بررسی میزان آفلاتوکسین M1 در شیر خام مراکز جمع آوری شیر شهرهای چالوس و رامسر
محورهای موضوعی :
علوم و صنایع غذایی
احمدرضا برامی
1
,
محمدرضا پور علمی
2
,
مهرداد ایرانی
3
1 - 1- دانشگاه آزاد اسلامی، واحد چالوس، گروه علوم آزمایشگاهی، چالوس، ایران.
2 - دانشگاه آزاد اسلامی، واحد چالوس، گروه علوم دامی، چالوس، ایران
3 - دانشگاه آزاد اسلامی، واحد قائم‌شهر، گروه علوم دامی، قائم‌شهر، ایران.
تاریخ دریافت : 1390/09/23
تاریخ پذیرش : 1391/03/10
تاریخ انتشار : 1390/12/01
کلید واژه:
رامسر,
شیر خام,
الایزا,
آفلاتوکسین M1,
چالوس,
چکیده مقاله :
آفلاتوکسین M1 در اثر مصرف غذای آلوده به آفلاتوکسین B1 توسط گاو، در شیر ظاهر می شود. در این مطالعه آلودگی شیر خام مراکز جمع آوری شیر خام شهرهای چالوس و رامسر به آفلاتوکسین M1 بررسی شد. تعداد 200 نمونه شیر خام در طول دو فصل زمستان (دی و بهمن) و تابستان (تیر و مرداد) از مراکز جمع آوری شیر خام تهیه گردید و به روش الایزا مورد بررسی قرار گرفت. آفلاتوکسین M1 در 100 و 79/59 درصد از نمونه های اخذ شده فصل زمستان به ترتیب در رامسر و چالوس ردیابی شد. از این میان 45 درصد از نمونه های رامسر و30 درصد از نمونه های چالوس بیش از حد قابل قبول (50 نانوگرم در لیتر) استاندارد ملی و استاندارد اتحادیه اروپا به آفلاتوکسین M1 آلوده بودند. تفاوت بین میانگین مقدار آفلاتوکسین M1 در نمونه های فصول زمستان و تابستان معنی دار نبود (05/0P<) در حالی که بین شهرهای چالوس و رامسر نتایج بدست آمده در هر دو فصل تفاوت معنی دار داشت (05/0P<). همچنین در طول مطالعه آلوده ترین ماه تحقیق، ماه بهمن تعیین گردید.
چکیده انگلیسی:
Aflatoxin M1 (AFM1) appears in milk as a direct result of the ingestion of feed contaminated with aflatoxin B1 by cattle. This study was conducted to investigate the contamination rate of raw milk whit aflatoxin M1 in Chaloos and Ramsar raw milk collection centers. Two hundred bulk raw milk samples were collected during winter (January and February) and summer (June and July) seasons. The milk samples were analyzed by ELISA method for the presence of AFM1. During the winter, AFM1 was detected in 100% and 59/79% of the bulk raw milk samples in Ramsar and Chaloos, respectively; however, during summer 83/52% and 50/1 of the samples was found as positive in Ramsar and Chaloos, respectively. Furthermore, 45% of Ramsar and 30% of Chaloos bulk milk samples showed higher contamination level of AFM1 than maximum tolerance limit (50 ng/l) accepted by National Standard as well as European Union. Although, the difference between the contamination rate in samples obtained during summer and winter seasons was not statistically significantly, (p<0/05) significant difference (p<0/05) was observed between Chaloos and Ramsar samples in each season. Moreover, highest contamination rate was found in February.
منابع و مأخذ:
Alborzi, S., Pourabbas, B. and Rashidi, M. (2006). Aflatoxin M1 contamination in pasteurized milk in Shiraz. Food Control, 17: 582-594.
Bakirici, I. (2001). A study on the occurrence of Aflatoxin M1 in milk and milk products produced in Van of turkey. Food Control, 12(1): 47-51.
Buchi, G. and Weinreb, S.M. (1969). Total synthesis of racemic Aflatoxin M1 (milk toxin). Journal of the American Chemical Society, 91(19): 5408-5409.
Caravajal, M., Bolanos, A., Rojo, F. and Mendez, I. (2003). Aflatoxin M1 in pasteurized and ultrapasteurized milk with different fat content in Mexico. Journal of Food Protection, 66: 1885-92.
Caravajal, M., Rojo, F., Mendez, I. and Bolanos, A. (2003). Aflatoxin B1 and its interconverting metabolite aflatoxin in milk: the situation in Mexico. Food Additives and Contaminants, 20: 1077-1086.
Galvano, F., Galofaro, V., Ritieni, A., Bognanno, M., De angelis, A. and Galvano, G. (2001). Survey of the occurrernce of aflatoxin M1 in dairy products marketed in Italy: second year of observation. Food Additives and Contaminants, 18(7): 644-656.
Ghiasian, S.A., Maghsood, A.H., Neyestani, T. and Mirhendi, S.H. (2007). Occurrence of Aflatoxin M1 in raw milk during the summer and winter seasons in Hamedan, Iran. Journal of Food Safety, 27: 188-198.
Gholampour, A.I., Khoushnevis, S.H. and Hashemi, S.J. (2007). Aflatoxin M1 level in pasteurized and sterilized milk of Babol city. Tehran University Medical Journal, 65: 20-24 [In Farsi].
Hussein, H.S. and Brasel, J.M. (2001). Toxicity, metabolism, and impact of mycotoxins on humans and animals, Toxicology, 167: 101-134.
Ismair, Y. and Ruston, S. (1996). Aflatoxin in food and feed: occurrence, legislation and inactivation by physical mthods. Food Chemistry, 59(1): 57-67.
Kamkar, A. (2008). Detection of Aflatoxin M1 in powdered milk samples by ELISA. Pajouhesh-va-Sazandegi, 79: 174-180
Kamkar, A. (2005). A study on the occurrence of Aflatoxin M1 in raw milk produced in sarab city of iran. Food Control, 16(7): 593-599.
Karim, G., Bokaei, S. and Khorasani, A. (1982). Study on the contamination of milk with Aflatoxin in Tehran. Jouranl of Iran Public Health, 11: 19-23 [In Farsi].
Karim, G., Bokaei, S. and Khorasani, A. (1998). Contamination of raw milk with Aflatoxin M1 in Tehran using ELISA. pajoohesh Journal, 40(3): 163-5 [In Farsi].
Lafont, P., Siriwardana, M. and Lafont, J. (1989). Genotoxicity of hydroxyl-Aflatoxins M1 and M4. Microbiology Alimentarius Nutrition, 7: 1-8.
Letutour, B., Tantaovi, A. and Ihal, L. (1983). Simulaneous detection of alfaroxin B1 andochratoxin A in olive oil. Journal of the American Oil Chemistery Society, 60(4): 835-837.
Martins, M.L. and Martins, H.M. (2000). Aflatoxin M1 in raw and ultra high temperature-treated milk commercialized in Portugal. Food Additives and Contamination, 17: 871-874.
Nemati, M., MesgariAbbasi, M., Parsa, H. and Ansarin, M.A. (2010). Survey on the occurrence of Aflatoxin M1 in milk samples in Ardabil, iran. Food Control, 21(7): 1022-1024.
Oliveria, C.A., Germano, P.M., Bird, C. and Pinto, C.H. (1997). Immunochemical assessment of Aflatoxin M1 in milk powder consumed by infants in Sao Paulo, Brazil. Food additives and contaminants, 14(1): 7-10.
Oruc, H.H. and Sonal, S. (2001). Determination of Aflatoxin M1 levels in cheese and milk consumed in bursa, Turkey. Veterinary and human Toxicology, 43(5): 292-303.
Oveisi, M.R., Jannat, B., Sadeghi, N., Hajimahmoodi, M. and Nikzad, A. (2007). Presence of Aflatoxin M1 in milk and infant milk production Tehran, iran. Food Control, 18: 1216-1218.
Purchase, F.H. (1963). Acute toxicity of Aflatoxins M1 and M2 in one-day-old Ducklings. Food and Cosmetics Toxicology, 5: 339-342.
Razavilar, V. (2001). Pathogenic Microorganisms in Foods and Epidemiology of Food Poisoning. (2001). Tehran University Press, Tehran, Iran, pp. 189-240 [In Farsi].
Riazipour, M., Tavokoli, H.R., Razzaghi-Abyaneh, M., Rafati, H. and Sadr Momtaz, M.T. (2010). Measuring the amount of M1 Aflatoxin in pasteurized milk. Kowsar Medical Journal, 15: 89-93 [In Farsi].
Rostogi, S., Dwivedi, D.P. and Khnna, K.S. (2004). Detection of Aflatoxin M1 contamination in milk and infant milk products from Indian markets by ELISA. Food Control, 15: 287-290.
Sefidgar, S.A., Azizi, G., Khosravani, A.R. and roudbar, M.S. (2008). Presense of Aflatoxin M1 in raw milk at cattle farms in babol, iran. Pakistan Iournal of Biological Science, 99(3): 484-486.
Tajik, H., Rohani, S.M. and Moradi, M. (2007). Detection of Aflatoxin M1 in raw and commercial pasteurized milk in urmia, iran. Pakistan Iournal of Biological Science, 10(22): 4103-4107.
Tajkarimi, M., Ghaemmaghami, S.S., Motalebi, A., Poursoltani, H., Salahnejad, A. and Shojaee, F. (2007). Seasonal survey in content M1 Aflatoxin in raw milk taken from 15 dairy factory. Pajouhesh-va-Sazandegi, 75: 2-9 [In Farsi].
You-min, F.U. (1996). Determination of Aflatoxin M1 in milk and product using immuno-affinity columnand fluorescence measurements. Journal of Food and Drug Analysis, 4(2): 175-183.
