افشین ذاکری Articles List

List of Articles افشین ذاکری

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1 - Comparative study of the effect of Immunowall and Avilamycin on antibody titre against Newcastle B1 vaccine in broiler chickens
افشین Zakeri سعید Charkhkar Sh Zakeri بهرام Reihani

     The purpose of the present study was to comparatively investigate the effect of Avilamycin and Immunowall on the improvement of humoral immunity in boiler chickens. In this study, 360 cobb broiler chickens were divided into three similar groups More

     The purpose of the present study was to comparatively investigate the effect of Avilamycin and Immunowall on the improvement of humoral immunity in boiler chickens. In this study, 360 cobb broiler chickens were divided into three similar groups of 120 chickens with four replicates of 30 chickens in each group. In the first and second experimental groups, 1 kg/ton of Immunowall and Avilamycin respectively were added to the basic diet while in the third control group only the basic diet was used. On days 9, 17 and 25 of the breeding period (corresponding to 1 day before and 7 and 14 days after the first Newcastle B1 vaccination), 40 chickens were randomly chosen in each experimental group, their blood samples collected, and then serum antibody titres of these chickens against the Newcastle vaccine were measured by HI test. After stastistical analysis using ANOVA and Duncans’s multiple range test, the results of serum antibody titres by HI test on day 9 of the breeding period was not statistically significant. The results of HI test on day 17 of the breeding period (5.01 ± 0.32, 5.27 ± 0.27 and 5.13 ± 0.20 in the control and experimental groups 1 and 2 respectively) was also statistically not significant. The HI test results on day 25 of the breeding period (5.04 ± 0.44, 5.87 ± 0.47 and 5.61 ± 0.41 in the control and experimental groups 1 and 2 respectively) indicated significant statistical differences (P<0.05) between experimental groups 1 and 2 and the conrol group. However, there were no significant differences between the two experimental groups although serum antibody titres in experimental group 1 (Immunowall) showed 0.26 log increase compared to experimental group 2 (Avilamycin). Immunowall as a natural product is notably capable of improving anti B1 antibody production in response to Newcastle B1 vaccine and as it does not leave any drug residues in poultry meat, it can be a suitable alternative to growth promoting generation of antibiotics.   Manuscript profile
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2 - Comparison of serum antibody titer of 8 types of commercial live infectious bronchitis vaccine in broiler chickens
saman mahdavi afshin zakeri yuosef Mehmannavaz

The aim of this study was to compare the serum antibody titer of 8 types of commercial live infectious bronchitis vaccines in broiler chickens. Three hundred and twenty one-day-old male broiler chickens (308 Ross strain) were divided into 8 groups (each group comprised More

The aim of this study was to compare the serum antibody titer of 8 types of commercial live infectious bronchitis vaccines in broiler chickens. Three hundred and twenty one-day-old male broiler chickens (308 Ross strain) were divided into 8 groups (each group comprised of 3 replicates) randomly. At 8 days of age, each treatment group received different types of live infectious bronchitis vaccine by eye-drop method. Twelve birds from each group were randomly chosen and blood sample was taken on the 22nd and 29th days of experimental period and ELISA test was performed on sera for detection of sample antibody titer. The mean of serum antibody titer against infectious bronchitis vaccines among treatment groups on the 22nd day of experimental period showed statistical significance between the group that had received HIPRAVIAR Colon/ H120 and the groups that had received Nobilis Ma5+ Clone30, H120 and Cevac Vitabron L (p<0.05) but no statistical significance was seen between the other treatment groups. The mean of serum antibody titer against infectious bronchitis vaccines among treatment groups on the 29th day of experimental period showed no statistical significance between the treatment groups. The comparison of serum antibody titer against infectious bronchitis vaccines in treatment groups between the 22nd and 29th day of experimental period showed statistical significance between the groups that had received Gallivax IB88, Nobilis Ma5+Clone30 and Cevac Vitabron L (p<0.05) but there were no statistical significance between the other treatment groups.  Manuscript profile
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3 - Detection of Mycoplasma gallisepticum by 16S rRNA PCR with specific primers in clinical samples
S.A Pourbakhsh افشین Zakeri نریمان Sheikhi سعید Charkhkar عباس Ashtari

Mycoplasma gallisepticum (MG) as one the major pathogens of birds, causes significant economic losses in poultry industry. The main purpose of the present study was to detect Mycoplasma gallisepticum in clinical samples using the 16S rRNA PCR method. For serological scr More

Mycoplasma gallisepticum (MG) as one the major pathogens of birds, causes significant economic losses in poultry industry. The main purpose of the present study was to detect Mycoplasma gallisepticum in clinical samples using the 16S rRNA PCR method. For serological screaming test, 18 commercial laying farms and 8 broiler breeder farms were selected and rapid serum agglutination test (RSAT) was performed. For polymerase chain reaction sampling, 10 of the 17 farms that were positive in RSAT were selected and 109 sterile swab samples were collected from the palatine cleft, trachea, air sacs and lungs in each farm. Three swabs from three birds were placed in test tube containing 1 ml of phosphate buffered saline and transferred to laboratory form PCR testing. In this study, specific primers for 16S rRNA gene were used. The aforementioned primers are totally specific for MG and can be differentiated from other Mycoplasmaand bacteria present in the trachea of poultry of the 26 farms examined, 17 farms were positive in RSAT serologic test. The 530 bp PCR product produced by specific primers of all field strains appeared on electrophoresis gel in 46 samples from 10 farms accounting to 42.2%.The 16S rRNA PCR with very high sensitivity can be employed in definitive diagnosis of Mycoplasma gallisepticum infection in vitro.   Manuscript profile
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4 - The comparative effect of prebiotic, growth promoter antibiotic, probiotic, yeast cell wall and acid fire on broiler chickens performance
افشین Zakeri مهدی Taghi Nejad Roudbaneh آیدین Azizpour وحید Hajiabaloo

In this study 600 Cobb 500 broiler chickens were divided in six similar groups (A, B, C, D, E&F) with 100 chickens in each group (with four replicates of 25 chickens in each group). 100 g/ton growth promoter antibiotic in experimental group (B), 100 g/ton Prebiotic More

In this study 600 Cobb 500 broiler chickens were divided in six similar groups (A, B, C, D, E&F) with 100 chickens in each group (with four replicates of 25 chickens in each group). 100 g/ton growth promoter antibiotic in experimental group (B), 100 g/ton Prebiotic in experimental group (C), 800 g/ton Acid fires in experimental group (D), 1 kg/ton MOS in experimental group (E) and 4 kg/ton Yeast cell wall in experimental group (F) were added to the basic diets however the control group (A) chickens were fed only with the basic diet. Every week 100 chickens from each group were selected randomly and productive parameters such as weight gain, FCR, EEF, mortality, feed intake (FI) were calculated. On days 9, 17 and 25 of growth (1 day before and 7, 14 days after first Newcastle B1 vaccination), from each groups, each time 20 chickens were chosen randomly and serum antibody titres of these chickens were measured against Newcastle vaccine by HI test. The results obtained from statistical analysis indicated that using natural growth promoting substances not only increases humoral immunity but it also leads to improvement of productive parameters (p<0.05).   Manuscript profile
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5 - The effect of yeast β-glucan on the amount of albumin, globulin, urea and total protein of broiler chickens
ali kargarirezapour somayye ghobadi afshin zakeri

   Glucans derived from yeast cell wall are promising alternatives to antibiotics, as they have been shown to improve growth performance and stimulate the immune system of immature broilers. In this study we evaluated the effect of different levels of yeast be More

   Glucans derived from yeast cell wall are promising alternatives to antibiotics, as they have been shown to improve growth performance and stimulate the immune system of immature broilers. In this study we evaluated the effect of different levels of yeast beta-glucan (YBG) on some blood parametrs of broiler chickens. In a factorial experiment based on completely randomized design (the first factor: YBG levels: 0, 0.04 and 0.08% of basal diet and sex as a second factor) 144 day old chicks (72 male and 72 female) were selected and allocated to different treatments (three replicates of each treatment). The overall experimental period was 34 days. At the end of study, two birds from each pen were randomly selected as a sample. The level of albumin, globulin, urea and total protein was measured on blood samples. Statistical analysis of the results showed that the YBG had no significant effect on albumin, globulin, urea and total protein level. But the amount of plasma albumin and total protein in female chicks was significantly higher than male chicks (p<0.05). The interaction effect of the two factors was not significant. Therefore, it can be concluded that the mentioned levels of yeast beta-glucan had no significant effect on the studied parameters. Manuscript profile

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