Alterations in acute phase proteins, hemogram and electrophoretic pattern of equine serum proteins following change of diet from forage to forage mixed with concentrate

Subject Areas : Veterinary Clinical Pathology

Rouhoullah Karampour ¹ , Mohammad Razi Jalali ² , mohammad rahim haji hajikalaei ³ , Alireza Ghadrdan mashhadi ⁴

1 - PhD of Clinical Pathology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
2 - Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
3 - Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
4 - Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

Received: 2021-01-24 Accepted : 2021-06-21 Published : 2021-04-21

Keywords: Inflammation, Ahvaz, concentrate, Trauma, Karampour, R.1*, Razi Jalali, M.2, Haji Hajikolaei, M.R.3, Ghadrdan Mashhadi, Clinical Pathology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Iran. 2- Professor, Department of Clinical Sciences, Iran. 3- Professor, Iran. 4- Professor, little is known about horse serum and plasma proteins following changes in diet from forage to forage mixed with concentrate. Acute phase proteins are a group of serum proteins whose concentrations increase in response to infections, and neoplasia. Therefore, in the present study, we evaluated the serum levels of serum biomarkers, fibrinogen and total protein concentration along with the nutritional behavior of horses during diet change. For this purpose, two types of diets, including forage (first treatment) and mixture of forage with concentrate (second treatment) were used for each horse for fifteen consecutive days. Complete blood cell counts as well as serum levels of fibrinogen, haptoglobin and amyloid type A were measured before and after dietary changes. Serum fibrinogen, haptoglobin and amyloid A levels were significantly increased in horses of the second treatment group compared to horses in the first treatment group (p<0.05). There was also a statistically significant difference in the complete blood cell count due, Forage Horse, A.4 1- PhD, Iran. *Corresponding author's email: [e-mail] (Received: 2021/1/24 Accepted: 2021/6/21) Abstract Despite extensive studies on the relationship between diet type and its effect on equine biochemical factors,

Abstract :

Despite extensive studies on the relationship between diet type and its effect on equine biochemical factors, little is known about horse serum and plasma proteins following changes in diet from forage to forage mixed with concentrate. Acute phase proteins are a group of serum proteins whose concentrations increase in response to infections, inflammation, trauma, and neoplasia. Therefore, in the present study, we evaluated the serum levels of serum biomarkers, fibrinogen and total protein concentration along with the nutritional behavior of horses during diet change. For this purpose, two types of diets, including forage (first treatment) and mixture of forage with concentrate (second treatment) were used for each horse for fifteen consecutive days. Complete blood cell counts as well as serum levels of fibrinogen, haptoglobin and amyloid type A were measured before and after dietary changes. Serum fibrinogen, haptoglobin and amyloid A levels were significantly increased in horses of the second treatment group compared to horses in the first treatment group (p<0.05). There was also a statistically significant difference in the complete blood cell count due to the change of diet from forage to forage mixed with concentrate (p<0.05). It seems that changes in the natural flora of the horse's digestive tract following a change in diet can protect the animal's body against acute inflammatory and traumatic injuries.

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Study of changes in acute phase proteins of serum, hemogram and electrophoretic pattern of horse serum proteins following change of diet from forage to forage mixed with concentrate.