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        1 - Effects of jasmonic acid and titanium dioxide nanoparticles on process of changes of phytochemical and antioxidant in genotypes of Satureja hortensis L.
        Azar Davari Mamoud Solouki Bahman Fazeli-Nasab
        In this research to evaluate the effect of sprying of titanium dioxide nanoparticles (0, 30, 60 and 90 mg/l) and jasmonic acid (0, 50, 100 and 150 mM) on enzymatic and non-enzymatic antioxidants As well as physiological traits 5 genotype of Satureja hortensis L. factori More
        In this research to evaluate the effect of sprying of titanium dioxide nanoparticles (0, 30, 60 and 90 mg/l) and jasmonic acid (0, 50, 100 and 150 mM) on enzymatic and non-enzymatic antioxidants As well as physiological traits 5 genotype of Satureja hortensis L. factorial experiment in a completely randomized design with three replications. Treatments were conducted at four leaf stage on the aerial parts of the plants and then were harvested 48 hours after spraying. At first were extracted from shoots and then all traits; enzymatic and non-enzymatic antioxidants and physiological traits were measured by using a spectrophotometer. Analysis of variance showed that the interaction of spraying different concentrations of titanium dioxide nanoparticles and jasmonic acid, catalase, peroxidase, polyphenol oxidase, ascorbate peroxidase, total protein, flavonoids, anthocyanins and total phenolics were significant at the 1% level. The highest catalase (0.0536 mg per gram of fresh weight) at doses of 30 mg per liter in Tabriz plant genotype nanoparticles of titanium dioxide and 150 mMjasmonic acid per liter;Most of ascorbate peroxidase (0.0514 mg per gram of fresh weight) in plant genotypes in concentrations of 60 mg per liter Ardestān nanoparticles of titanium dioxide and 50 mMjasmonic acid;Most peroxidase activity (0.000494 mg per gram of fresh weight) at a concentration of 60 mg per liter in genotype Savory Ardestān nanoparticles of titanium dioxide and 150 mMjasmonic acid; The highest polyphenol oxidase (0.0628 mg per gram of fresh weight) in Tabriz genotypes in treatments of 30 mg of nanoparticles of titanium dioxide and zero (control) M jasmonic acid was obtained.Savory population most effective physiological traits, Savory Rey, the antioxidant enzyme, Savory Ardestan and Tabriz and the non-enzymatic antioxidants (anthocyanins, phenols and flavonoids) Savory Mashhad. The results of this study showed that nano-titanium and jasmonic caused time and non-enzymatic antioxidant and physiological Savory is the most effective hormone levels nanoparticles of titanium, 30 ppm and jasmonic acid was 50 micro molar. Manuscript profile
      • Open Access Article

        2 - Evaluation of total phenolic, flavenoeid content and antioxidant activity of Leaf and Fruit in 14 different genotypes of Ziziphus mauritiana L. in south of Iran
        Bahman Fazeli nasab Alireza Sirousmehr Naser Mirzaei Morad Solimani
        Medicinal plants uses and their active ingredients as natural anti microbial and antioxidant agents are interested recently, because they had high quality of flavonoid and terpenoid antioxidants get a great importance in the food and drug industries. In this research to More
        Medicinal plants uses and their active ingredients as natural anti microbial and antioxidant agents are interested recently, because they had high quality of flavonoid and terpenoid antioxidants get a great importance in the food and drug industries. In this research to purpose of phytochemical and antioxidant activity of Ziziphus mauritiana L. the leaves and fruit of 14 wild-growing genotypes of plant were collected in 2016 from the southern regions of Iran (Jiroft, Rodan, Minab and Jask). Plant hydro alcoholic extract was obtained by maceration technique. Total phenol  and flavonoid content were measured by Folin-Ciocalteu reagent and  Aluminium chloride method, respectively  and antioxidant activity was evaluated  by DPPH method.The results were showed that the highest total phenol content (20.41 mg GAE / g dry extract) and the flavonoids (52.12 mgQEE / g dry extract) was belong to plant genotype of Rodan region (two).  According to data the leaf extract of plant had more total phenol content and antioxidant activity with IC50= 88.08 µg/ml than fruit extract of plant especially in Rodan region (two). The plant organ and the habitat condition could have a significant effect on the phytochemical content and antioxidant activity of plant extract. So due to high contents of phenol and flavonoid contents and optimal antioxidant activity of plant in Rodan and then Talar region respectively, Ziziphus mauritiana L. could be referred as an natural effective antioxidant get use a great importance in the food and drug industries. Manuscript profile
      • Open Access Article

        3 - Optimization callus indication and Study of FLS and CHI gene expression in quercetin biosynthesis pathway in callus culture with UV-B radiation of Momordica charantia
        ziba fooladvand بهمن فاضلی نسب
        The present study aimed to optimize the callus induction and to determine the expression of flavonol synthase and chalcone isomerase genes in the pathway of quercetin production under UV-B rays in bitter cucumber callus cultivation. In this research was used concentrati More
        The present study aimed to optimize the callus induction and to determine the expression of flavonol synthase and chalcone isomerase genes in the pathway of quercetin production under UV-B rays in bitter cucumber callus cultivation. In this research was used concentration and combine of plant hormone 2, 4-D and BAP and explant to callus induction in Momordica charantia using complete random design with three replication. Genes expression study of Quercetin biosynthesis pathway such as FLS, CHI (were analyzed by 2-∆∆CT) and antioxidant activity assay of phenylalanine ammonia-lyase (Produced by concentration of cinnamic acid) and flavonoid using best callus were selected and affected by UV-B ray (five group(Control(natural light), 5 minute under UV-B ray(studying of gene expression after 24 and 48 hour) and 10 minute under UV-B (studying of gene expression after 24 and 48 hour)). Analysis of variance was shown that kind and concentration of plant hormone was significant on callus induction. Mean square of data was shown that the most callus dry weight was obtained from 0.5 mg/L 2,4-D and 1 mg/L BAP and stem explant. The highest expression of flavonol synthase and chalcone isomerase (chi) was observed in the 10-min UV-B treatment at 24 h after treatment but decreased at 48 hours after treatment. The highest expression of PAL, quercetin and flavonoid were obtained at 5 minutes UV-B treatment at 48 hours after treatment. Overall, the results showed that UV-B radiation at the callus level had a positive effect on the expression of flavonoids biosynthetic pathway genes. Manuscript profile