High performant eggplant in vitro regeneration and organogenesis
Subject Areas : Agroecology Journal
Farhad Taghipour
1
,
Soleiman Jamshidi
2
,
Fahrul Huyop
3
1 - PhD in Bioscience
Biotechnology Department
Faculty of Biosciences & Medical Engineering
University Technology Malaysia
Johor, Malaysia
2 - باشگاه پژوهشگران جوان و نخبگان واحد میانه
دانشگاه آزاد اسلامی
میانه، ایران
3 - Professor of Biochemistry
Biotechnology Department
Faculty of Biosciences & Medical Engineering
University Technology Malaysia
Johor, Malaysia
Abstract :
Eggplant is a notable vegetable crop grown in a variety of tropical and temperate regions. In vitro regeneration of eggplant was established to determine an efficient phytohormone concentration for development of organogenesis from cotyledon and leaf explants. Ten-day old cotyledon and 25-day old leaf explants were cultured on MS medium amended with Gamborg’s B5 vitamins and 2% sucrose in 10 different concentrations and combinations of NAA, BAP, TDZ, 2,4-D and IAA phytohormons. Cultured explants were incubated at 25 ± 2 ºC for 3 weeks under 16:8 h photoperiod. Well-grown regenerated shoots were transferred to fresh growth medium after 3 weeks for shoot elongation. All cotyledon and leaf explants produced various callus masses but only white and friable ones were able to regenerate into vigorous shoots. Shoot regeneration medium (SRM10) containing MS + TDZ 2 mg/L + BAP 0.5 mg/L + NAA 0.5 mg/L showed high potential in organogenesis of both explant types. Shoot regeneration and organogenesis from cotyledon explants were much more advantageous than leaf explants. The average of cotyledon and leaf explants producing shoot were 4.3 ± 0.33 and 1.6 ± 0.67 out of 5 in each plate, respectively. The percentages of regenerated shoots in cotyledon and leaf explants cultured on SRM10 were 86.6 ± 6.67 and 33.3 ± 13.3%, respectively. Current work can shed a light on production of transgenic eggplants and can be followed by normal mature plants regeneration.
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