List of Articles shoot regeneration

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  1 - Study of Direct Adventitious Shoot Regeneration in Pomegranate ( Punicagranatum cv. MalasSaveh ) through Cotyledonary Explants
  F. Soukhak A. Khalighi S. A. Ghaemmaghami
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  2 - Attempt in order to Micropropagation of Linden In vitro
  Kamal Ghasemi Bezdi Vahideh Payamnour Mostafa Mehrdad Akram Ahmadi
  10.22034/jest.2017.10345
  Background and: Linden is one of threatened species in north of Iran that form very small percentage of the northern forests. Since the linden seeds are facing with germination problems such as with dual physiological (the embryo) and mechanical (hard cover pericarp) do More

  Background and: Linden is one of threatened species in north of Iran that form very small percentage of the northern forests. Since the linden seeds are facing with germination problems such as with dual physiological (the embryo) and mechanical (hard cover pericarp) dormancy that application such as tissue culture can be effective in planting, restoration and conservation of genetic resources. Method: This study was carried out in order to determine the most suitable explants and the best hormone combination to optimize micropropagation of linden in vitro. Explants were collected and cultured randomly from the best individual of linden from Gorgan Touskaestan forest. Findings: In light of results, from the eleven tested explants, stem containing terminal buds with scale were selected as the most suitable explants and IBA 0.1 mg/l was selected as the most appropriate medium hormonal combination in shoot regeneration. Discussion and Conclusion: Obtained data were analyzed with a completely randomized design by MSTATC software. It is hoped that the achieved results can be reproduced and be helpful in tissue culture research and also the molecular basis studies Manuscript profile
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  3 - Effects of Multi-Wall Carbon Nano Tube (MWCNT) on Nepeta cataria and Salvia sclarea In Vitro culture
  Hekmat A likhani Mehrjardi Parissa Jonoubi Ahmad Majd Reza Haji Hosseini
  10.30495/ijpp.2022.1953545.1406
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  4 - Promotion of callus initiation, shoot regeneration and proliferation in Lisianthus.
  Seied Mehdi Miri Akram Savari Kamelia Behzad Behrad Mohajer Iravani
  10.30495/ijpp.2016.532656
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  5 - Indirect regeneration of endangered Dracocephalum kotschyi Boiss
  parisa jonubi Ahmad Majd mitra zamani nasrabadi
  Daracocephalum Kotschyi Boiss is a pharmaceutical and aromatic species of Lamiaceae which could be found in the northern and the north-eastern highlands of Iran. D. Kotschyi is an endangered species due to specific circumstances of habitat and human indiscriminated harv More

  Daracocephalum Kotschyi Boiss is a pharmaceutical and aromatic species of Lamiaceae which could be found in the northern and the north-eastern highlands of Iran. D. Kotschyi is an endangered species due to specific circumstances of habitat and human indiscriminated harvest. In vitro culture of different explants from sterile seedlings showed that all of media used for hypocotyl just produced callus without any shoot regeneration. The best callus production obtained from 14 day old - cotyledonary leaves cultured on MS medium containing 0.5 mg l-1 BA and 0.5 mg l-1 NAA. These calli were subcultured to MS medium containing 2 mg l-1 BA and 0.2 mg l-1 NAA that showed 33.3% shoot regeneration. In order to indirect regeneration using sequential reculturing, cotyledonary leaves on MS medium containing 1 mg l-1 BA and 1 mg l-1 NAA that 53.3% shoot regeneration was obtained. 75% from regenerated plants that were subcultured on root induction medium supplemented by 2 mg l-1 NAA could produce the strong roots. These plants were transferred to vermiculite and soil.They were adapted to greenhouse condition. Manuscript profile
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  6 - Plant regeneration from blackberry lateral bud culture under a set of hormone, silicic acid, sucrose and activated charcoal
  Abbas Ali Dehpour joybari saeid soltani Roya Bishehkolaei kamran ghasemi zahra rajabzadeh
  10.30495/jdb.2021.686504
  The objective of this work was to evaluate the effect plant growth regulator, silicic acid, sucrose and activated charcoal used in micro propagation of thornless Blackberry cv. Merton explants. Propagules of cv. Merton were cultivated on a 1/2 Murashige and Skoog (MS) m More

  The objective of this work was to evaluate the effect plant growth regulator, silicic acid, sucrose and activated charcoal used in micro propagation of thornless Blackberry cv. Merton explants. Propagules of cv. Merton were cultivated on a 1/2 Murashige and Skoog (MS) medium. The single buds were cultured in 1/2 MS containing 0.1% activated charcoal and new shoot containing buds were formed 3 weeks after culture. New buds were cultured in MS containing BA, Kin and IBA at concentrations of 0.2, 0.5, 1, 1.5 and 2 mgL-1 for shoot regeneration. New shoot after 6 weeks regenerated and the best medium for shoot regeneration was MS medium contains 1.5 mg/l BA and 0.1 mg/l IBA. Shoots were cultured in 1/2 MS containing 0.2, 0.5, 1, 2 mgL-1 NAA and IBA for root formation. After determining the optimal concentrations of IBA and NAA, activated charcoal in concentrations of 0.01, 0.02, 0.05, 0.1, 0.2%, silicic acid in concentrations of 0, 1, 5, 10, 20 mgL-1, sucrose in concentrations of 1, 2, 3, 4, 5% were added to the culture medium to improve rooting. Sucrose in 4% increased the root length, root weight and chlorophyll content. The addition of activated charcoal in the MS medium resulted in increased shoot and root length. In thornless blackberry cultures, Silicic acid decreased the shoot number but until 5 mg/l increased the shoot length. Silicic acid increased the root length in 1 mg/l and increased the root weight in 5 mg/l rather than control respectively. Manuscript profile
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  7 - The studies of different culture methods in ors (Juniperus seravshanica)
  Farkhondeh Rezanejad Farzaneh Farzan Elaheh ZamaniBahramabadi Farzad Ganjalikhani hakemi
  10.30495/jdb.2023.1967001.1332
  Juniperus is the second most prevalent group of conifers on Earth. The reduction in viable seeds and natural regeneration is an important problem of Juniperus. Juniperus seravschanica is important in soil protection and is very resistant to frost and drought too. In thi More

  Juniperus is the second most prevalent group of conifers on Earth. The reduction in viable seeds and natural regeneration is an important problem of Juniperus. Juniperus seravschanica is important in soil protection and is very resistant to frost and drought too. In this study, seed number in cone, seed emptiness and germination were studied on filter paper, peatmoss+ perlite mixture and in field in Galuchar, sarbijan and Dalfard (Kerman province) in populations. In addition, shoot proliferation and rooting in pot and in vitro culture was assayed. The average number of cone seeds in Glochar, Serbijan and Delfard was 4.86, 3.69 and 3.21, respectively. The average number of full seeds per cone was 1.1, 0.22 and 0.1 (22, 5.96 and 3.1%, respectively) in Glochar, Sarbijan and Dalfard, showing 78, 94 and 97% emptiness, respectively. In all media, seed germination percentage was very low (2-5%). No germination was observed in seeds without cold treatment as well as without scarification (making a hole in seed coat). 20 cm young shoots, after cold treatment and rapid immersion in IBA, grew as normal until 4 week and finally dried after 5-6 weeks. The young shoots (1-1.5 cm) cultured in MS and WPM media proliferated and regenerated new branches in WPM (40% regeneration) and MS (57%) media in combination of BAP and NAA (2+3 and 0.2+3 mgl-1, respectively for WPM and MS). No rooting was observed using IBA after 24 weeks. Manuscript profile
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  8 - High performant eggplant in vitro regeneration and organogenesis
  Farhad Taghipour Soleiman Jamshidi Fahrul Huyop
  10.22034/aej.2015.515858
  Eggplant is a notable vegetable crop grown in a variety of tropical and temperate regions. In vitro regeneration of eggplant was established to determine an efficient phytohormone concentration for development of organogenesis from cotyledon and leaf explants. Ten-day o More

  Eggplant is a notable vegetable crop grown in a variety of tropical and temperate regions. In vitro regeneration of eggplant was established to determine an efficient phytohormone concentration for development of organogenesis from cotyledon and leaf explants. Ten-day old cotyledon and 25-day old leaf explants were cultured on MS medium amended with Gamborg’s B5 vitamins and 2% sucrose in 10 different concentrations and combinations of NAA, BAP, TDZ, 2,4-D and IAA phytohormons. Cultured explants were incubated at 25 ± 2 ºC for 3 weeks under 16:8 h photoperiod. Well-grown regenerated shoots were transferred to fresh growth medium after 3 weeks for shoot elongation. All cotyledon and leaf explants produced various callus masses but only white and friable ones were able to regenerate into vigorous shoots. Shoot regeneration medium (SRM10) containing MS + TDZ 2 mg/L + BAP 0.5 mg/L + NAA 0.5 mg/L showed high potential in organogenesis of both explant types. Shoot regeneration and organogenesis from cotyledon explants were much more advantageous than leaf explants. The average of cotyledon and leaf explants producing shoot were 4.3 ± 0.33 and 1.6 ± 0.67 out of 5 in each plate, respectively. The percentages of regenerated shoots in cotyledon and leaf explants cultured on SRM10 were 86.6 ± 6.67 and 33.3 ± 13.3%, respectively. Current work can shed a light on production of transgenic eggplants and can be followed by normal mature plants regeneration. Manuscript profile
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  9 - The effect of halo- and hydropriming on germination and initial growth of corn cv. SC704 under salinity and drought stress conditions
  Alyeh Rokhfirooz Soleiman Jamshidi Naser Mohebbalipour
  10.22034/aej.2015.515941
  This study was conducted during thesummer of 2013 in Islamic Azad University, Dezful branch in a completely randomized design with three replications in a factorial base in order to determine the effect of hydro and halopriming pretreatments on germination, early growth More

  This study was conducted during thesummer of 2013 in Islamic Azad University, Dezful branch in a completely randomized design with three replications in a factorial base in order to determine the effect of hydro and halopriming pretreatments on germination, early growth and establishment of maize 704 single cross cultivar under salinity and drought conditions. To this end, the corn seeds pretreated with potassium nitrate (halopriming) and distilled water (hydropriming) in terms of germination index and growth of seedlings under salt stress by sodium chloride and drought conditions caused by poly ethylene glycol 6000 in water potentials of 0, -0.2, -0.4 and -0.6 MPa in greenhouse were studied in vitro. Seeds were able to germinate in all concentrations of sodium chloride solution, but germination was not observed only in -0.6 MPa osmotic potential. Pretreatment of seeds caused better seed germination and seedling growth under salinity and drought stresses. The application of hydro and osmotic pretreatments could compensate the negative effects of salinity and drought stresses and had positive effect on germination indices and also caused rapid seed germination. Generally, pretreatment practices are recommended for the improvement of germination and initial establishment of maize seedling. �مام غلظت­های محلول کلرید­سدیم بودند، اما تنها در پتانسیل اسمزی 6/0- مگاپاسکال جوانه­زنی مشاهده نشد. پیش­تیمار بذر باعث جوانه­زنی بهتر و رشد گیاهچه­ها تحت تنش­های شوری و خشکی ­شد. استفاده از روش پیش­تیمار اسمزی و آبی توانست اثرات منفی تنش­ها را جبران نماید و اثر مثبت بر شاخص­های جوانه­زنی داشت و باعث شد تا جوانه­زنی بذرها سریعتر انجام شود. در کل، اعمال پیش­تیمار برای بهبود جوانه­زنی و استقرار اولیه گیاهچه­ ذرت تحت تنش توصیه می­شود. Manuscript profile