Comparative analysis of seed proteins in 10 cultivated genotypes (Amygdalus dulcis (L.) Miller) and 2 wild almonds (A. scoparia Spach & A. lycioides Spach) in Esfahan province

Subject Areas : Genetic

مهدی Yousefi, ¹ ( Department of Biology, Payam Noor University, Esfahan, Iran )

Received: 2008-03-03 Accepted : 2008-06-14 Published : 2008-12-21

Keywords: electrophoresis, seed proteins, Genotypes, Esfahan Province, Almond (Amygdalus dulcis),

Abstract :

Seed protein analysis was performed among 10 cultivated genotypes (Amygdalus dulcis (L.) Miller genotypes: Moheb Ali, Safari, Yarollahi, Mamaei, Rabee, Kababi, Tageri, Hag Mirzaei, Talkheh and Azar) and 2 wild species of almond (Amygdalus scoparia Spach & A. lycioides Spach var. horrida (Spach) Browicz), from Esfahan province, in order to illustrate their interrelationships. All together, 18 protein bands were obtained, some of which were common in all species and cultivated genotypes. Some bands were occurred only in a single cultivated genotype or species, while, some others occurred in all local genotypes, but not in the wild species. The obtained data were analyzed through cluster analysis via UPGMA method and Euclidean distance coefficient, and through Principle Components Analysis (PCA). The results revealed that some local cultivated genotypes were similar, such as a close relationship was detected among the genotypes Safari, Yarollahi and Mamaei, as well as among Tageri, Hag Mirzaei and Kababi. Different patterns of protein bands were also observed between the genotypes and the wild species. As a result, the role of seed protein criteria in the genetic variations among studied genotypes and wild almond was discussed.

References:

اداره کل آمار و اطلاعات وزارت جهاد کشاورزی (1377). خشکبار، آمار و مرایا. انتشارات وزارت جهاد کشاورزی، صفحات 101 تا 153.

خاتمساز، م. (1370). تیره گل سرخ (Rosaceae)، فلور ایران. شماره 6، انتشارات مؤسسه تحقیقات جنگلها و مراتع، صفحات 274 تا 315.

 

 

عالم، ف.ز. (1385). بررسی تاکسونومیکی گونه‌های وحشی و کولتیوارهای زراعی بادام در استانهای اصفهان و چهار محال و بختیاری، پایان‌نامه کارشناسی ارشد، دانشگاه پیام نور، مرکز تهران، 70 صفحه.

فیضی، م.ت. (1380). معرفی بادام کوهی (Amygdalus scoparia) و برخی ویژگی‌های اکولوژیکی آن در استان اصفهان، مجموعه مقالات دهمین کنفرانس سراسری زیست‌شناسی ایران، صفحات 477 تا 480.

Andrews, A.T. (1993). Electrophoresis, theory, techniques and biochemical and clinical applications, Claredon Press, Oxford.

Barbera, G., Marco, L. and Schirra, M. (1994). Effects of rootstock on productive and qualitative response of two almond varieties, Acta Horticulturae, 373: 129- 134.

Bortiris, S. O., Sang-Hun, J. J., Beggtt, S., Granger, A., Weeks, C., Buckinghan, M., Potter, D., and Parfitt, D. (2001). Phylogenetic and systematics of Prunus (Rosaceae) as determined by sequence analysis of its and the chloroplast trn-trnf spacer DNA, Systematic Botany, 26(4): 797- 804.

Browicz, K. (1969).  Amygdalus. L. In: K. H. Rechinger (ed.), Flora Iranica, 66: 166- 187, Graz., Austria.

Browicz, K. and Zohary, D. (1996). the genus Amygdalus L. (Rosaceac): species relationship, distribution and evolution under domestication, Genet. Resour. Crop Evol. 43: 229- 247.

Buth, D. G. (1984). The application of electrophoretic data in systematic studies, Ann. Ecol. Syst. 15: 501- 522.

Cook, R. T. (1984). The characterization and identification. of crop cultivars by electrophoresis, Electrophoresis, 5:59- 72.

Dowidar, A. E., Kamel, E. A., Ahmed, A. M., Loutfy, M. H. A. and Hafez, H. H. L. (2003). Studies on Rosaceae II- SDS-PAGE seed protein electrophoresis and its significance in the taxonomy of the family, Pakistan Journal of Biological sciences, 6 (21): 1820- 1829.

Esteban, R. M. and Lopez, A. F. J. (1985). Protein extractability of almond (Prunus amygdalus) seeds, Journal of the Science of Food and Agriculture, 36(6): 485- 490.

FAO, (2002). FAO statistical databases: Agriculture: http:// apps.fao.org.

Ladizinsky, G. (1983). Study of evolution problems by means of seed proteins. In: Seed proteins, biochemistry, genetics, nutritive value. (W. Gohschalk and H.P. Muller. eds.) pp. 481-498. Mortinus Nighoff. Dr. W. Junk Publishers, The Hagues, London.

Ladizinsky, G. (1999). On the origin of almond, Genet. Resources Crop Evol. 46: 143-147.

Ladizinsky, G. and Hymowitz, T. (1979). Seed protein electrophoresis in taxonomic and evolutionary studies, Theo. App. Genet., 54: 145-151.

Mowery, B. D. and Werner, D. J. (1990). Phylogenetic relationship among species of Prunus as inferred by isozyme markers, Theoritical and Applied Genetics, 80(1): 133-189.

Rahemi, A. R. (2002). The development of almond orchards in Iran, Acta Hort. (SHS), 591:177-180.

Sathe, S.K., Teaber, S.S., Gradziel, T.M. and Roux, K.H. (2001). Electrophoretic and immunological analysis of almond (Prunus dulcis L.) genotypes and hybrids, J. Agr. Food. Chem. 49: 2043-2052.

Saura-Calixto, F., Bauza, M., Martinez, D. F. and Argamenteria, A. (1981). Aminoacids, sugars and inorganic elements in the sweet almond (Prunus amygdalus), J. Agric. Food. Chem. 29: 509-511.

Sheidai, M., Hamta, A., Jaffari., A. and Noori Dalooi, M. R. (1999). Morphometric and seed protein studies of Trifolium species and cultivars in Iran, Plant Genet. Res. Newsl. , 120: 52-54.

Siami, A., Heidari, R. and Mohseni, M. (2002). Comparative study of amygdaline, fat and total protein of 7 species of wild almond in west Azarbaidjan (Iran), Acta Hort. (ISHS), 591:181-187.

Statsoft, Inc. (199). STATISTICA for Windows, Version 4.5.

Talaei, A. and Imani, A. (1998). Morphology of pollen grains as an index for identification of local Iranian almond varieties, Acta Hort. (ISHS), 470: 280-285.

Vezvaei, A. (2003). Isozyme diversity in Iranian almond, Proc. XXVT Acta Hort. (ISHS), 622: 451-456.