The raspberry (Rubus ulmifolius subsp. Sanctus) is the perennial shrub in the genus Rubus of the Rosacea family. It is a medicinal and edible fruit throughout much of Khandaracy region of Urmia. Raspberry fruits are most important in food and medicinal sciences due to their excellent antioxidant activity and various flavonoids and proanthocyanin content. In this research, after harvesting fruits from the Khandaracy region of Urmia in the late summer, pH level by pH meter, organic acids by titration with 0.1 molar NaOH, Soluble solids using a refractometer, total phenolic content (by using Folin–Ciocalteu assays), total flavonoid content (aluminum chloride method), antioxidant capacity by two methods (DPPH and FRAP), total anthociyanin content (pH differential method), L-phenylalanine ammonia-lyase (PAL) and (guaiacol peroxidase) G-POD enzymes were determined. The results showed that level pH, organic acids and soluble solids were respectively 3.13, 1.14, 12.6, also values of total phenol content, total flavonoid, antioxidant activity and total anthocyanin content were 26.44 mg GAE.ml-1 Extract, 1.99 mg Qu. ml-1 extract, 55.29 % and 63.78 cyanidin-3-glucoside ml-1 Extract respectively. The results of this study showed that raspberry fruit has high content of polyphenol compounds and has significant antioxidant activity, so it can be used in food and pharmaceutical industries.
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