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Open Access Article
1 - Enzyme Inhibitory Activity of Certain Vegetables Indigenous in Iran as Potential Antiandrogens
A. Sharifan A. Hajhoseini M. Bakhtiari -
Open Access Article
2 - The evaluation of carbohydrate, phenol compounds changes and anti-oxidant enzymes activity during the release of dormancy in Persian walnut lateral buds by different chilling treatments
ژیلا Gholizadeh حمیدرضا صادقی پور احمد عبدل زاده خدایار همتیThe purpose of this study was assessing the impacts of different chilling periods on the release of dormancy in walnut lateral buds, carbohydrates metabolism, phenol compounds content, and activities of soluble and cell wall peroxidase and polyphenol oxidase. In this li MoreThe purpose of this study was assessing the impacts of different chilling periods on the release of dormancy in walnut lateral buds, carbohydrates metabolism, phenol compounds content, and activities of soluble and cell wall peroxidase and polyphenol oxidase. In this line, one-year-old shoots were collected randomly from ten-year-old trees of Ziyarat village (Gorgan) in defoliation which were then cut into 9 cm pieces each with a single lateral bud and were subjected to different chilling treatments (0, 700, 1400 hour) at 4±1 °C and then forcing condition (25 ± 2 ◦C, 12 h light:12 h dark photoperiod, 20 µmol m−2 s−1). Results showed that 700 and 1400 chilling hours significantly increased percentage and rate of walnut lateral bud dormancy release and decreased time of initiation bud break in comparison with control. Chilling caused starch and non-reducing sugar degradation and resulted in the increase of reducing sugars. This had a positive significant relationship with the percentage of bud dormancy release. Phenols content, soluble and cell wall peroxidase activities increased up to 700 hour chilling while polyphenol oxidase activity increased up to 1400 hour as compared with control. It seems that the buildup of reducing sugars provides the required energy for dormancy release, and the increase of phenols and soluble and cell wall peroxidase activities result in decrease of oxidative stress and development of cold tolerance in buds. Moreover, rising polyphenol oxidase activity caused removing phenolic inhibitors and/or synthesis of antioxidant phenols. These processes in parallel promoted dormancy release of Persian walnut lateral buds. Manuscript profile -
Open Access Article
3 - Study on antioxidant enzymes activity in active and banjhi buds of tea (Camellia sinensis L) cultivars (clone 100 and hybrid)
seyede mehri javadi mehri beigmohamadiThe yield and quality of tea depend on the shoot growth and bud dormancy. Tea (Camellia sinensis) is one of the natural sources of bioactive compounds, such as caffeine, catechins, and antioxidants, which is consumed as a “health drink” due to its beneficial MoreThe yield and quality of tea depend on the shoot growth and bud dormancy. Tea (Camellia sinensis) is one of the natural sources of bioactive compounds, such as caffeine, catechins, and antioxidants, which is consumed as a “health drink” due to its beneficial medicinal properties. The bud dormancy increases the distance of leaf plucking and reduces the yield of tea. In this study, activities of antioxidant enzymes were assessed in banjhi and active buds in two cultivars, clone 100 and hybrid. The results showed that there were significant differences in antioxidant enzyme activity, indicating genetic diversity between them. The activities of enzymes such as ascorbate peroxidase, catalase and peroxidase in the banjhi buds were higher than the active buds, however, in banjhi buds, the peroxidase activity was higher than the active budsThe yield and quality of tea, which is consumed as a “health drink”, depend on the shoot growth and bud dormancy. The bud dormancy increases the distance of leaf plucking and reduces the yield of tea. In this study, activities of antioxidant enzymes were assessed in banjhi and active buds in two cultivars, clone 100 and hybrid. The results showed that there were significant differences in antioxidant enzyme activity, indicating genetic diversity between them. The activities of enzymes such as ascorbate peroxidase, catalase and peroxidase in the banjhi buds were higher than the active buds, however, in banjhi buds, the peroxidase activity was higher than the active buds. According to the results, it seems that biochemical changes are involved in the development of banjhi bud and active bud and these changes can be used as a biochemical marker not only in detecting the yield of tea cultivars but also in reducing the dormant periods. Manuscript profile -
Open Access Article
4 - Removal of Malachite Green by Using Immobilized Glucose Oxidase Onto Silica Nanostructure-Coated Silver Metal-Foam
Mansour Hemmati Mohammad Barkhi Hadi Baharifar Kamyar Khoshnevisan -
Open Access Article
5 - Changes in some physiological traits and mucilage yield of sour tea (Hibiscus Sabdariffa L.) under foliar application of magnesium and iron oxide nanoparticles
Payam Moaveni Hasti Kiapour Behzad Sani faizeh rajabzadeh Hamid Mozafari -
Open Access Article
6 - Effects of sodium Nitroprussid and Calcium silicate on the physiological and biochemical parameters of wheat (Triticum aestivum L.) under cadmium stress
Saeid Soltani -
Open Access Article
7 - Static magnetic field in plants: physiological effects and antioxidant defense mechanisms-an overview
Halimeh Hassanpour Mahbobeh Ghanbarzadeh -
Open Access Article
8 - پاسخ مورفولوژیکی و فیزیولوژیکی شمشاد (Euonymus japonicas) به تیمار پاکلوبوترازول تحت تنش شوری
حمیمه زورمان مهناز کریمیپاکلوبوترازول یکی از مهمترین ترکیبات تریازولی می­باشد. این ماده تحمل گیاه را در برابر تنش­های محیطی افزایش میدهد. این مطالعه با هدف بررسی تأثیر پاکلوبوترازول بر صفات مورفولوژیکی و بیوشیمیایی شمشاد (Euonymus japonicas) تحت تنش شوری انجام گرفت. گیاهان ابتدا با پا Moreپاکلوبوترازول یکی از مهمترین ترکیبات تریازولی می­باشد. این ماده تحمل گیاه را در برابر تنش­های محیطی افزایش میدهد. این مطالعه با هدف بررسی تأثیر پاکلوبوترازول بر صفات مورفولوژیکی و بیوشیمیایی شمشاد (Euonymus japonicas) تحت تنش شوری انجام گرفت. گیاهان ابتدا با پاکلوبوترازول (0، 500 و 1000 میلی­گرم در لیتر) تیمار شدند و سپس تحت تنش شوری (0 ، 50 ، 100 ، 150 میلی­مولار) قرار گرفتند. خصوصیات مورفولوژیکی، نشت الکترولیت، محتوای کلروفیل و فعالیت آنزیم­های آنتی­اکسیدانی با تیمار پاکلوبوترازول و تحت تنش نمک تغییر یافت. در مقایسه با گیاهان شاهد، در 1000 و 500 میلی­گرم پاکلوبوترازول، به­ترتیب کاهش ارتفاع 8/22 و 44/10 درصد مشاهده شد. بیشترین تعداد شاخساره جانبی در 1000 میلی­گرم در لیتر پاکلوبوترازول بدون تنش شوری ثبت شد. اثر متقابل شوری و پاکلوبوترازول بر وزن تر اندام هوایی معنی­دار بود. در غلظت 150 میلی مولار نمک + 1000 میلی­گرم در لیتر پاکلوبوترازول ، وزن تر اندام هوایی 30٪ در مقایسه با 150 میلی­مولار نمک (بدون تیمار پاکلوبوترازول) افزایش نشان داد. در گیاهان تیمار شده با پاکلوبوترازول فعالیت آنزیم سوپراکسید دیسموتاز نسبت به گیاهان بدون تیمار با پاکلوبوترازول بیشتر بود. در مقایسه با 150 میلی­مولار کلرید سدیم (بدون تیمار پاکلوبوترازول)، هدایت الکتریکی 4/24 درصد، در 1000 میلی­گرم در لیتر پاکلوبوترازول + 150 میلی­مولار کلرید سدیم کاهش یافت. با توجه به نتایج این مطالعه می­توان بیان کرد که تیمار پاکلوبوترازول می­تواند تنش شوری (150 میلی مولار) را در گیاهان شمشاد کاهش دهد. Manuscript profile -
Open Access Article
9 - Molecular screening and of extracellular protease producing Serratia marcescens from the different environments of west Mazandaran
Raheleh Soltanmoradi Amir Salim Razmara Seyed Reza HosseinidoostBackground and Objectives: Proteases are a useful family of enzymes in microbiology with applications in leather industry, food industry, pharmacy, cleaners, and many other industries. Due to rapid replication, fast growing and facility in their genetic manipulations, m MoreBackground and Objectives: Proteases are a useful family of enzymes in microbiology with applications in leather industry, food industry, pharmacy, cleaners, and many other industries. Due to rapid replication, fast growing and facility in their genetic manipulations, microorganisms are used as the main resources of production of proteases. This paper aimed to isolate the Serratia marcescens strains from the natural environments at western Mazandaran, with the ability to produce proteolytic enzymes. Materials and Methods: In the present study, 150 natural samples were taken from different depth (0-10 cm) of soils and waters located at western part of Mazandaran. In order to isolate and to identify the microorganisms the samples were grown on Skim milk agar, and the isolates were underwent different biochemical tests. Next, Serratia marcescens strains were screened by molecular tests and 16SrRNA. After isolation of the strains, several time and temperature optimization steps were performed on the most potent enzyme producing strains. As well, approximate molecular weight of the enzymes were measured abased on protein deposition and SDS-PAGE. Results: Only two isolates were identified as Serratia marcescens. One of the two isolates belongs to a novel strain nominated as Serratia marcescens 424, which recorded in Genbank as KC790390. These two isolates could produce high levels of proteolytic enzyme in 24 hours under 37oC. The highest amount of enzyme belonged to a 52 Dalton molecular weight isolated from Serratia marcescens 424. Conclusion: Our studies show a high potential of the isolated Serratia marcescens strains in production of protease and Serratiopeptidase. As a result these strains can be useful in various industries. Manuscript profile -
Open Access Article
10 - Isolation and identification of native Iranian L-asparaginase producing bacteria
Roya Yazdani Mohsen Mobini-Dehkordi Ali Asghar Rastegari -
Open Access Article
11 - Evaluating Foliar Application of Calcium Nitrate, Calcium Chloride and Boric Acid on Physiological Disorders of ‘Kaleh - Ghoochi’ Pistachio
Ebrahim Pourahmadi Abdorahman Mohamadkhani Parto Roshandel Somayeh Momenyan -
Open Access Article
12 - Allelopathic Effects of Sorghum on Milk Thistle (Silybum marianum L.) Seed Germination and Growth
HOSSEIN MOKHTARI KARCHEGANI SEYEDEH ZAHRA HOSSEINI CICI SEYED ABDOLREZA KAZEMEINI -
Open Access Article
13 - The interaction study of hormonal priming and osmopriming on germination and biochemical indices of sainfoin
M. ousefi Rad M. Sharif Moghadasi A. Masomi Zavarian M. Asghari M. Nejati In order to interaction study hormonal priming and osmopriming on germination and biochemical indices sainfoin, an experiment was conducted as factorial based on randomized completely design with three replication. The first factor was thr More In order to interaction study hormonal priming and osmopriming on germination and biochemical indices sainfoin, an experiment was conducted as factorial based on randomized completely design with three replication. The first factor was three levels of non-consumption (control), 100 and 200 mg per liter consuming gibberellins and the second factor was non-consumption (control), consuming 5 and 10% polyethylene glycol. Based on the research results, pre-treatment of seeds with GA increased the percentage and rate of germination, root and shoot length, seedling dry weight, seed vigor, Amylase and peroxidase activity and the protein content. Average comparison results showed that amount of 100 mg per liter GA had the greatest influence. The results also showed that seed pre-treatment with polyethylene glycol (5%) had the best effect on measured traits and level 10% reduction root and shoot length and the protein content. In general, present research showed pre-treatment of sainfoin seeds with 100 mg per liter GA and 5% polyethylene glycol had greatest effect on germination and biochemical. Manuscript profile -
Open Access Article
14 - The effect of cold stress on the activity of malon di-aldehyde, peroxidase and catalase enzymes in several species of thyme
milad moshavegh saadollah houshmand Mohamad rabieiChilling stress is an abiotic stress including meteorological dynamics that happens at low temperatures. Chilling stress was introduced as the most important limiting factor in tropical and subtropical plants. In antioxidant activity, catalase, malon di-aldehyde and per MoreChilling stress is an abiotic stress including meteorological dynamics that happens at low temperatures. Chilling stress was introduced as the most important limiting factor in tropical and subtropical plants. In antioxidant activity, catalase, malon di-aldehyde and peroxidase enzymes were treated at temperatures of 20 ° C (control), zero degrees and 5 ° C in three Thymus vulgaris, Thymus daensis and Thymus kotschyanus thyme cultivars. The composite analysis was carried out with 3 replications. The results showed that there was a significant variation among the Thymus cultivars in terms of how to respond to temperature stresses, and the rate of enzyme changes in each of 3 significant factors justifying the role protecting them against environmental changes. Only in the amount of activity of catalase activity, the interaction of cultivars with treated temperatures was not particularly significant at 20 ° C (control) and zero degree, indicating the equality of the mode of reaction of thyme cultivars to the two treated temperatures. Manuscript profile