbiology Bank of Microbiology Research Laboratory of Islamic Azad University of Qom. After cultivation and biochemical confirmation of isolates, genomic extraction was performed first to detect these genes and then detected by PCR with DHA genes. Results: 45 isolates resistant to imipenem; 22 cases were positive in the double-synergistic disks. Six isolates were positive for DHA gene. Conclusion: Increasing resistance to Pseudomonas aeruginosa causes problems in treating patients and a periodic review of drug allergy for inappropriate treatment. Manuscript profile -
Open Access Article
9 - Using a phenotypic method to confirm the presence of the efflux pump associated with resistance to ciprofloxacin in Pseudomonas aeruginosa isolated from skin samples
Ali Rahmati Mohammad Reza zolfagharyIntroduction One of the most important causes of nosocomial infections in patients with skin infections is Pseudomonas aeruginosa. The occurrence of antibiotic resistance with various mechanisms is one of the problems in treating these patients. The main role in the de MoreIntroduction One of the most important causes of nosocomial infections in patients with skin infections is Pseudomonas aeruginosa. The occurrence of antibiotic resistance with various mechanisms is one of the problems in treating these patients. The main role in the development of multiple resistance to antimicrobial drugs is played by the MexAB efflux pumps. The aim of this study was to evaluate the presence of the MexAB efflux pumps in the Pseudomonas aeruginosa isolated from skin samples. Materials and Methods: In this study 200 swab specimens from patients with skin infections were collected in Qom hospitals. Pseudomonas aeruginosa specimens were confirmed by biochemical tests. The drug susceptibility pattern was compared to ciprofloxacin by disk diffusion method and the phenotypic study of the effects of efflux pumps was done using cartwheel method . Results: In this study, 89 (45%) samples were infected with Pseudomonas aeruginosa, of which 19 cases (21%) were resistant to ciprofloxacin and more than 54% of the presence of the pump in the Pseudomonas aeruginosa more than 54% of the presence of the pump in the Pseudomonas aeruginosa. Conclusion: the presence of more than 54% of the presence of the pump in the Pseudomonas aeruginosa, is important in order to suggest a suitable therapeutic model for the infected patients. Keywords: Pseudomonas aeruginosa, skin infections, efflux pump Manuscript profile -
Open Access Article
10 - The effect of silver nanoparticles on the expression of exotoxin A and S genes in Pseudomonas aeruginosa
Muhammad Hemati Zahra Keshtmand Katayoun BorhaniBackground: Pseudomonas aeruginosa is one of the most important causes of nosocomial infections and exotoxin genes are important in the development of this infection.On the other hand, with the advancement of nanotechnology, today, the use of nanoparticles has been wide MoreBackground: Pseudomonas aeruginosa is one of the most important causes of nosocomial infections and exotoxin genes are important in the development of this infection.On the other hand, with the advancement of nanotechnology, today, the use of nanoparticles has been widely considered in the field of medicine and medicine, especially against microbes. The aim of the present study was to investigate the effect of silver nanoparticles (AgNPs) on the expression of Pseudomonas aeruginosa exotoxin A and S genes. Methods: In this experimental study, the minimum growth inhibition concentration of silver nanoparticles was determined by microdilution method. First, the lowest concentration of bacterial growth inhibitor is determined with silver nanoparticles. Then, at a concentration lower than the lowest growth rate, the expression of Pseudomonas aeruginosa exotoxin A and S genes was examined by Real time PCR. Data were analyzed using one-way ANOVA, Tukey test and P value less than 0.05. Findings: Silver nanoparticles with a concentration of 62.5 μg / ml had growth inhibitory effects on Pseudomonas aeruginosa. Also, at a concentration of 31.25, the expression of Pseudomonas aeruginosa exotoxin A and S genes was significantly reduced (p < 0.05). Conclusion: The results of this study show that lower concentrations (31.25 μg/ml) than growth inhibition concentrations (62.5μg/ml) can reduce the expression of Pseudomonas aeruginosa exotoxin A and S genes. Manuscript profile -
Open Access Article
11 - A review of biofilm and chorus sensing formation in Pseudomonas aeruginosa
Rezvaneh behzadmehr Maryam Beigomi saeide saeidiPseudomonas aeruginosa is an opportunistic pathogen and causes 10 to 15% of nosocomial infections. The presence of unity genes is one of the most important invasive mechanisms of Pseudomonas aeruginosa and this issue is of special medical importance.The expression of ma MorePseudomonas aeruginosa is an opportunistic pathogen and causes 10 to 15% of nosocomial infections. The presence of unity genes is one of the most important invasive mechanisms of Pseudomonas aeruginosa and this issue is of special medical importance.The expression of many Pseudomonas aeruginosa pathogenic genes is controlled by a gene system called the Quorum Sensing System (QS). Chromosensing is a cell-to-cell communication system using small SMs molecules in single-celled organisms. The present review study was obtained with the help of articles indexed in Magiran and SID Persian scientific databases and Scopus, Google scholar, Ebscohost and Science Direct English databases.The QS system genes are highly abundant among human-derived Pseudomonas aeruginosa strains. It is also known as an infectious bacterium with high antibiotic resistance.The results showed that medicinal plants in different dilutions inhibit chromosome sensing and biofilm in bacteria.The results showed that medicinal plants in different dilutions inhibit chromosome sensing and biofilm in bacteria. Manuscript profile -
Open Access Article
12 - Effect of silver nanoparticles on surface hydrophobicity and biofilm formation in Staphylococcus aureus, Acinetobacter baumannii, Pseudomonas aeruginosa and Escherichia coli
alireza Farazandeh Fatemeh Noorbakhsh Sahar Honarmand JahromiIntroduction: Biofilms are microbial aggregates that adhere to a substrate as a surface and are surrounded by an exopolysaccharide matrix. The bacteria that make up biofilms are so resistant to antimicrobials and antibiotics that this has led to concerns in the medical MoreIntroduction: Biofilms are microbial aggregates that adhere to a substrate as a surface and are surrounded by an exopolysaccharide matrix. The bacteria that make up biofilms are so resistant to antimicrobials and antibiotics that this has led to concerns in the medical community.The aim of this study was effect of silver nanoparticles on surface hydrophobicity and biofilm formation in Staphylococcus aureus, Acinetobacter baumannii, Pseudomonas aeruginosa and Escherichia coli. Methods: This study was performed on 40 bacterial strains including Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli and Acinetobacter baumannii isolated from Milad Hospital in Tehran. The strains were identified by biochemical tests. Also, biofilm formation by microtiter plate method, surface hydrophobicity by MATH method and sensitivity of strains to silver nanoparticles were evaluated.Results: According to the results obtained in this study, all strains were able to form biofilms and no strains with negative biofilms were observed in the study. The highest effect of silver nanoparticles in this study was observed on strains at concentrations of 0.5, 1 and 2 μg / ml. Also, only 20% of Pseudomonas aeruginosa strains in this study were observed with moderate hydrophobicity and the others of the bacteria were weakly hydrophobic.Conclusion: silver nanoparticles in low concentrations with the effect of low toxicity had the ability to inhibit the biofilm formation of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Acinetobacter baumannii. Hydrophobicity in the studied bacteria were weak. Manuscript profile -
Open Access Article
13 - Evaluation of the effect of gold nanostructure on the production of bio surfactants from Pseudomonas aeruginosa
Yaser Ahsani Arani Zahra Noormohammadi behnam rasekh fatemeh Yazdian Hojjat kazemi Raminolipids belong to the group of glycolipid bio surfactants and were first isolated from Pseudomonas aeruginosa. Rhamnolipids are a good alternative to synthetic surfactants due to their low toxicity, biodegradability and selective performance. In this s More Raminolipids belong to the group of glycolipid bio surfactants and were first isolated from Pseudomonas aeruginosa. Rhamnolipids are a good alternative to synthetic surfactants due to their low toxicity, biodegradability and selective performance. In this study, the effect of gold (Au) nanoparticles on the growth and production of Pseudomonas aeruginosa PBCC5 bio surfactant was evaluated. Different concentrations of 1, 500 and 1000 mg / l nanoparticles were used. In this study, bio surfactant surface tension and emulsification indices (24E) were measured. The presence of nanoparticles on the bacterial surface was investigated by TEM and the morphology of nanoparticles was investigated by SEM. Binding of nanoparticles to bio surfactants was confirmed by TEM. The results showed that gold nanoparticles were not bactericidal and also increased bacterial growth and bio surfactant production. The surface tension of all samples was reduced from 72 mN / m distilled water to 35-32 mN / m. Manuscript profile -
Open Access Article
14 - In silico Analysis of Inhibitory Potential of Major non-Steroidal Anti-inflammatory Drugs against Las-quorum Sensing Circuit in Pseudomonas aeruginosa
Hossein Zahmatkesh Behnam RastiThe emergence of drug resistance, therapeutic failure, and the development of Pseudomonas aeruginosa infections are primarily attributed to biofilm formation and quorum sensing (QS) dependent virulence factors. The antimicrobial potential of some non-steroidal anti-infl MoreThe emergence of drug resistance, therapeutic failure, and the development of Pseudomonas aeruginosa infections are primarily attributed to biofilm formation and quorum sensing (QS) dependent virulence factors. The antimicrobial potential of some non-steroidal anti-inflammatory drugs (NSAIDs), such as ibuprofen, has been determined in laboratory studies. Herein, a docking analysis was conducted to examine the interaction between seven NSAIDs and the proteins of the Las system. Initially, the three-dimensional structure of selected NSAIDs (Diclofenac sodium, Ibuprofen, Ketoprofen, Mefenamic acid, Meloxicam, Naproxen, and Tenoxicam), and natural ligand of LasR (3-oxo-C12-HSL) were retrieved from PubChem database. Also, crystal structures of LasI Synthase and transcriptional activator protein LasR were obtained from Protein Data Bank. Subsequently, the molecular docking analysis utilizing AutoDock Vina software was employed to investigate the capability of the selected NSAIDs to inhibit the LasI/LasR receptor. Based on our findings, the majority of the selected NSAIDs exhibited favorable interactions with LasI/R proteins. Moreover, ketoprofen exhibited the strongest interactions with both proteins. In summary, this work suggested that NSAIDs, especially ketoprofen and naproxen, have promising potential as candidates for further in vitro and in vivo investigations to inhibit the QS circuits of P. aeruginosa. Manuscript profile -
Open Access Article
15 - Biological Removal of Zinc from Automotive Hazardous Paint Sludge: a comparison between an endogenous Pseudomonas aeruginosa isolateand Acidithiobacillus thiooxidans PTCC1692
Fatemeh Honarjooy Barkusaraey Roya Mafigolami Gholam KhayatiResearch on the hazardous waste of paint sludge is mostly focused on recovering the sludge and pollution control with methods such as solidification. This research aimed to study the comparison of biological removal of zinc from paint sludge using a native Pseudomonas a MoreResearch on the hazardous waste of paint sludge is mostly focused on recovering the sludge and pollution control with methods such as solidification. This research aimed to study the comparison of biological removal of zinc from paint sludge using a native Pseudomonas aeruginosa strain and Acidithiobacillus thiooxidans PTCC 1692. Samples of water-based paint sludge were collected under aseptic conditions. Isolation of endogenous strains was performed in the Bushnell-Haas (BH) medium from paint sludge, and A. thiooxidans PTCC 1692 was cultured in the medium 119. Optimization of the process parameters for the biological removal of zinc was performed using the design of the experimental (DOE) method for both organisms. The results showed that A. thiooxidans is capable of biological removal of zinc with a higher pulp density and with a more extended bioleaching period. The results of the chemical analysis show the removal of zinc metal by 57.76% compared to P. aeruginosa by 36.39%, and, the removal of other metals such as Cr, Ni, Pb, and Cd were observed in the residual sludge from the process. Energy Disperse X-ray analysis (EDX) showed the more favorable effect of this bacterium in reducing heavy metals. Manuscript profile -
Open Access Article
16 - Investigating the Severity of Environmental Stress Conditions on Growth, the Protein Profile and Biochemical Characteristics of Pseudomonas aeruginosa ATCC 27853
Azita Tisheyar Moahmmad Faezi Ghasemi Nour AmirmozafariPseudomonas aeruginosa is the main cause of nosocomial infections, and is resistant to most antibiotics, this study aimed to evaluate the effects of different stress conditions on growth, the protein profile, and biochemical characteristics of this bacterium. The cells MorePseudomonas aeruginosa is the main cause of nosocomial infections, and is resistant to most antibiotics, this study aimed to evaluate the effects of different stress conditions on growth, the protein profile, and biochemical characteristics of this bacterium. The cells of P. aeruginosa ATCC 27853 in the logarithmic phase were exposed to different stress factors such as sucrose concentration, ethanol, acid, osmotic pressure, and CoCl2. Following each stress condition, the growth and the survival of bacterial cells were determined. Microscopic observation showed morphological changes in different stress conditions. P. aeruginosa ATCC 27853 tolerated up to pH 3, 55% (V/V) ethanol, and CoCl2 up to 7% (W/V), and beyond these amounts, the bacterium lost its ability to survive. Maximum tolerance to sucrose was about 35% (W/V). The results showed that different stress conditions could not effect on the main biochemical characteristics of P. aeruginosa ATCC 27853. Scanning electron microscopy of the cells exposed to different stress conditions showed wide changes in the morphology of cells. In addition, upon treatment of different stresses, significant changes were observed in the protein profile of P. aeruginosa ATCC 27853 according to SDS-PAGE analysis. It can be concluded that severe environmental stresses have great effects on the growth pattern, phenotypic characteristics, and protein profile of P. aeruginosa ATCC 27853. If the stresses are induced all at once, they will cause death, but if they are affected slowly and for a longer period, most bacteria will be able to repair the damaged parts, and the growth of will resume. Manuscript profile -
Open Access Article
17 - The Impact of Rhizospheric Pseudomonas aeruginosa on the Growth of Melissa officinalis
Elham Karami Monir Doudi Zahra Rezayatmand Ladan Rahimzadeh TorabiMicrobial communities occupy a significant position in the functioning and productivity of agricultural ecosystems. The taxonomic genus Pseudomonas encompasses a group of bacterial species that can establish mutually beneficial partnerships with different plants. The ob MoreMicrobial communities occupy a significant position in the functioning and productivity of agricultural ecosystems. The taxonomic genus Pseudomonas encompasses a group of bacterial species that can establish mutually beneficial partnerships with different plants. The objective of this study was to ascertain and isolate Pseudomonas strains that were obtained from the rhizosphere soil of M. officinalis. Furthermore, an examination was conducted on the morphological characteristics of M. officinalis that had been treated with standard and rhizospheric Pseudomonas. The experimental treatments consisted of 3 different groups: a rhizosphere Pseudomonas inoculation with a concentration of 106 and 109 CFU /ml, standard Pseudomonas inoculation with a concentration of 106 and 109 CFU /ml, and a control group that did not contain any bacteria. To effectively separate bacteria, specialized culture mediums, such as the King B medium, were employed. The biochemical and molecular results revealed that the isolated strain was Pseudomonas aeruginosa. The standard Pseudomonas treatment with a concentration of 109 CFU/ml exhibited the greatest root length of 24.1 cm, while the treatment lacking the presence of Pseudomonas displayed the lowest root length of 12.9. Treatments with rhizospheric P. aeruginosa showed the greatest values for stem length, as well as root and stem weight. Meanwhile, the control treatment resulted in the lowest measurements for these mentioned characteristics. Manuscript profile -
Open Access Article
18 - Antimicrobial and Anti-biofilm Effect of Samarium Oxide Nanoparticles on Pseudomonas aeruginosa Isolated from Hospital wards
Hossein Zahmatkesh Mirsasan Mirpour Hojjatolah Zamani Behnam RastiThe development of drug resistance, treatment ineffectiveness, and the occurrence of Pseudomonas aeruginosa infections are primarily attributed to biofilm formation and quorum sensing (QS)-dependent virulence factors. This study aimed to synthesize samarium oxide nanopa MoreThe development of drug resistance, treatment ineffectiveness, and the occurrence of Pseudomonas aeruginosa infections are primarily attributed to biofilm formation and quorum sensing (QS)-dependent virulence factors. This study aimed to synthesize samarium oxide nanoparticles (Sm2O3NPs) using curcumin and evaluate their antimicrobial effects against P. aeruginosa isolated from different hospital wards. Based on TEM, XRD, and EDX analysis, the synthesized nanoparticles exhibited a cubic structure with an average size of 32.61 nm and a pure crystalline phase. The Sm2O3NPs exhibited notable antibacterial efficacy against P. aeruginosa strains. Based on the CV staining assay, sub-MIC of Sm2O3NPs inhibited the biofilm formation of P. aeruginosa strains by 16-69%. Additionally, the levels of pyocyanin in the NPs-treatment groups decreased by 29-58% compared to the control group. This research confirms the significant antibacterial potential of Sm2O3NPs, implying their potential utilization as an effective antibacterial agent against P. aeruginosa strains isolated from various hospital wards, after further characterization. Manuscript profile -
Open Access Article
19 - A review of Pseudomonas aeruginosa in the environment: pathogenesis, antibiotic resistance, genetic diversity, and removal ways from water treatment
Ghasem Ghorbani Ebrahim Rahimi Amir ShakerianPseudomonas aeruginosa as a waterborne pathogen is a growing concern to public health sectors. Many sources of environmental water could potentially be acting as a reservoir for potentially pathogenic strains of P. aeruginosa. Due to the fact that P. aeruginosa is an op MorePseudomonas aeruginosa as a waterborne pathogen is a growing concern to public health sectors. Many sources of environmental water could potentially be acting as a reservoir for potentially pathogenic strains of P. aeruginosa. Due to the fact that P. aeruginosa is an opportunistic pathogen and causes serious problems, it seems necessary to know the pathogenesis, antibiotic resistance, genetic diversity and methods of removing it from drinking water. The pathogenesis of P. aeruginosa has been extensively studied and proven to be a multifactorial process, mediated by quorum sensing. P. aeruginosa possess two quorum sensing systems, las and rhl that facilitate cell to cell communication through production of signalling molecules termed autoinducers to target specific receptors for activation. P. aeruginosa is intrinsically resistant to various antibiotics due to a low permeability in the outer membrane, which acts as a selective barrier. However, this bacterium is a highly diverse pathogen that is capable of adaptation to the surrounding environment. When subjected to antibiotic selective pressure, the induced response facilitates bacterial survival and develops antibiotic resistance. literature suggests that the large genome size and genome complexity are responsible for the ability of this bacterium to adapt and thrive in a diverse range of environments. The presence and persistence of these bacteria in environmental waters may pose a great risk to the public health and requires further work to fully characterize and quantify the input of MDR P. aeruginosa strains from the hospitals compared with those originating from the general community or other wastewater related sources. Manuscript profile -
Open Access Article
20 - Prevalence and antimicrobial resistance pattern of Pseudomonas aeruginosa strains isolated from rose water and herbal distillates in Kashan, 2018
Reza sharafati chaleshtori navid Mazroii Arani Elahe Alizadeh Alireza EtemadiPseudomonas aeruginosa (P. aeruginosa) is an opportunistic gram-negative and pathogenic bacterium that can be transmitted through food to humans. Antibiotic-resistant infections of this bacterium are a severe public health issue. This study aimed to determine the preval MorePseudomonas aeruginosa (P. aeruginosa) is an opportunistic gram-negative and pathogenic bacterium that can be transmitted through food to humans. Antibiotic-resistant infections of this bacterium are a severe public health issue. This study aimed to determine the prevalence and antibiotic resistance pattern of P. aeruginosa strains isolated from rose water and herbal distillates in Kashan. In this cross-sectional study, 400 rose water and herbal distillate samples were randomized purchased of local markets in Kashan and evaluated for the occurrence of P. aeruginosa by culturing methods. The obtained isolates were subjected to the disc diffusion antimicrobial susceptibility tests. Out of 400 samples, 16 (4 %) samples were contaminated withP. aeruginosa. The 100 % of isolates were resistant to ticarcillin, ceftazidime, colistin, and gentamicin. Also, the 13 (81.25 %) samples were sensitive to imipenem. The results showed a high occurrence of the antibiotic resistance P. aeruginosa strains isolated from rose water and herbal distillates. Manuscript profile -
Open Access Article
21 - Prevalence, antibiotic resistance and frequency of virulence factors in Pseudomonas aeruginosa strains isolated from raw and frozen meat
Manizhe Rezaloo Abbasali Motalebi Zohreh Mashak Seyed Amir Ali AnvarPseudomonas aeruginosa strains are considered as the main causes of food spoilage and occasionally foodborne diseases. The present study was performed to evaluate the pattern of antibiotic resistance and frequency of virulence factors in Pseudomonas aeruginosa strains i MorePseudomonas aeruginosa strains are considered as the main causes of food spoilage and occasionally foodborne diseases. The present study was performed to evaluate the pattern of antibiotic resistance and frequency of virulence factors in Pseudomonas aeruginosa strains isolated from meat. A total of 120 samples of raw and frozen meat were collected. Samples were evaluated for the presence of Pseudomonas aeruginosa using conventional microbial culture. The pattern of antibiotic resistance of Pseudomonas aeruginosa isolates was evaluated using the disk diffusion method. Genomic DNA was extracted from Pseudomonas aeruginosa isolates and the frequency of virulence factors was assessed using the polymerase chain reaction. Eleven out of 120 meat samples (9.16%) were contaminated with Pseudomonas aeruginosa. The prevalence of Pseudomonas aeruginosa in raw and curled meat samples was 5.00 and 13.33%, respectively. There was a statistically significant difference in the prevalence of Pseudomonas aeruginosa between raw and frozen meat samples (P <0.05). Pseudomonas aeruginosa isolates had the highest rate of antibiotic resistance against ampicillin (100%), penicillin (90.90%) and tetracycline (81.81%). The prevalence of resistance to imipenem (9.09%) and trimethoprim (18.18%) antibiotics was lower than other cases. ExoU (54.54%) and exoT (18.18%) genes were the most frequent virulence factors detected. The simultaneous presence of virulence factors and antibiotic resistance in Pseudomonas aeruginosa strains isolated from raw and frozen meat increases the need for further studies to confirm the role of this bacterium as an important food-spoilage bacterium. Manuscript profile -
Open Access Article
22 - Evaluation of the effect of cold plasma against Pseudomonas aeruginosa inoculated in rose water
Zeinab Rahmani Fatemeh Mohamadi Reza Sharafati ChaleshtoriCold plasma is a novel non-thermal food processing technology that uses reactive medium and energetic photons and particles, to inactivate contaminating microbes on various foods. The aim of this study was to investigate the effect of atmospheric pressure cold plasma on MoreCold plasma is a novel non-thermal food processing technology that uses reactive medium and energetic photons and particles, to inactivate contaminating microbes on various foods. The aim of this study was to investigate the effect of atmospheric pressure cold plasma on Pseudomonas aeruginosa in vitro and rose water. In this experimental study, P. aeruginosa was exposed to 15 kHz plasma for 10, 30, 50 and 70 seconds. The effect of plasma on rose water inoculated with the bacterium at a concentration of 105 CFU / mL during storage for 7 days at room temperature was also investigated. The results showed that the highest non-growth halo diameter in P. aeruginosa was 31 mm in 70 seconds treatment. In addition, the plasma had an inhibitory effect on growth of P. aeruginosa in rose water during storage. Therefore, the use of plasma as a non-thermal and microbial reducing method in the rose industry is recommended. Manuscript profile -
Open Access Article
23 - گلهای رز ایرانی، یک ضد باکتری طبیعی علیه عامل اصلی عفونت سوختگی پوست، سودوموناس آئروژینوزا
مائده رزقی سید رضا حسینی دوست ژینوس عسگرپناهمقدمه و هدف : رز ایرانی (Rosa foetida) متعلق به تیره گل سرخ می باشد و یکی از گونه های بومی ایرانی به حساب می آید که تاکنون اثرات بیولوژیک گل های آن بررسی نگردیده است. از آنجا که گل های این گیاه در طب سنتی ایران به عنوان مرهم جهت بهبود سوختگی های عفونی پوست به کار می رود Moreمقدمه و هدف : رز ایرانی (Rosa foetida) متعلق به تیره گل سرخ می باشد و یکی از گونه های بومی ایرانی به حساب می آید که تاکنون اثرات بیولوژیک گل های آن بررسی نگردیده است. از آنجا که گل های این گیاه در طب سنتی ایران به عنوان مرهم جهت بهبود سوختگی های عفونی پوست به کار می رود، بر آن شدیم تا برای اولین بار اثر ضد میکروبی عصاره های مختلف گل ها را علیه عامل اصلی عفونت های سوختگی، سودوموناس آئروژینوزا بررسی نماییم. روش تحقیق: بررسی اثر ضد میکروب عصاره ها و تعیین حداقل غلظت مهار رشد باکتری به ترتیب با استفاده از روش چاهک پلیت و روش رقت سازی متوالی در میکروپلیت انجام شد. نتایچ و بحث: تمام عصاره های حاصل از گل ها دارای اثر مهاری بر رشد باکتری بودند که از بین آنها عصاره های آبی و متانولی دارای قوی ترین اثر را نشان دادند. قطر هاله عدم رشد (با غلظت 125 میلی گرم/میلی لیتر) و حداقل غلظت مهار رشد باکتری برای هر دو عصاره فوق نسبتا با اثر داروی استاندارد، ایمیپنم/سیلاستاتین (8/8 میکروگرم/میلی لیتر) یکسان بود. توصیه کاربردی / صنعتی: نتایج مشاهده شده نشان می دهد که گل های رز ایرانی دارای ترکیبات موثره با اثر ضد باکتری علیه سوش های استاندارد و پاتوژن سودوموناس آئروژینوزا جدا شده از پنج بیمار مبتلا به عفونت سوختگی پوست است و کاربرد سنتی این گیاه را در عفونت های سوختگی پوست توجیه می نماید. Manuscript profile -
Open Access Article
24 - Investigation of the frequency of exoA and exoT genes in clinical isolates of Pseudomonas aeruginosa and antibiotic resistance of strains
Shadi Mahboubi Somayeh Ataei-e JalisehPseudomonas aeruginosa is a gram-negative bacillus and an opportunistic pathogen. This bacterium is the third most common cause of nosocomial infections. Exoenzymes T, S, U, Y are secreted by the type III secretion system, which is one of the important pathogenic factor MorePseudomonas aeruginosa is a gram-negative bacillus and an opportunistic pathogen. This bacterium is the third most common cause of nosocomial infections. Exoenzymes T, S, U, Y are secreted by the type III secretion system, which is one of the important pathogenic factors of this bacterium, and exotoxin A is secreted by the type II secretion system. In this study, the frequency of exoA and exoT genes in clinical strains of Pseudomonas aeruginosa and the antibiotic resistance of the strains were investigated. 29 strains of P. aeruginosa bacteria were identified from patients admitted to Razi Hospital in Rasht, using common microbiology methods and were examined along with the standard strain of ATCC27853. The resistance of these strains to 6 types of antibiotics was determined using disk diffusion method. Then DNA extraction and PCR was performed to determine the frequency of exoA and exoT genes. In this research, the highest rate of antibiotic resistance was observed for Imipenem(86.75). The results obtained from PCR showed a frequency of 37.93%for exoA and 68.96% for exoT. Manuscript profile -
Open Access Article
25 - Biodesulphurization of dibenzothiophene as a sulphur Model compound in heavy fuel oil by supported bacterial strain on polyethylene
Babak Ghorbani Barnaji Soroor Sadeghi farhad salimiA new biodesulfurization method has been considered using Pseudomonas aeruginosa supported on polyethylene (PE) for biodesulfurization (BDS) of dibenzothiophene (DBT) as heavy fuel oil sulphur compound model. The obtained results according to Spectrophotometric analysis MoreA new biodesulfurization method has been considered using Pseudomonas aeruginosa supported on polyethylene (PE) for biodesulfurization (BDS) of dibenzothiophene (DBT) as heavy fuel oil sulphur compound model. The obtained results according to Spectrophotometric analysis at 325 nm showed that 90.54 % of DBT at the primary concentration about 5 (mg.L-1), pH=7, biocatalyst dosage of 0.1 g, in 37 °C and after 90 min of contact time has been removed. These optimum conditions have been applied for heavy fuel oil (mazut) samples and the biodegradation of their total sulphur content (TSC) has been investigated by X- ray fluorescence spectrometer (XRF). The obtained results revealed that 33.075 % of total sulphur content from mazut sample has been removed. Kinetic study predicted the chemisorption process as the rate determining step, as it followed the pseudo-second-order rate equation. The data for DBT adsorption on biocatalyst fitted to the Freundlich isotherm model. Morphology and surface functional groups of the biocatalyst have been investigated by SEM and FT-IR, respectively. Manuscript profile -
Open Access Article
26 - Quinolone resistance associated with efllux pumps mexAB-oprM in clinical isolates of Pseudomonas aeruginosa
Mitra Salehi Mina Hekmatdoost Farzaneh HosseiniBackground and Objectives: Pseudomonas aeruginosa is one of the most common antibiotic resistant bacteria and, therefore, treatments of Pseudomonad-caused infections are complicated. MexAB-OprM pump exports many antimicrobial compounds regardless of their structural and MoreBackground and Objectives: Pseudomonas aeruginosa is one of the most common antibiotic resistant bacteria and, therefore, treatments of Pseudomonad-caused infections are complicated. MexAB-OprM pump exports many antimicrobial compounds regardless of their structural and functional similarities. The mexB gene encodes a proton-drug antiporter in MexAB-OprM pump. The present study was aimed to evaluate and detect the quinolone resistance associated with efllux pumps mexAB-oprM in P. aeruginosa isolated from clinical specimens. Materials and Methods: This cross-sectional study was performed on 104 P. aeruginosa isolated from patients hospitalized in Trauma and Burn Intensive Care Unit (TBICU). The Genera and strains were identified primarily based on biochemical tests. The evaluation of antibiotic resistance pattern was carried out using disk diffusion method for 11 common antibiotics and microdilution broth method for 4 antibiotics. The presence of mexB gene was investigated using PCR method. Results: According to the results, the most antibiotic resistance pattern were seen through treatment with nalidixic acid (86.54%), ceftazidime (82.2%) and ofloxacin (81.78%). Furthermore, the minimum antibiotic resistance were observed through treatment with imipenem (40.91%), piperacillin (44.9%) and tetracycline (48.03%). Based on MIC, the highest and lowest antibiotic sensitivity was recorded for tetracycline and ceftriaxone, respectively. Based on the PCR method, 27% of the clinical isolates harbor the mexAB-oprM operon. Conclusion: Based on the results, there is a significant association between presence of mexB gene and mexAB-oprM pump and antibiotic resistance in P. aeruginosa. Regarding the importance of antibiotic resistance, the study of other efllux pumps, comparison of antibiotic resistance profile and the relationship between efllux pumps and clinical origin of the strains are recommended. Manuscript profile -
Open Access Article
27 - Determination of antibiotic susceptibility pattern of Pseudomonas aeruginosa and the prevalence of bla OXA-23 gene in isolates from a Hospital in North Khorasan
Mohadeseh Radmehr majid moghbeliBackground & Objectives: Pseudomonas aeruginosa is one of the most important pathogens of opportunistic infections, which is considered as one of the main causes of nosocomial infections. The aim of this study was to isolate pseudomonas aeruginosa and identify strai MoreBackground & Objectives: Pseudomonas aeruginosa is one of the most important pathogens of opportunistic infections, which is considered as one of the main causes of nosocomial infections. The aim of this study was to isolate pseudomonas aeruginosa and identify strains with bla OXA-23 gene using polymerase chain reaction (PCR) technique. Materials & Methods: In this study, 70 isolates of pseudomonas aeruginosa from various infections including lung, urine, blood, wound and sputum were collected in north khorasan hospital, after phenotypic and genotypic confirmation of the isolates, antibiotic discs of doripenem and imipenem (Rosco), meropenem and ciprofloxacin, colistin and amikacin (Mast) were used to perform antibiotic susceptibility testing of bacteria by disk diffusion method. Results: Isolates showed 32.8%, resistance to amikacin , 47.1% todoripenem, 37.1% to imipenem, 40% to ciprofloxacin, and 10% to colistin. The highest resistance of the strains was related to meropenem 61.4% and the lowest was colistin with 10% resistance. Conclusion: In this study, the resistance of pseudomonas aeruginosa to the antibiotic meropenem was 61.4% and compared to other studies, including in ethiopia, showed a higher level of antibiotic resistance, this level of carbapenem resistance indicates an increase in resistance of pseudomonas aeruginosa strains to carbapenems. Manuscript profile -
Open Access Article
28 - Molecular Identification of Native Pseudomonas Strains with Polyethylene Degradation Ability from Soil
Hanieh Shahreza Abbas Akhavan Sepahi farzaneh hosseini Ramezan Ali KhavarinejadBackground & Objectives: Nowadays, high consumption plastics such as polyethylene are recognized as one of the major environmental pollutants by the World Environment Organization. The aim of this study was to isolate MoreBackground & Objectives: Nowadays, high consumption plastics such as polyethylene are recognized as one of the major environmental pollutants by the World Environment Organization. The aim of this study was to isolate and identify native Pseudomonas bacterial strains with the polyethylene packaging degradation ability. Materials & Methods: In order to conduct this study, soil samples were collected. In order to isolate the isolates with polyethylene degradation ability, two methods of direct culture and culture by pre-enrichment method were used. After culturing the bacteria in MSM medium and examining the percentage of plastic weight loss, the superior strain was selected for DNA extraction and PCR of alk-B gene. PCR results were sequenced and examined phylogenetically. Results: The results of the present study showed that the percentage of degradation of polyethylene by Pseudomonas strains was 7.2% at most and 4.5% on average. Also, all purified degrading bacteria in this study harbored alk-B gene. Conclusion: The results showed that the degradation of polyethylene materials can be significantly accelerated by using and optimizing bacteria isolated from soil. It seems that by promoting genetic methods based on the genome of bacteria, especially the strain of Pseudomonas aeruginosa, it is possible to develop methods during which all commonly used types of polyethylene are degraded in a much shorter time than normal. Manuscript profile -
Open Access Article
29 - The evaluation of the effect of gold nanoparticles on the expression of (MexA/B) efflux pump genes from the RND family in multidrug resistant Pseudomonas aeruginosa strains
Nehleh Falahati Houshang Jamali Mohammad Kargar Farshid KafilzadehBackground and purpose: Pseudomonas aeruginosa has several protein pumps with which it puts antibiotics out. This has led to the formation of multidrug resistance. The aim of this study was to investigate the effect of gold nanoparticles on the expression of mexA/B gene MoreBackground and purpose: Pseudomonas aeruginosa has several protein pumps with which it puts antibiotics out. This has led to the formation of multidrug resistance. The aim of this study was to investigate the effect of gold nanoparticles on the expression of mexA/B genes in multidrug resistant Pseudomonas aeruginosa strains. Materials and Methods: In this cross-sectional descriptive study, 74 suspected samples of Pseudomonas genus were collected and phenotypic and genotypic tests were used to screen Pseudomonas aeruginosa.Then, anibiogram test using 11 antibiotics from different classes was performed by the use of CLSI 2020 standard tables and the disk diffusion method.The frequency of mexA/B genes was evaluated by polymerase chain reaction method and after determining the minimum inhibitory concentration of gold nanoparticles, the effect of gold nanoparticles on the expression of mexA/B genes was evaluated using SYBER Green-Real Time PCR technique. Results: In this study (67.57%) 50 isolates of Pseudomonas aeruginosa were identified. The highest and lowest levels of antibiotic resistance were related to azteronam (98%) and cefpime (26%). The prevalence of mexA/B genes was respectively MexA (74%), mexB (70%) and mexA/B (58%) and also 12% lacked both genes. Gold nanoparticles showed growth inhibitory effect at MIC ≥ 50ppm and at 25 ppm concentration with ciprofloxacin, showed inhibitory effect on the expression of mexA/B genes. Conclusion: The results of this study showed that gold nanoparticles can inhibit the expression of mexA and mexB genes in multidrug-resistant Pseudomonas aeruginosa. Due to the fact that gold nanoparticle does not have toxic effects on eukaryotic cells, it can be important in the treatment process of this group of bacteria. Manuscript profile -
Open Access Article
30 - Comparing the effect of metronidazole with pyocyanin pigment extracted from Pseudomonas aeruginosa on Trichomonas vaginalis in-vitro
sara abdizadehjavazm zohreh momeni mona farhadiBackground and Objectives: Pyocyanin is a blue pigment with oxidation and reduction potential and metabolically active, which has medicinal effects on eukaryotic and prokaryotic cells. Trichomonas vaginalis is the causative agent of trichomoniasis, the most important no MoreBackground and Objectives: Pyocyanin is a blue pigment with oxidation and reduction potential and metabolically active, which has medicinal effects on eukaryotic and prokaryotic cells. Trichomonas vaginalis is the causative agent of trichomoniasis, the most important non-viral sexually transmitted disease in the world. The aim of this study was to evaluate the effect of pyocyanin extracted from Pseudomonas aeruginosa on Trichomonas vaginalis and PC12cell line. Materials & Methods: This study was carried out by an interventional method. First, pyocyanin was extracted from Pseudomonas aeruginosa strain RTCC1474 with the help of chloroform. The relative purity of the pigment was determined by thin-layer chromatography, spectrophotometry UV-Vis and FTIR. Its effect in different concentrations were investigated on Trichomonas vaginalis and PC12 cell line. Results: Pyocyanin at a concentration of 10,000µg/ml in 24 hours and concentrations of 5,000 and 2,500µg/ml in 48 hours caused 100% inhibition of the parasite growth. Its IC50 (Inhibitory Concentration of 50%) level in 48 hours was 17.44µg/ml and the CC50 of this pigment on the cell line was 930 µg/ml, so pyocyanin is effective against the Trichomonas vaginalis and its toxicity on the cell line is 53 times higher than of the parasite (SI=53/32). Conclusion: Considering the inhibitory effect of pyocyanin on the growth of Trichomonas vaginalis based on the results, it is possible that with further research on the extraction and purification of this pigment from various isolates of Pseudomonas aeruginosa, and with additional tests in vitro and in vivo, a more accurate judgment regarding the antiparasitic power of this pigment can be expressed. Manuscript profile -
Open Access Article
31 - Phenotypic and genetically evaluation of the prevalence of efflux pumps and antibiotic resistance in clinical isolates of Pseudomonas aeruginosa among burned patients admitted to Ghotbodin Shirazi Hospital
Yahya Dashtizadeh Afagh Moattari Ali akbar GorzinBackground & Objectives: Pseudomonas aeruginosa is an important cause of nosocomial infections in burnt patients. Antibiotic resistance through various mechanisms is one of challenges for treatment of these patients. The mex efflux pumps play a vital role in the dev MoreBackground & Objectives: Pseudomonas aeruginosa is an important cause of nosocomial infections in burnt patients. Antibiotic resistance through various mechanisms is one of challenges for treatment of these patients. The mex efflux pumps play a vital role in the development of multiple resistances to antimicrobial drugs. This study aimed to analyze the prevalence of genes responsible for efflux pumps mexA-B-oprM and to investigate their phenotypes in the isolates. Materials & Methods: In this cross-sectional study, 250 swabs were obtained from wounds of the patients suffering of burns levels 2 and 3 who admitted in Ghotbodin Shirazi hospital, Shiraz. Presence of P. aeruginosa isolates were confirmed by biochemical tests and PCR. Drug susceptibility, the phenotypic activity of efflux pumps and the presence of mex A, mex B genes were determined using disk diffusion method, cartwheel method and PCR methods, respectively. Results: In this study, 26.40% (66 cases) of patients with burn wounds were infected with P. aeruginosa. These bacteria were resistant to all antibiotics tested except for colistin. Totally, 66.66% of the isolates (44 cases) had an efflux pump, among them 42.92% and 87.87% of the isolates carried mexA and mexB genes, respectively. Conclusion: Our finding showed that genotypic method is very accurate and reliable than phenotypic methods for detection of efflux pumps in clinical isolates of P. aeruginosa. Due to presence of the efflux pump genes in more than 90% of the P. aeruginosa isolates, analysis of the presence of these genes is very important for suggestion of an effective treatment model for the patients with bacterial infection. Manuscript profile -
Open Access Article
32 - Immunization of Pseudomonas aeruginosa alginate - conjugated to diphteria toxoid in mice
Ghazaleh Jaberi Reza Shapouri Ashraf KariminikBackground and Objectives: Pseudomonas aeruginosa is one of the most important infectious agent in medicine. These bacteria cause several infections especially in the patient with high degrees of burns and in immunodeficiency cases. The purpose of this study was providi MoreBackground and Objectives: Pseudomonas aeruginosa is one of the most important infectious agent in medicine. These bacteria cause several infections especially in the patient with high degrees of burns and in immunodeficiency cases. The purpose of this study was providing conjugated antigen component that can induct production of antibody and memory immune in mouse model against Pseudomonas aeruginosa. Materials and Methods: After culturing P. aeruginosa strain PAO1 and extraction of alginate, Diphteria toxoid, ADH and EDAC were added as protein carrier, spacer and linker, respectively to the alginate. After passing the Alginate diphtheria toxoid conjugated (ALG-DT) through chromatography column (CL-2B), its quality was checked to get the quality control label. The prepared antigens were intraperitoneally injected to BALB/c mice (10µg/ml/mouse) for three times once each two weeks. Blood sampling was performed after two weeks of each injection and the levels of serum IgG, IgA and IgM were measured by ELISA. Results: The titer of serum antibodies of vaccinated group with ALG-DT increased significantly after each injection. The levels of serum IgG, IgM and IgA against alginate in vaccinated group was significantly more than the control groups, and after third injection reached to 3.5, 1.7 and 1.2 times increases, respectively. Conclusion: The increases in the levels of antibodies in vaccinated groups are an indicator of activation of T-cells and memory cells. As a result, the conjugated alginate-diphtheria toxoid can be appropriate candidates for production of vaccine. Manuscript profile
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