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        1 - Study the effect of intercropping and chemical fertilizers on essential oil, phenolic and flavonoid contents and some biological properties of hyssop (Hyssopus officinalis L.)
        Farhad Habibzadeh Behvar Asghari
        In order to reduce the use of chemical fertilizers in hyssop (Hyssopus officinalis L.) production, an investigation was conducted in the research farm of Imam Khomeini International University, Qazvin, Iran, in 2016. The study was carried out as a factorial experiment b More
        In order to reduce the use of chemical fertilizers in hyssop (Hyssopus officinalis L.) production, an investigation was conducted in the research farm of Imam Khomeini International University, Qazvin, Iran, in 2016. The study was carried out as a factorial experiment based on randomized complete block design with two factors and three replications. The experimental factors consisted of different planting patterns (factor A) on two levels including sole culture of hyssop and additive intercropping of 100% hyssop + 50% lentil and application of chemical fertilizers (factor B) on two levels including non-application and application of NPK. Essential oil (distillation with clevenger), nitrogen, phosphorus and potassium contents, extract of plant (maceration method), phenolic and flavonoid contents (colorimetric method), DPPH radical scavenging and total antioxidant activity of vegetative parts of the plant at the beginning of flowering stage were evaluated. The results exhibited that all the traits, except for extract content, were significantly affected by the treatments. Oil yield, phenolic and flavonoid content, DPPH radical scavenging activity and total antioxidant activity were significantly stronger in intercropping + fertilizer application compared to the other treatments. In the other hand, intercropping enhanced the phenolic and flavonoid compounds, essential oil and extract yield of hyssop. Secondary metabolites, such as phenolic, flavonoid, and essential oil, are the defense system of plant, which were increased by competition between hyssop and lentil (as a stressor). Antioxidant properties also increased due to the increase in phenolic and flavonoids content. Manuscript profile
      • Open Access Article

        2 - Evaluation of phytochemical, antioxidant and enzymatic inhibitory content of extracts obtained with different solvents from Salvia hypoleuca Benth.
        Behvar Asghari Sudabeh Mafakheri Majid Ghorbani Nohooji
        Various species of Salvia has been used in the pharmaceutical, food and cosmetic industries.In this study, aerial parts of Salvia hypoleuca L. which is an endemic plant to Iran were collected during the flowering stage in the summer of 2020 from Alamut city of Qazvin pr More
        Various species of Salvia has been used in the pharmaceutical, food and cosmetic industries.In this study, aerial parts of Salvia hypoleuca L. which is an endemic plant to Iran were collected during the flowering stage in the summer of 2020 from Alamut city of Qazvin province. In the present study, the total content of phenolic, flavonoid, saponin and tannins, four hexane, ethyl acetate, methanolic and aqueous extracts prepared by maceration method were investigated. The antioxidant properties of these extracts were measured by DPPH, ABTS, phosphomolybdenum and iron chelating methods. In addition, α-amylase, α-glucosidase and tyrosinase enzyme inhibitory effects of the plant were measured by spectrophotometry. Based on the results, ethyl acetate extract of the plant had the highest phenolic and saponin content with values ​​of 87.07 mg gallic acid g-1 and 163.84 mg quillaja saponin g-1, respectively. The aqueous extract of the plant exhibited the highest amounts of flavonoids and condensed tannins content with 33.97 and 5.34 mg quercetin and catechin g-1 in the extract, respectively. Ethyl acetate extract indicated the highest ABTS radical scavenging, total antioxidant, ferrous ion chelating activities, as well as inhibitory effects of α-amylase, α-glucosidase and tyrosinase inhibition properties. The high potential of ethyl acetate extract in biological properties, like antioxidant and antiradical activities could be attributed to its high metabolite contents especially, phenolic and saponin compounds. Thus, it could be concluded that this plant is a rich source of functional secondary metabolites and ethyl acetate is the best extractant solvent. The results of this study confirmed the potential of Salvia hypoleuca for medicinal uses, especially for diabetes and skin diseases. Manuscript profile