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Open Access Article
1 - Application of response surface methodology (RSM) for the optimization of supercritical CO2 extraction of Elaeagnus protein: a comparison study
Sepideh Hesamizadeh S. Askari محمدرضا حسین پورElaeagnus contains a large amount of protein which can be used as a functional ingredient. In this study, dried fruit protein was extracted using supercritical carbon dioxide method. Extraction was performed at different supercritical temperatures, supercritical pressur MoreElaeagnus contains a large amount of protein which can be used as a functional ingredient. In this study, dried fruit protein was extracted using supercritical carbon dioxide method. Extraction was performed at different supercritical temperatures, supercritical pressure, and solvent ratio of carbon dioxide and methanol. According to the ANOVA results, the optimum operating condition for the extraction of protein with supercritical CO2 was as following:150.85 bar, 51.06°C, 59.80 min and methanol aqueous solvent concentration of 717.08 µL, leading to extracting of 47.53 mg protein per 10 g sample. Compared to other extraction methods, the amount of extracted protein was high with this method. In addition, less damage was done to other physical-chemical properties of the protein, including protein structure, amino acid chains, etc. Among the operating conditions, supercritical pressure had the highest effect on proteins. Therefore, the results of this study suggested that the supercritical carbon dioxide extraction can be sued as an effective method for protein extraction from plants. Manuscript profile -
Open Access Article
2 - Optimization of Fortified Dough Composition for Spaghetti Production using Strong Wheat Flour
F. Ardestani S. E. Hosseini -
Open Access Article
3 - Antimicrobial Peptides Derived from Goat’s Milk Whey Proteins Obtained by Enzymatic Hydrolysis
M. Esmaeilpour M. R. Ehsani M. Aminlari Sh. Shekarforoush E. Hoseini -
Open Access Article
4 - Evaluation of Embryonic Development in Yellow Ground Squirrels (Spermophilus fulvus) and Electrophoresis Comparison of Serum and Cytogenetic Proteins of TheseSquirrels with Gray Squirrels (Spermophilus xanthoprymnus) (Mammalia: Rodentia) in Iran
mohammad Moradi GharkhlooInroduction & Objective: Among the squirrel family (Sciuridae), the yellow ground squirrel (Spermophilus fulvus) has the longest summer sleep. Summer sleep of these squirrels begins in late winter and continues until late February. The aim of the present study was E MoreInroduction & Objective: Among the squirrel family (Sciuridae), the yellow ground squirrel (Spermophilus fulvus) has the longest summer sleep. Summer sleep of these squirrels begins in late winter and continues until late February. The aim of the present study was Evaluation of Embryonic Development in Yellow Ground Squirrels (Spermophilus fulvus) and Electrophoresis Comparison of Serum and Cytogenetic Proteins of TheseSquirrels with Gray Squirrels (Spermophilus xanthoprymnus) (Mammalia: Rodentia) in Iran. Material and Method: In this project, a large number of babies were collected from several nests in May, and their development was examined in the laboratory.In this way, it was found that these squirrels give birth once a year, with 5-7 babies per calving.48 squirrels (28 males and 20 females) were collected from northeastern Iran (Mashhad and Bojnourd), western Iran (Kurdistan, Hamedan, Qazvin, and Zanjan), and northeastern Iran (Pīr Aḩmad Kandī, Kelisay-e Kandy, and Nadu village) to identify the species of ground squirrel (Spermphilus).The globulin and albumin proteins of all samples were first tested using the SDP-PAGE technique. Results: According to the results, in the sedimentary range of globulin proteins (G = Globulin), the samples collected from northeastern and western Iran formed 5 electrophoretic bands and those collected from northwestern Iran formed 11 electrophoretic bands.Besides, in the sedimentary range of albumin proteins (A = Albumin), the samples collected from northeastern and western Iran formed only one electrophoresis band and those collected from northwestern Iran formed 4 electrophoretic bands.During the karyological studies according to the method proposed by Ford and Hamerton, karyotypes were obtained from bone marrow cells to confirm the results of electrophoresis studies on the distribution of two of these squirrels in the collection areas in Iran.The results indicated that the karyotype of the samples collected from northeastern Iran (Mashhad) and western Iran (Qazvin, Hamedan, Zanjan, and Kurdistan) had 36 diploid chromosomes (2n = 36) and that the karyotype of the samples collected from northwestern Iran (Mako) had 42 diploid chromosomes (2n = 42). Conclusion: According to the results of electrophoresis and karyological methods, among ground squirrels, Sprmophilus fulvus species are distributed in northeastern and western Iran and Spermophilus xanthoprymnus species are distributed in northwestern Iran. Manuscript profile -
Open Access Article
5 - Effect of a session training on serum concentration of immunoglobulin A, cortisol and lipoprotein in the morning and the afternoon in elite male Taekwondo
Mohammad Hassan Boostani Mohammad Ali Boostani Fateme Khodaie Khodaie -
Open Access Article
6 - Biochemical basis of Benzothiadiazole-mediated defense in quince under orchard condition
Parinaz Etesamzadeh Mansoureh Keshavari Adeleh Sobhanipour Esfandiar ZohourBased on the recent results on the efficacy of Bion elicitor in increasing quince (Cydonia oblanga) resistance to fire blight caused by Erwinia amylovora bacterium, the biochemical basis of this induced defense was studied for the first time. The experiment was conducte MoreBased on the recent results on the efficacy of Bion elicitor in increasing quince (Cydonia oblanga) resistance to fire blight caused by Erwinia amylovora bacterium, the biochemical basis of this induced defense was studied for the first time. The experiment was conducted on quince cv. Isfahan seedlings grafted on quince rootstocks under orchard condition. In early spring, three-years-old young plants were sprayed twice with Bion (400 mg/l) in 4-days intervals and four days after the last spray, young leaves were collected for enzymes extraction and quantification. Also, phytotoxic effects of Bion were studied throughout the time course of the study in orchard. Based on the results, Bion did not induce any phytotoxicity effects on quince. Bion treatment increased polyphenol oxidase and peroxidase activities but did not affect on catalase activity. It is concluded that elevation of oxidative enzymes are a part of biochemical mechanism of Bion-mediated defense in quince but catalase enzyme activity, which dismutase reactive oxygen species, does not change. Manuscript profile -
Open Access Article
7 - QSAR, Molecular docking and Molecular dynamics studies simulation of Epigenetic inhibitors
ghasem ghasemi babak motahary Robabe SayadikordAbadi omid alizadehDevelopment of QSAR and molecular docking is a key to the elucidation of pathomechanisms of epigenetic diseases. Quantitative structure- activity relationship (QSAR), Molecular docking and molecular dynamics simulation were carried out for some modulators of modified ch MoreDevelopment of QSAR and molecular docking is a key to the elucidation of pathomechanisms of epigenetic diseases. Quantitative structure- activity relationship (QSAR), Molecular docking and molecular dynamics simulation were carried out for some modulators of modified chromatin proteins as anticancer agents. The Imperialist Competitive Algorithm (ICA), partial least squares (PLS), Principle component regression(PCR), Coralsea,and Multiple Linear Regression (MLR) were used to achieve the QSAR models. Suitable descriptors were selected which includes features such as atomic mass, Van der waals volume, shape and geometrical structure of compounds. Then, molecular docking studies were performed using Autodock Vina software which had a high throughput accuracy. Based on features such as number of hydrogen bonds, bonding length, binding affinity, and also root-mean-square deviation (RMSD), the best complex were selected. In general, QSAR, molecular docking and molecular dynamics simulation illustrated that compounds 9 and 14 were selected as suitable agents for the design of anticancer drugs. Drug-likeness descriptors of compounds calculated by DruLiTo. In the molecular docking study, the maximum binding affinity of -9 kcal/mol was obtained between each of enzyme systems (PDB: 3MXF) and the geometric-optimized molecules, representing a strong interaction. Manuscript profile -
Open Access Article
8 - A review of the nutritional and medicinal potential of Phycobiliproteins extracted from cyanobacteria
Bahareh Nowruzi yasaman GoraniPhycobiliproteins (PBPs) are colored and water-soluble biliproteins that are found in cyanobacteria and rhodophytes. Based on their spectral characteristics, PBPs are divided into three types: Allophycocyanin, phycocyanin and phycoerythrin. PBPs, apart from their specia MorePhycobiliproteins (PBPs) are colored and water-soluble biliproteins that are found in cyanobacteria and rhodophytes. Based on their spectral characteristics, PBPs are divided into three types: Allophycocyanin, phycocyanin and phycoerythrin. PBPs, apart from their special function as sunlight-receiving antennas in the photosynthesis process, can be used as food dyes, nutrients, cosmetics, pharmaceutical industries and fluorescent probes in immunofluorescence analysis. Since PBPs have antioxidant, anti-tumor effects, as well as potential anti-inflammatory and anti-diabetic properties, in this review article, an attempt was made to investigate the properties and medicinal potential of PBPs along with their structural features. The results of the review of recent articles showed that the PBP part of proteins is very sensitive to environmental stress and this issue limits their use in the food industry. Therefore, it is necessary to use protective and coating materials to preserve the color. Consequently, they can prevent the denaturation of the protein structure, which not only increases the antioxidant properties but also increases the half-life of the food. Manuscript profile -
Open Access Article
9 - Alterations in acute phase proteins, hemogram and electrophoretic pattern of equine serum proteins following change of diet from forage to forage mixed with concentrate
Rouhoullah Karampour Mohammad Razi Jalali mohammad rahim haji hajikalaei Alireza Ghadrdan mashhadiDespite extensive studies on the relationship between diet type and its effect on equine biochemical factors, little is known about horse serum and plasma proteins following changes in diet from forage to forage mixed with concentrate. Acute phase proteins are a group o MoreDespite extensive studies on the relationship between diet type and its effect on equine biochemical factors, little is known about horse serum and plasma proteins following changes in diet from forage to forage mixed with concentrate. Acute phase proteins are a group of serum proteins whose concentrations increase in response to infections, inflammation, trauma, and neoplasia. Therefore, in the present study, we evaluated the serum levels of serum biomarkers, fibrinogen and total protein concentration along with the nutritional behavior of horses during diet change. For this purpose, two types of diets, including forage (first treatment) and mixture of forage with concentrate (second treatment) were used for each horse for fifteen consecutive days. Complete blood cell counts as well as serum levels of fibrinogen, haptoglobin and amyloid type A were measured before and after dietary changes. Serum fibrinogen, haptoglobin and amyloid A levels were significantly increased in horses of the second treatment group compared to horses in the first treatment group (p<0.05). There was also a statistically significant difference in the complete blood cell count due to the change of diet from forage to forage mixed with concentrate (p<0.05). It seems that changes in the natural flora of the horse's digestive tract following a change in diet can protect the animal's body against acute inflammatory and traumatic injuries. Manuscript profile -
Open Access Article
10 - Comparative analysis of seed proteins in 10 cultivated genotypes (Amygdalus dulcis (L.) Miller) and 2 wild almonds (A. scoparia Spach & A. lycioides Spach) in Esfahan province
مهدی Yousefi,Seed protein analysis was performed among 10 cultivated genotypes (Amygdalus dulcis (L.) Miller genotypes: Moheb Ali, Safari, Yarollahi, Mamaei, Rabee, Kababi, Tageri, Hag Mirzaei, Talkheh and Azar) and 2 wild species of almond (Amygdalus scoparia Spach & A. lycioid MoreSeed protein analysis was performed among 10 cultivated genotypes (Amygdalus dulcis (L.) Miller genotypes: Moheb Ali, Safari, Yarollahi, Mamaei, Rabee, Kababi, Tageri, Hag Mirzaei, Talkheh and Azar) and 2 wild species of almond (Amygdalus scoparia Spach & A. lycioides Spach var. horrida (Spach) Browicz), from Esfahan province, in order to illustrate their interrelationships. All together, 18 protein bands were obtained, some of which were common in all species and cultivated genotypes. Some bands were occurred only in a single cultivated genotype or species, while, some others occurred in all local genotypes, but not in the wild species. The obtained data were analyzed through cluster analysis via UPGMA method and Euclidean distance coefficient, and through Principle Components Analysis (PCA). The results revealed that some local cultivated genotypes were similar, such as a close relationship was detected among the genotypes Safari, Yarollahi and Mamaei, as well as among Tageri, Hag Mirzaei and Kababi. Different patterns of protein bands were also observed between the genotypes and the wild species. As a result, the role of seed protein criteria in the genetic variations among studied genotypes and wild almond was discussed. Manuscript profile -
Open Access Article
11 - Some Physiological Responses of Nostoc sp. JAH 109 to the Combination Effects of Limited Irradiance, pH and DIC Availability
shadman Shokravi Fariba Amirlatifi maryam Safaie ن Ghasemi neda SoltaniGrowth, pigment compositions, nitrogenase activity, photosynthesis and heterosyct frequency fluctuations of dominant species Nostoc sp. JAH 109, isolated from rice field, were evaluated in combination of limited irradiance (2 uE.m-2.s-1), different pHs (5,7,9) and inorg MoreGrowth, pigment compositions, nitrogenase activity, photosynthesis and heterosyct frequency fluctuations of dominant species Nostoc sp. JAH 109, isolated from rice field, were evaluated in combination of limited irradiance (2 uE.m-2.s-1), different pHs (5,7,9) and inorganic carbon availability. Nostoc sp. JAH 109, can be considered as an alkalophilic organism. Optimal growth rate were observed at pH 9. Size of phycobilisomes and relationship between photosystem II and photosystem I increased in pH 9 and DIC available condition. This strain could not grow well in acidic condition, but neutral and alkaline condition cause active carbon dioxide concentration mechanism system. The pattern of nitrogenase activity seems more or less regular and linear at the first days after inoculation both in neutral and alkaline conditions. With respect to nitrogenase activity, the highest rate was in pH 9 and DIC availability. This seems true for heterocyst frequency fluctuations too. The higher photosynthetic capacity (Pmax) per unit of chlorophyll was resulted in higher DIC concentration at alkaline condition. Manuscript profile -
Open Access Article
12 - Spermatozoa Molecules in Relation to Bulls Fertility
اس.آ. لون ر. سینها آ. رحیم ب.آ. جانایی آ. سینق ن. شاه -
Open Access Article
13 - Abiotic stress trigger mitochondrial defense system: A comprehensive review.
Khalil saad-Allah Ibrahim Abdelsalam -
Open Access Article
14 - Investigation of Genetic Variations among Crested Wheatgrass Species Base of Agronomical Traits and Total Leaf Protein
Parvin Salehi Shanjani Ali Ashraf Jafari Razieh Jahanbaz -
Open Access Article
15 - Effects of Selection on Genetic Parameters of Secale montanum Based on Seed Storage Protein Marker
Parvin Salehi Shanjani Ali Ashraf Jafari Roya Hoseinzadeh -
Open Access Article
16 - Study of the Diversity in Different Cultivars of Pistacia vera L. Resistant to Drought and Salinity: Comparing Protein Patterns Using SDS-PAGE Method
R. Atatashafrooz S. M. R. Khoshru A. Tajabadipour -
Open Access Article
17 - Isolation of β-1 and 3-Gloconase from Medicago truncatula, exspress them in E.coli BL21 and investigation of their fungicide
N. Nabavi B. Najmi M. RasooliPathogenesis related proteins are compounds that produced by response to pathogens or environmental stress. Up to now numerous kind of β-1 and 3-Glucanase are identified in various plants and thein fungicide effect were tested. The aim of this research was isolatio MorePathogenesis related proteins are compounds that produced by response to pathogens or environmental stress. Up to now numerous kind of β-1 and 3-Glucanase are identified in various plants and thein fungicide effect were tested. The aim of this research was isolation, over-expression and analysis of physiological activities of β-1 and 3-Glucanase (PR-2) from Medicago truncatula. In this case by Medicago sativa and Pisum sativum β-1 and 3-Glucanase gene alignment on Medicago truncatula genome three open reading frame with %50 similarity by having start and stop codon were identified and isolated that fragments were cloned in PET21c vector and expressed in E.coli BL21. SDS PAGE technique reveal that proteins were produce as an inclusion bodies. We also test their fungicide effect after folding them to achieve their natural structure on Alternaria alternaria and Fusarium graminearum. The result were significant ( P< 0.05) by SAS system analysis. Manuscript profile -
Open Access Article
18 - The Effect of Recombinant Killer Factor (LF) and Protective Factor (mPA) Proteins on Human Cancer Cells
Mahboubeh Gholami Majid Moghbeli Farshid Kafilzadeh Mohammad Kargar Maryam Bikhof TorbatiCancer causes about 13% of human deaths and has not yet been properly treated. Conventional cancer treatment methods such as surgery, chemotherapy, and radiotherapy have many side effects and in some cases have no effect on cancer treatment, so creating appropriate and MoreCancer causes about 13% of human deaths and has not yet been properly treated. Conventional cancer treatment methods such as surgery, chemotherapy, and radiotherapy have many side effects and in some cases have no effect on cancer treatment, so creating appropriate and targeted therapies can revolutionize cancer treatment. New methods include gene therapy and using immunotoxins, which can act up to a high percentage. This study aimed to investigate the effect of modified PA protein that binds only to cancer cells along with lethal factor (LF) on cancer cells. In this regard, recombinant mPA and LF proteins were isolated from recombinant Bacillus subtilis and after examining the presence of protein bands and their concentrations using SDS-PAGE and Bradford methods, respectively, the combined effect of different concentrations of these proteins on lung cancer cells, Breast, prostate and pancreas were examined by MTT method. The results showed that 75 ng of recombinant LF protein and 50 ng of recombinant mPA protein had the greatest effect on cancer cell death and caused the death of more than 98% of the studied cancer cells. With these results, it is hoped that a new dimension can be added to cancer treatment by producing targeted toxins such as mPA and LF. Manuscript profile -
Open Access Article
19 - Determination of the diagnostic value of positive and negative acute phase proteins of the milk as new and reliable biomarkers in bovine subclinical mastitis
S.H ShiraziBeheshtiha, V. Rabbani شهاب الدین Safi , M Bolourchi مهرداد Ameri,Currently, somatic cell count (SCC) and bacterial culture is considered as the golden and ultimate standard methods for diagnosis of bovine subclinical mastitis. However, SCC has a low diagnostic accuracy. Therefore, foridentifcation of infected animals new biomarkers w MoreCurrently, somatic cell count (SCC) and bacterial culture is considered as the golden and ultimate standard methods for diagnosis of bovine subclinical mastitis. However, SCC has a low diagnostic accuracy. Therefore, foridentifcation of infected animals new biomarkers with high diagnostic accuracy are needed. Acute phase proteins(APPs) are proteins that are increased (positive APPs) or decreased (negative APPs) in response to inflammation.The objective of this study was to determine the diagnostic value of the milk APPs for the diagnosis of subclinicalmastitis in dairy cows. A total of 90 clinically healthy cows were randomly selected. Of these, 52 cows were considered to have subclinical mastitis based on a SCC higher than 130 × 1000 cells/mL of milk and positive bacterialculture results of milk samples obtained from at least one of the quarters. Milk amyloid A (MAA) concentrationwas measured using a commercial ELISA kit and albumin, α-lactalbumin, β-lactoglobulin, and immunoglobulin(Ig) were measured in whey samples by using cellulose acetate electrophoresis. Diagnostic sensitivity and specifcity and cutoff points for each test were determined via receiver-operating characteristics (ROC) analysis. Signifcant (P < 0.001) increases in the mean and median concentration of MAA, albumin, α-lactalbumin, and Ig werefound in the milk samples collected from cows with subclinical mastitis. MAA was the most accurate test witha diagnostic sensitivity of 92.3% and specifcity of 92.1% at cutoff point of > 1.6 mg/L. The results of this studyshowed that determination of MAA and some milk serum proteins such as albumin and immunoglobulins can beused as potential and reliable biomarkers for the diagnosis of bovine subclinical mastitis. Manuscript profile -
Open Access Article
20 - Genetic diversity of some wheat landraces of Zanjan province by gliadin patterns
Arash Mohammadi Mostafa Valizadeh Mohammad Moghaddam Yusef ArshadIn present research, genetic diversity of gliadins among 30 wheat landraces belong to region of Zanjan-Iran, was studied by Acid-PAGE technique. Totally 54 polymorphic gliadin bands were identified. The highest frequency was related to gliadin band 46 witch observed in MoreIn present research, genetic diversity of gliadins among 30 wheat landraces belong to region of Zanjan-Iran, was studied by Acid-PAGE technique. Totally 54 polymorphic gliadin bands were identified. The highest frequency was related to gliadin band 46 witch observed in 90% of wheats. Also gliadins 12.5 and 15.5 were observed in 86.7% of the wheats. Genetic diversity of the wheats based on total gliadin found to be high (H=0.967). Highest and Lowest genetic variation related to omega-gliadins (H=0.917) and gamma-gliadins (H=0.781) respectively. Wheats of Zanjan showed diversity in terms of gliadin bands number (11-25 bands). The entire wheats, showed specific gliadin pattern. Totally, 29 omega-gliadin patterns, 15 gamma-gliadin patterns, 22 beta-gliadin patterns and 23 alpha-gliadin patterns were observed. Cluster analysis could separate three tetraploid genotypes TN-11372, TN-11734, TN-11743 from hexaploids. These results indicate that gliadin proteins analysis is useful for evaluating of genetic diversity, characterizing of wheat genotypes and separating of different ploidy levels. Manuscript profile
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