ارایه روشی حساس و گزینش‌پذیر برای اندازه‌گیری تترا بوتیل بنزوکینون در روغن‌های خوراکی
محورهای موضوعی :
علوم و صنایع غذایی
هستی پورمددکار
1
(کارشناسی ارشد مهندسی پلیمر، دانشکده مهندسی نساجی و پلیمر، دانشگاه آزاد اسلامی واحد یزد، یزد، ایران)
نوید نصیری زاده
2
(دانشیار گروه پلیمر، دانشکده مهندسی نساجی و پلیمر، دانشگاه آزاد اسلامی واحد یزد، یزد، ایران)
سعید جعفری
3
(دانش‌آموخته کارشناسی‌ارشد مهندسی پلیمر، دانشکده مهندسی نساجی و پلیمر، دانشگاه آزاد اسلامی واحد یزد، یزد، ایران)
محمد دهقانی
4
(دانشجوی دکترا نساجی، باشگاه پژوهشگران جوان و نخبگان، دانشگاه آزاد اسلامی واحد یزد، یزد، ایران)
تاریخ دریافت : 1397/06/17
تاریخ پذیرش : 1398/03/01
تاریخ انتشار : 1398/04/01
کلید واژه:
روغن خوراکی,
پلیمر قالب مولکولی,
ترشری بوتیل بنزوکینون,
الکترود کربن سرامیکی,
چکیده مقاله :
حضور مقادیر کم ترشری بوتیل هیدروکینون یا محصول متابولیتی آن، تترا بوتیل بنزوکینون (TBQ)، به‌دلیل تمایل زیاد به گروه‌های تیول‌دار پروتئین‌ها یا دیواره سلول‌ها ممکن است مانع تکثیر سلولی و بروز ناهنجاری‌های بیولوژیک شوند. هدف از این پژوهش ساخت یک نانوحسگر الکتروشیمیایی برپایه پلیمر قالب مولکولی برای شناسایی TBQ در نمونه‌های روغن خوراکی می‌باشد. این مطالعه از نوع متدولوژیک بوده و جامعه آماری شامل نمونه‌های روغن خوارکی حاوی TBQ است. تأثیر عوامل مختلف نظیر مقدار پلیمر قالب مولکولی و نانولوله‌های کربنی در ساخت الکترود کربن سرامیکی اصلاح شده و هم‌چنین pH محلول پیش تغلیظ و زمان اقامت نانوحسگر تهیه شده در محلول پیش تغلیظ بر میزان جریان حاصل از اکسایش TBQ براساس روش سطح پاسخ بهینه‌سازی شد. برای تعیین مقدار TBQ موجود در نمونه‌های روغن از روش ولتامتری پالس تفاضلی استفاده شده است. مورفولوژی پلیمرهای قالب مولکولی و حسگر تهیه شده با میکروسکوپ الکترونی روبشی تشریح شد. شرایط بهینه برای جداسازی و اندازه‌گیری TBQ در روغن خوراکی شامل 10 میلی‌گرم نانولوله کربنی چند دیواره، 30 میلی‌گرم پلیمر قالب مولکولی برای تهیه الکترود کربن سرامیکی اصلاح شده به‌عنوان نانوحسگر و مدت زمان 8 دقیقه در محلول حاوی بافر فسفات 1/0 مولار با 0/10=pH حاصل گردید. روش پیشنهادی قادر به شناسایی TBQ در نمونه‌های روغن در محدوده غلظتی 680-6 نانومولار با حد تشخیص 1/3 نانومولار می‌باشد. بر اساس نتایج به‌دست آمده، روش پیشنهادی مبتنی بر حسگر تهیه شده می‌تواند به‌عنوان ابزاری مناسب جهت اندازه‌گیری TBQ در نمونه‌های روغن خوراکی در صنایع و آزمایشگاه‌های تخصصی به‌کار برده شود.
چکیده انگلیسی:
The presence of trace amount of Tetra -butyl hydroquinone or its metabolite, Tetra -butyl benzoquinone (TBQ), may inhibit cell proliferation and cause biologic abnormalities due to the high prevalence of thiolate groups of proteins or cell walls. The aim of this study was to fabrication an electrochemical nanosensor based on molecular imprinted polymer to detection of TBQ in edible oil. This study was a methodologic study. The statistical population included edible oil samples containing TBQ. The effect of different factors such as amount of MIP and MWCNT for production of ceramic carbon electrodes as well as pH of preconcentration solution and the incubation time of the prepared nanosensor in the solution on the oxidation signal of TBQ was optimized by response surface methodology. Differential pulsed voltammetry has been used to determine the TBQ in oil samples. The Morphologies of MIP and prepared sensors were described by scanning electron microscopy. Optimal conditions for the separation and determination of TBQ in edible oil, including 10 mg of multiwall carbon nanotube, 30 mg of MIP for preparing a modified carbon ceramic electrode, and 8 minutes as incubation time in a 0.1 M phosphate buffer solution with pH 10 was obtained. The proposed method is capable of detecting TBQ in edible oil samples at a concentration range of 6 - 680 nM with a detection limit of 3.1 nM. Based on the results, the proposed sensor can be used as a suitable tool for determination of TBQ in edible oil samples in industries and laboratories.
منابع و مأخذ:
· F., Seifati, S.M. and Nasirizadeh, N. (2017). Development of a DNA biosensor for detection of phenylketonuria based on screen-printed gold electrode and hematoxylin. Analytical Methods, 9(6): 966-973.
· Aghili, Z., Nasirizadeh, N., Divsalar, A., Shoeibi, S. and Yaghmaei, P. (2017). A nanobiosensor composed of exfoliated graphene oxide and gold nano-urchins, for detection of GMO products. Biosensors and Bioelectronics, 95: 72-80.
· Aluyor, E., Oboh, I. and Okieimen, C. (2009). The effect of tertiary butyl hydroquinone on the biodegradability of palm olein. Leonardo Electronic Journal of Practices and Technologies, 14: 47-56.
· Azadmard-Damirchi, S. and Torbati, M. (2015). Adulterations in Some edible oils and fats and their detection methods. Journal of Food Quality Hazard Content, 2: 38-44.
· Dechtrirat, D., Sookcharoenpinyob, B., Prajongtata, P., Sriprachuabwongac, C., Sanguankiatd, A. Tuantranontc, A. et al., (2018). An electrochemical MIP sensor for selective detection of salbutamol based on a graphene/PEDOT: PSS modified screen printed carbon electrode, RSC Advanced, 8: 206-212.
· Ding, M. and Zou, J. (2012). Rapid micropreparation procedure for the gas chromatographic-mass spectrometric determination of BHT, BHA and TBHQ in edible oils. Food Chemistry, 131: 1051-1055.
· Dorni, C., Sharma, P., Saikia, G. and Longvah, T. (2018). Fatty acid profile of edible oils and fats consumed in India. Food Chemistry, 238: 9-15.
· Elshafie, M.M., Nawar, I.A., Algamal, M.A. and Ahmad, S.M. (2012). Evaluation of the biological effects for adding cinnamon volatile oil and tbhq as antioxidant on rats’ lipid profiles. Asian Journal of Plant Science, 11: 100-108.
· Esfandiyari, T., Nasirizadeh, N., Dehghani, M. and Ehrampoosh, M.H. (2017). Graphene oxide based carbon composite as adsorbent for Hg removal: preparation, characterization, kinetics and isotherms studies. Chinese Journal of Chemical Engineering, 25: 1170–1175.
· Espinosa-Mansilla, A., Salinas, F., Olmo, M. and Payá, I.O. (1996). Determination of synthetic food antioxidants mixtures using UV-visible spectrophotometry and partial least-squares calibration. Applied Spectroscopy, 50: 449-453.
· Etemadifar, A., Dehghanizadeh, H., Nasirizadeh, N. and Rohani-Moghadam, M. (2014). Statistical optimization of wool dyeing with alizarin red s as a natural dye via central composite design. Fibers and Polymers, 15 (2): 254-260.
· Goulart, L.A., Teixeira, A.R.L., Ramalho, D.A., Terezo, A.J. and Castilho, M. (2014). Development of an analytical method for the determination of tert-butylhydroquinone in soybean biodiesel. Fuel, 115:126–131.
· Hajisafari, M. and Nasirizadeh N. (2017). An electrochemical nanosensor for simultaneous determination of hydroxylamine and nitrite using oxadiazole self–assembled on silver nanoparticles modified glassy carbon electrode. Ionics, 23 (6): 1541-1551.
· Jafari, S., Dehghani, M. and Nasirizadeh, N. (2017). Developing a highly sensitive electrochemical sensor using thiourea-imprinted polymers based on an MWCNT modified carbon ceramic electrode. Journal of Electroanalytical Chemistry, 802: 139–146.
· Jafari, S., Dehghani, M., Nasirizadeh, N, and Azimzadeh, M. (2018). An azithromycin electrochemical sensor based on an aniline MIP film electropolymerized on a gold nano urchins/graphene oxide modified glassy carbon electrode, Journal of Electroanalytical Chemistry, 829: 27-34.
· Jafari, S., Dehghani, M., Nasirizadeh, N. and Akrami, H. (2018). Voltammetric determination of basic red 13 during its sonoelectrocatalysis degradation. Microchimica Acta, 184 (11): 4459–4468.
· Khadem, M., Faridbod, F., Norouzi, P., Rahimi Foroushani, A.F., Ganjali, M.R., Shahtaheri, S.J. et al., (2016). Development of a specific electrochemical sensor for occupational and environmental monitoring of diazinon. Journal of Health and Safety at Work, 7 (1): 9-22. [In Persian]
· Khadem, M., Faridbod, F., Norouzi, P., Rahimi Foroushani, A.F., Ganjali, M.R., Shahtaheri, S.J. et al., (2017). Designing and development of an electrochemical sensor modified with molecularly imprinted polymer and carbon nanotubes for evaluation of occupational and environmental exposures to dicloran pesticide .Iran Occupational Health, 14(3): 1-11.[In Persian]
· Li, J., Bi, Y., Liu, W. and Sun, S. (2015). Simultaneous analysis of tertiary butylhydroquinone and 2-tert-butyl-1, 4-benzoquinone in edible oils by normal-phase high-performance liquid chromatography. Journal of Agriculture and Food Chemistry, 63(38):8584-8591.
· Motaharian, A. and Milani-Hosseini, M.R. (2016). Electrochemical sensor based on molecularly imprinted polymer nanoparticles for determination of diazepam drug. Journal of Applied Research in Chemistry, 9(3):51-59.
· Nasirizadeh, N., Shekari, Z., Nazari, A. and Tabatabaee, M. (2016). Fabrication of a novel electrochemical sensor for determination of hydrogen peroxide in different fruit juice samples. Journal of Food and Drug Analysis, 24: 72-82.
· Pan, Y., Lai, K., Fan, Y., Li, C., Pei, L., Rasco, B.A. et al., (2014). Determination of tert-butylhydroquinone in vegetable oils using surface-enhanced Raman spectroscopy. Journal of Food Science, 79(6): 1225-1230.
· Selvolini, G. and Marrazza, G. (2017). MIP-based sensors: promising new tools for cancer biomarker determination. Sensors (Basel), 17 (4): 718-730.
· Shojaei, S., Nasirizadeh, N., Entezam, M., Koosha, M. and Azimzadeh, M. (2016). An electrochemical nanosensor based on molecularly imprinted polymer (mip) for detection of gallic acid in fruit juices. Food Analytical Methods, 9: 2721–2731.
· Silva, M.M. and Lidon, F.C. (2016). An overview on applications and side effects of antioxidant food additives. Emirates Journal of Food and Agriculture, 28(12): 823-832.
· Thomas, A., Vikraman, A.E., Thomas, D. and Kumar, K.G. (2015). Voltammetric sensor for the determination of TBHQ in coconut oil. Food Analytical Methods, 8: 2028-2034.
· Tormin, T.F., Cunha, R.R., Richter, E.M. and Munoz, R.A.A. (2012). Fast simultaneous determination of BHA and TBHQ antioxidants in biodiesel by batch injection analysis using pulsed-amperometric detection. Talanta, 99: 527-531.
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· F., Seifati, S.M. and Nasirizadeh, N. (2017). Development of a DNA biosensor for detection of phenylketonuria based on screen-printed gold electrode and hematoxylin. Analytical Methods, 9(6): 966-973.
· Aghili, Z., Nasirizadeh, N., Divsalar, A., Shoeibi, S. and Yaghmaei, P. (2017). A nanobiosensor composed of exfoliated graphene oxide and gold nano-urchins, for detection of GMO products. Biosensors and Bioelectronics, 95: 72-80.
· Aluyor, E., Oboh, I. and Okieimen, C. (2009). The effect of tertiary butyl hydroquinone on the biodegradability of palm olein. Leonardo Electronic Journal of Practices and Technologies, 14: 47-56.
· Azadmard-Damirchi, S. and Torbati, M. (2015). Adulterations in Some edible oils and fats and their detection methods. Journal of Food Quality Hazard Content, 2: 38-44.
· Dechtrirat, D., Sookcharoenpinyob, B., Prajongtata, P., Sriprachuabwongac, C., Sanguankiatd, A. Tuantranontc, A. et al., (2018). An electrochemical MIP sensor for selective detection of salbutamol based on a graphene/PEDOT: PSS modified screen printed carbon electrode, RSC Advanced, 8: 206-212.
· Ding, M. and Zou, J. (2012). Rapid micropreparation procedure for the gas chromatographic-mass spectrometric determination of BHT, BHA and TBHQ in edible oils. Food Chemistry, 131: 1051-1055.
· Dorni, C., Sharma, P., Saikia, G. and Longvah, T. (2018). Fatty acid profile of edible oils and fats consumed in India. Food Chemistry, 238: 9-15.
· Elshafie, M.M., Nawar, I.A., Algamal, M.A. and Ahmad, S.M. (2012). Evaluation of the biological effects for adding cinnamon volatile oil and tbhq as antioxidant on rats’ lipid profiles. Asian Journal of Plant Science, 11: 100-108.
· Esfandiyari, T., Nasirizadeh, N., Dehghani, M. and Ehrampoosh, M.H. (2017). Graphene oxide based carbon composite as adsorbent for Hg removal: preparation, characterization, kinetics and isotherms studies. Chinese Journal of Chemical Engineering, 25: 1170–1175.
· Espinosa-Mansilla, A., Salinas, F., Olmo, M. and Payá, I.O. (1996). Determination of synthetic food antioxidants mixtures using UV-visible spectrophotometry and partial least-squares calibration. Applied Spectroscopy, 50: 449-453.
· Etemadifar, A., Dehghanizadeh, H., Nasirizadeh, N. and Rohani-Moghadam, M. (2014). Statistical optimization of wool dyeing with alizarin red s as a natural dye via central composite design. Fibers and Polymers, 15 (2): 254-260.
· Goulart, L.A., Teixeira, A.R.L., Ramalho, D.A., Terezo, A.J. and Castilho, M. (2014). Development of an analytical method for the determination of tert-butylhydroquinone in soybean biodiesel. Fuel, 115:126–131.
· Hajisafari, M. and Nasirizadeh N. (2017). An electrochemical nanosensor for simultaneous determination of hydroxylamine and nitrite using oxadiazole self–assembled on silver nanoparticles modified glassy carbon electrode. Ionics, 23 (6): 1541-1551.
· Jafari, S., Dehghani, M. and Nasirizadeh, N. (2017). Developing a highly sensitive electrochemical sensor using thiourea-imprinted polymers based on an MWCNT modified carbon ceramic electrode. Journal of Electroanalytical Chemistry, 802: 139–146.
· Jafari, S., Dehghani, M., Nasirizadeh, N, and Azimzadeh, M. (2018). An azithromycin electrochemical sensor based on an aniline MIP film electropolymerized on a gold nano urchins/graphene oxide modified glassy carbon electrode, Journal of Electroanalytical Chemistry, 829: 27-34.
· Jafari, S., Dehghani, M., Nasirizadeh, N. and Akrami, H. (2018). Voltammetric determination of basic red 13 during its sonoelectrocatalysis degradation. Microchimica Acta, 184 (11): 4459–4468.
· Khadem, M., Faridbod, F., Norouzi, P., Rahimi Foroushani, A.F., Ganjali, M.R., Shahtaheri, S.J. et al., (2016). Development of a specific electrochemical sensor for occupational and environmental monitoring of diazinon. Journal of Health and Safety at Work, 7 (1): 9-22. [In Persian]
· Khadem, M., Faridbod, F., Norouzi, P., Rahimi Foroushani, A.F., Ganjali, M.R., Shahtaheri, S.J. et al., (2017). Designing and development of an electrochemical sensor modified with molecularly imprinted polymer and carbon nanotubes for evaluation of occupational and environmental exposures to dicloran pesticide .Iran Occupational Health, 14(3): 1-11.[In Persian]
· Li, J., Bi, Y., Liu, W. and Sun, S. (2015). Simultaneous analysis of tertiary butylhydroquinone and 2-tert-butyl-1, 4-benzoquinone in edible oils by normal-phase high-performance liquid chromatography. Journal of Agriculture and Food Chemistry, 63(38):8584-8591.
· Motaharian, A. and Milani-Hosseini, M.R. (2016). Electrochemical sensor based on molecularly imprinted polymer nanoparticles for determination of diazepam drug. Journal of Applied Research in Chemistry, 9(3):51-59.
· Nasirizadeh, N., Shekari, Z., Nazari, A. and Tabatabaee, M. (2016). Fabrication of a novel electrochemical sensor for determination of hydrogen peroxide in different fruit juice samples. Journal of Food and Drug Analysis, 24: 72-82.
· Pan, Y., Lai, K., Fan, Y., Li, C., Pei, L., Rasco, B.A. et al., (2014). Determination of tert-butylhydroquinone in vegetable oils using surface-enhanced Raman spectroscopy. Journal of Food Science, 79(6): 1225-1230.
· Selvolini, G. and Marrazza, G. (2017). MIP-based sensors: promising new tools for cancer biomarker determination. Sensors (Basel), 17 (4): 718-730.
· Shojaei, S., Nasirizadeh, N., Entezam, M., Koosha, M. and Azimzadeh, M. (2016). An electrochemical nanosensor based on molecularly imprinted polymer (mip) for detection of gallic acid in fruit juices. Food Analytical Methods, 9: 2721–2731.
· Silva, M.M. and Lidon, F.C. (2016). An overview on applications and side effects of antioxidant food additives. Emirates Journal of Food and Agriculture, 28(12): 823-832.
· Thomas, A., Vikraman, A.E., Thomas, D. and Kumar, K.G. (2015). Voltammetric sensor for the determination of TBHQ in coconut oil. Food Analytical Methods, 8: 2028-2034.
· Tormin, T.F., Cunha, R.R., Richter, E.M. and Munoz, R.A.A. (2012). Fast simultaneous determination of BHA and TBHQ antioxidants in biodiesel by batch injection analysis using pulsed-amperometric detection. Talanta, 99: 527-531.
