In this split plot study, the morphological and phytochemical changes of Iranian Salvia (Salvia mirzayanii L.) under drought stress and cerium oxide nanoparticle treatment in a randomized complete block design with three replications were investigated. The main plot inc
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In this split plot study, the morphological and phytochemical changes of Iranian Salvia (Salvia mirzayanii L.) under drought stress and cerium oxide nanoparticle treatment in a randomized complete block design with three replications were investigated. The main plot included drought stress at 4 levels of 25, 50, 75 and 100 (control) percentage of the field capacity (FC). The sub-plot was foliar spray of Cerium oxide nanoparticle at five levels of non-consumption (control), 125, 250, 500 and 1000 ml/l. Essential oils were extracted from the organs using water distillation method and the compounds were separated and identified using GC/MS. The results showed that the interaction effect of conditions without drought stress × 1000 ml of nanocerium oxide compared to the control treatment had the greatest effect on increasing plant height (35%), root length (22%), root dry weight (14.9%), essential oil (19%), chlorophyll a (32%), chlorophyll b (31%) and catalase (16%). In addition, drought stress at the level of 25% in 125 ml nano cerium oxide decreased plant dry weight (12%), root length (13%), root dry weight (19%), essential oil percentage (10%), and chlorophyll a (14%). Based on the GS/MS results, decane compound (19.53%) in the condition without drought stress, the compounds of spatholeol (35.86%), docane (18.4%), butanic acid (19.8%) and linalool (25.03%) under condition of stress dryness of 50% field capacity and octane (13.76%) were included. Therefore, the concentration of 1000 mg/liter of cerium nanooxide had the greatest effect on improving the morphophysiological characteristics of Salvia. However, in the condition of 50% field capacity, the secondary compounds of spatholeol, dodecane, butanic acid and linalool were the highest, but in the condition of 75% of the field capacity, the highest amount belonged to the secondary compounds of octane, trimethyl and hexylisovalerate.
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