Effects of jasmonic acid and titanium dioxide nanoparticles on process of changes of phytochemical and antioxidant in genotypes of Satureja hortensis L.
Subject Areas : PhytochemistryAzar Davari 1 , Mamoud Solouki 2 , Bahman Fazeli-Nasab 3
1 - M.Sc. of Plant Breeding, Graduated from University of Zabol, Zabol, Iran
2 - 2Associate Professor, Dept. of Breeding and Biotechnology, University of Zabol, Zabol, Iran
3 - 3Lecturer, Research Dept. of Agronomy and Plant Breeding, Agricultural Research
Institute, University of Zabol, Zabol, Iran
Keywords: Spraying, antioxidants, Jasmonic acid, Satureja hortensis L, Titanium dioxide nanoparticles,
Abstract :
In this research to evaluate the effect of sprying of titanium dioxide nanoparticles (0, 30, 60 and 90 mg/l) and jasmonic acid (0, 50, 100 and 150 mM) on enzymatic and non-enzymatic antioxidants As well as physiological traits 5 genotype of Satureja hortensis L. factorial experiment in a completely randomized design with three replications. Treatments were conducted at four leaf stage on the aerial parts of the plants and then were harvested 48 hours after spraying. At first were extracted from shoots and then all traits; enzymatic and non-enzymatic antioxidants and physiological traits were measured by using a spectrophotometer. Analysis of variance showed that the interaction of spraying different concentrations of titanium dioxide nanoparticles and jasmonic acid, catalase, peroxidase, polyphenol oxidase, ascorbate peroxidase, total protein, flavonoids, anthocyanins and total phenolics were significant at the 1% level. The highest catalase (0.0536 mg per gram of fresh weight) at doses of 30 mg per liter in Tabriz plant genotype nanoparticles of titanium dioxide and 150 mMjasmonic acid per liter;Most of ascorbate peroxidase (0.0514 mg per gram of fresh weight) in plant genotypes in concentrations of 60 mg per liter Ardestān nanoparticles of titanium dioxide and 50 mMjasmonic acid;Most peroxidase activity (0.000494 mg per gram of fresh weight) at a concentration of 60 mg per liter in genotype Savory Ardestān nanoparticles of titanium dioxide and 150 mMjasmonic acid; The highest polyphenol oxidase (0.0628 mg per gram of fresh weight) in Tabriz genotypes in treatments of 30 mg of nanoparticles of titanium dioxide and zero (control) M jasmonic acid was obtained.Savory population most effective physiological traits, Savory Rey, the antioxidant enzyme, Savory Ardestan and Tabriz and the non-enzymatic antioxidants (anthocyanins, phenols and flavonoids) Savory Mashhad. The results of this study showed that nano-titanium and jasmonic caused time and non-enzymatic antioxidant and physiological Savory is the most effective hormone levels nanoparticles of titanium, 30 ppm and jasmonic acid was 50 micro molar.
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