Attempt in order to Micropropagation of Linden In vitro

Subject Areas : environmental management

Kamal Ghasemi Bezdi ¹ , Vahideh Payamnour ² , Mostafa Mehrdad ³ , Akram Ahmadi ⁴

1 - Associate Professor, Department of Agricultural Research, Education and Extension Organization (AREEO), Cotton Research Institute of Iran, Gorgan, Iran. *(Corresponding Author).
2 - Associated professor, Faculty of forest sciences, Gorgan University of Agricultural Sciences & Natural Resources, Gorgan, Iran.
3 - M.Sc., Faculty of Forest Sciences, Gorgan University of Agricultural Sciences & Natural Resources Gorgan, Iran.
4 - Ph.D, Department of Forestry, Faculty of Agriculture and Natural Resource, Gonbad Kavous University, Gonbad, Iran

Received: 2015-05-06 Accepted : 2015-11-09 Published : 2017-03-21

Keywords: Linden, Medium, Explant, Shoot regeneration, Hormone,

Abstract :

Background and: Linden is one of threatened species in north of Iran that form very small percentage of the northern forests. Since the linden seeds are facing with germination problems such as with dual physiological (the embryo) and mechanical (hard cover pericarp) dormancy that application such as tissue culture can be effective in planting, restoration and conservation of genetic resources. Method: This study was carried out in order to determine the most suitable explants and the best hormone combination to optimize micropropagation of linden in vitro. Explants were collected and cultured randomly from the best individual of linden from Gorgan Touskaestan forest. Findings: In light of results, from the eleven tested explants, stem containing terminal buds with scale were selected as the most suitable explants and IBA 0.1 mg/l was selected as the most appropriate medium hormonal combination in shoot regeneration. Discussion and Conclusion: Obtained data were analyzed with a completely randomized design by MSTATC software. It is hoped that the achieved results can be reproduced and be helpful in tissue culture research and also the molecular basis studies

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