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      • Open Access Article

        1 - The effects of irrigation periods and soil texture on fruits number, vascular transport defect of ovule (seed) and fruits blankness in two pistachio (Pistacia vera L.) cultivars
        farkhondeh rezanejad Najmeh Hosseini Elaheh ZamaniBahramabadi
        The vascular system allows plants to rapidly transport water and nutrients over long distances between roots and other plant parts. It also provides mechanical support for the plant body. In this study, the effect of soil texture (light and heavy) and irrigation periods More
        The vascular system allows plants to rapidly transport water and nutrients over long distances between roots and other plant parts. It also provides mechanical support for the plant body. In this study, the effect of soil texture (light and heavy) and irrigation periods (24 and 48 days) were assayed on vascular transport defect of ovules and fruits blankness in two pistachio cultivars (Kalehghoochi and Ahmadaghae). Eight weeks after flowering, some branches were kept in disodium fluorescein solution. Then, the small fruits were sectioned by hand and studied with fluorescence microscopy for vascular defect. The rate of fruit blankness was determined at harvest time too. Overall levels of developed ovules (fruits) in each inflorescence as well as fruit blankness were studied. The fruits numbers were higher in shorter irrigation period (24 days) and heavy soil compared with longer irrigation period (48 days) and light soil. Also, the rate of vascular transport defect in ovules as well as fruit blankness were higher too. These results can be attributed to the higher number of fruits produced in high water availability and heavy soil, as optimal condition, and in results, their stronger competition for fruit filling. It was also shown that in comparison with Ahmadaghaee cultivar, the Kaleghoochi cultivar mostly through the vascular transport blocking in developing ovules (seeds) is resulted in blankness of fruits. Vascular transport defects can occur transiently and be repaired by the plant. Manuscript profile
      • Open Access Article

        2 - Identification, sequencing and phylogeny of APETALA1 ortholog gene (AP1) in Eruca sativa (Brassicaceae)
        فرخنده رضانژاد Elahe Abulhassani Mahbubeh Sheikh bahaei
        The transition from vegetative to reproductive phase is an important developmental under genetic control. This stage requires the activation of a set of genes in apical meristem, the expression of which transforms the meristem from vegetative to reproductive. The APETAL More
        The transition from vegetative to reproductive phase is an important developmental under genetic control. This stage requires the activation of a set of genes in apical meristem, the expression of which transforms the meristem from vegetative to reproductive. The APETALA1 (AP1) gene plays an important role in transition phase and flower meristem identity. The identification and homology of this gene and its deduced protein in Eruca sativa was studied. Total RNA was extracted from flower buds and cDNA made. Specific primers were designed and then used for RT-PCR reaction. The results showed that the desired fragment of the gene contains 782 nucleotides (complete cds). This fragment was called EvsAP1 and recorded in the NCBI database (KX524132.1). BLAST of this sequence with other species, showed that Raphanus sativus and Brassica nigra have the highest similarity (87%) with EvsAP1. The deduced protein of the EvsAP1 gene contains 256 amino acids, which is 82% similar to Raphanus sativus. The total length of AP1 protein obtained from NCBI databases in most species of Brassicace is 256 amino acids confirming the sequence. Examination of location of the different species in the phylogenetic tree showed that all species of Brassicaceae place in clads close to Eruca sativa. The species has the highest similarity with Brassica nigra and Raphanus sativus so that the later and Eruca sativa located in one clade. These results confirm the accuracy of the obtained sequence for the AP1 gene in this specie. Manuscript profile
      • Open Access Article

        3 - Morphology, ecology and propagation of endangered species of Tecomella undulata (Sm.) Seem )Bignoniaceae) in Golparaki plain of Jiroft (Iran)
        Farkhondeh Rezanejad Asma Saberi Fatemeh Alimoradi
        Tecomella undulata (Sm.) Seem. is deciduous or nearly evergreen tree that is highly valuable due to showy and beautiful flowers, resistance against extreme temperature and drought, medicinal properties, wood production and ... Morphology, ecology and propagation of this More
        Tecomella undulata (Sm.) Seem. is deciduous or nearly evergreen tree that is highly valuable due to showy and beautiful flowers, resistance against extreme temperature and drought, medicinal properties, wood production and ... Morphology, ecology and propagation of this species in Golparaky plain of Jiroft was studied. The highest of its distribution in middle east is seen in this plain that has been introduced as genetic reservoir. The species grows on sand dunes and sand loams or stony places of river bed. pH of area soil is equal 7.5 and its EC is 1/3 desi zimen m -2. The amount of micro- and macro-elements and organic carbon of soil was lower than standard. Root branches are long and various which produce new plantlets using vegetative propagation even in distances of 30 m farther. The inflorescence is raceme-cyme, flowers are odorless, protoandrous and contain heteromorph stamens and adnate perianth. In the middle of flower is seen a well-developed nectary disc producing about 1 ml nectar of odorless or purple color. Flowers are cross pollinated but no pollinator was observed in pollination season. The species is fruitless and seedless although flowers seemed normal. Root branches endanger due to floods and soils of sandy and loamy. The seed absence, less vegetative propagation and losing of vegetatively propagated seedlings due to grazing by cattle and sheep and increasing dry and high increase its treat risk. Thus, its protection should be taken seriously. Also, it is recommended to consider to its vegetative reproduction. Manuscript profile
      • Open Access Article

        4 - The survey of synorganization of floral parts and pollinium and pollen germination and tube growth in Calotropis procera (Asclepiadoideae)
        Fatemeh Alimoradi فرخنده رضانژاد
        Calotropis procera (subfamily Asclepiadoideae) is one of important medicinal plants in desert and natural resources that has wide distribution in dry and hot areas of southern of Kerman province, Iran. Synorganization of flower is extreme in Asclepiadoideae among angios More
        Calotropis procera (subfamily Asclepiadoideae) is one of important medicinal plants in desert and natural resources that has wide distribution in dry and hot areas of southern of Kerman province, Iran. Synorganization of flower is extreme in Asclepiadoideae among angiosperms. Synorganization of flower parts and pollen germination and tube growth were studied. The species contains pentamerous, actinomorphic and bisexual flowers with thick, hairy and white-pink calyx. Corolla is saucer shaped and white-violet that are fused in floral bud and its top part reopened at anthesis. Stamens are attached together through stamen tube and pollinia. Anthers are attached to the stigma to form gynostegium and filaments form a tube around the gynoecium. Stamen tube contains guide rail and corona for pollinator attraction and movement. Each anther contains two thecae, each one has one pollinium (pollen sac) in which pollen grains are united as compact in collective envelope. Two pollinia, one each from two neighboring anthers are connected together by a translator (consists of a clip and two arms.) and form pollinarium apparatus. Thus, pollen grains are not dispersed singly; pollinia are always transported together. The translator does not consist of cellular structure. The postgenital fusion occurs between the anthers and pentagonal stigma and in the upper zone between the two carpels. Synorganisation of flower parts is mostly postgenital. In vitro pollen germination and tube growth was high on basal medium at 300C. One or both pollinia from pollinarium apparatus record pollen germination and tube growth. Pollinia growth showed the unilateral organization. Manuscript profile
      • Open Access Article

        5 - Study of some pollen genotypes viability and comparison of natural and manual pollination in pistachio (Pistacia vera L.)
        Najmeh Hosseini فرخنده رضانژاد Elaheh ZamaniBahramabadi
        In plants for more grain production, it is possible to use manual pollination in the absence of enough natural pollination. In pistachio, manual pollination can be a solution to the problem of yield reduction caused by pollen shortage. Here, the viability of several mal More
        In plants for more grain production, it is possible to use manual pollination in the absence of enough natural pollination. In pistachio, manual pollination can be a solution to the problem of yield reduction caused by pollen shortage. Here, the viability of several male genotypes and the effectiveness of manual and natural pollination were compared. 21 male genotypes were selected and the pollen size and tube growth were compared in a completely randomized block design. After 2.5 hours of culture, genotypes 13 (53.33%) and 17 (52.56%) had the highest germination rate and genotype 6 (110 μm) showed the highest pollen tube length. After 5 hours, genotype 21 (74.66%) had the highest germination rate and genotype 8 (150 μm) showed the highest tube length. Manual pollination with a mixture of pollen of high quality genotypes (21, 17, 13, 8 and 6) was performed on two female cultivars of Ahmadaghaei and Kaleghoochi and compared with natural pollination as a split plot design. In manual pollination all flowers had pollen, but in natural pollination, 67% of the flowers were free of pollen. The percentage of falling flowers and fruits in natural pollination (85%) was higher than manual pollination (79%) but, the percentage of fruit blankness in manual pollination (20%) was higher than natural pollination (% 1). It is possible that the high pollen density in manual pollination reduced the flowers drop and increased the number of fruits but, due to insufficiency of nutrients, many seeds could not grow, resulting in blank fruit production. Manuscript profile
      • Open Access Article

        6 - The studies of different culture methods in ors (Juniperus seravshanica)
        Farkhondeh Rezanejad Farzaneh Farzan Elaheh ZamaniBahramabadi Farzad Ganjalikhani hakemi
        Juniperus is the second most prevalent group of conifers on Earth. The reduction in viable seeds and natural regeneration is an important problem of Juniperus. Juniperus seravschanica is important in soil protection and is very resistant to frost and drought too. In thi More
        Juniperus is the second most prevalent group of conifers on Earth. The reduction in viable seeds and natural regeneration is an important problem of Juniperus. Juniperus seravschanica is important in soil protection and is very resistant to frost and drought too. In this study, seed number in cone, seed emptiness and germination were studied on filter paper, peatmoss+ perlite mixture and in field in Galuchar, sarbijan and Dalfard (Kerman province) in populations. In addition, shoot proliferation and rooting in pot and in vitro culture was assayed. The average number of cone seeds in Glochar, Serbijan and Delfard was 4.86, 3.69 and 3.21, respectively. The average number of full seeds per cone was 1.1, 0.22 and 0.1 (22, 5.96 and 3.1%, respectively) in Glochar, Sarbijan and Dalfard, showing 78, 94 and 97% emptiness, respectively. In all media, seed germination percentage was very low (2-5%). No germination was observed in seeds without cold treatment as well as without scarification (making a hole in seed coat). 20 cm young shoots, after cold treatment and rapid immersion in IBA, grew as normal until 4 week and finally dried after 5-6 weeks. The young shoots (1-1.5 cm) cultured in MS and WPM media proliferated and regenerated new branches in WPM (40% regeneration) and MS (57%) media in combination of BAP and NAA (2+3 and 0.2+3 mgl-1, respectively for WPM and MS). No rooting was observed using IBA after 24 weeks. Manuscript profile
      • Open Access Article

        7 - Inflorescence and fruit structure in Maclura pomifera (Moraceae): study of its parthenogenesis
        Roza Arab Farkhondeh Rezanejad
        Maclura pomifera (Moraceae), known as souda or American mulberry, is dioecious, about 5-8 meters long that its fruits have a very sticky sap. In different areas of Kerman city, no male tree was observed and all distributed plants were female. Interestingly, these female More
        Maclura pomifera (Moraceae), known as souda or American mulberry, is dioecious, about 5-8 meters long that its fruits have a very sticky sap. In different areas of Kerman city, no male tree was observed and all distributed plants were female. Interestingly, these female trees, produced seeds. It has been reported that some species Moraceae produce fruits and seeds without fertilization, a phenomenon called parthenogenesis. In this study, the structure of inflorescences and flowers were investigated. Further, some flowers were covered with wax paper bags to prevent pollination. The presence of pollen in pistil tissues was studied with a fluorescent microscopy. The dense and spherical female inflorescence has about 60-70 flowers with a single- whorled perianth similar to the sepals. The sepals were tetrameric and pink at young first and become fleshy and green during growth. The pistil and perianth (sepals) surrounding it together developed to a drupe like fruit. The pistil was unicarpellate and long style. The drupe-like fruits form each inflorescence formed accessory fruit, atypical fruit, with pomaceous view. No pollen grains were observed on pistil tissues (stigma, style and ovary) by aniline blue staining. The fruits of both closed (by wax paper bags) and open inflorescences produced about 8-18 seeds. The seeds was viable and during germination produced seedlings in perlite media without any chemical or physical treatment. Seed formation without pollination and fertilization in this species confirms the parthenogenesis phenomenon; this study is the first report about the occurrence of parthenogenesis in M. pomifera. Manuscript profile