بررسی میزان آفلاتوکسین M1 در شیر خام مراکز جمع آوری شیر شهر‌های چالوس و رامسر

محورهای موضوعی : علوم و صنایع غذایی

احمدرضا برامی ¹ (1- دانشگاه آزاد اسلامی، واحد چالوس، گروه علوم آزمایشگاهی، چالوس، ایران.)
محمدرضا پور علمی ² (دانشگاه آزاد اسلامی، واحد چالوس، گروه علوم دامی، چالوس، ایران)
مهرداد ایرانی ³ (دانشگاه آزاد اسلامی، واحد قائم‌شهر، گروه علوم دامی، قائم‌شهر، ایران.)

تاریخ دریافت : 1390/09/23 تاریخ پذیرش : 1391/03/10 تاریخ انتشار : 1390/12/01

کلید واژه: رامسر, شیر خام, الایزا, آفلاتوکسین M1, چالوس,

چکیده مقاله :

آفلاتوکسین M1 در اثر مصرف غذای آلوده به آفلاتوکسین B1 توسط گاو، در شیر ظاهر می شود. در این مطالعه آلودگی شیر خام مراکز جمع آوری شیر خام شهرهای چالوس و رامسر به آفلاتوکسین M1 بررسی شد. تعداد 200 نمونه شیر خام در طول دو فصل زمستان (دی و بهمن) و تابستان (تیر و مرداد) از مراکز جمع آوری شیر خام تهیه گردید و به روش الایزا مورد بررسی قرار گرفت. آفلاتوکسین M1 در 100 و 79/59 درصد از نمونه های اخذ شده فصل زمستان به ترتیب در رامسر و چالوس ردیابی شد. از این میان 45 درصد از نمونه های رامسر و30 درصد از نمونه های چالوس بیش از حد قابل قبول (50 نانوگرم در لیتر) استاندارد ملی و استاندارد اتحادیه اروپا به آفلاتوکسین M1 آلوده بودند. تفاوت بین میانگین مقدار آفلاتوکسین M1 در نمونه های فصول زمستان و تابستان معنی دار نبود (05/0P<) در حالی که بین شهرهای چالوس و رامسر نتایج بدست آمده در هر دو فصل تفاوت معنی دار داشت (05/0P<). همچنین در طول مطالعه آلوده ترین ماه تحقیق، ماه بهمن تعیین گردید.

چکیده انگلیسی:

Aflatoxin M1 (AFM1) appears in milk as a direct result of the ingestion of feed contaminated with aflatoxin B1 by cattle. This study was conducted to investigate the contamination rate of raw milk whit aflatoxin M1 in Chaloos and Ramsar raw milk collection centers. Two hundred bulk raw milk samples were collected during winter (January and February) and summer (June and July) seasons. The milk samples were analyzed by ELISA method for the presence of AFM1. During the winter, AFM1 was detected in 100% and 59/79% of the bulk raw milk samples in Ramsar and Chaloos, respectively; however, during summer 83/52% and 50/1 of the samples was found as positive in Ramsar and Chaloos, respectively. Furthermore, 45% of Ramsar and 30% of Chaloos bulk milk samples showed higher contamination level of AFM1 than maximum tolerance limit (50 ng/l) accepted by National Standard as well as European Union. Although, the difference between the contamination rate in samples obtained during summer and winter seasons was not statistically significantly, (p<0/05) significant difference (p<0/05) was observed between Chaloos and Ramsar samples in each season. Moreover, highest contamination rate was found in February.

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