The interaction study of hormonal priming and osmopriming on germination and biochemical indices of sainfoin
Subject Areas : New Finding in Agriculture
M. ousefi Rad
1
(
Department of Agronomy, Faculty of Agriculture, Saveh Branch, Islamic Azad University, Saveh, Iran
)
M. Sharif Moghadasi
2
(
Department of Agronomy, Faculty of Agriculture, Saveh Branch, Islamic Azad University, Saveh, Iran
)
A. Masomi Zavarian
3
(
Young Researcher and Elite Club, Saveh Branch, Islamic Azad University, Saveh, Iran
)
M. Asghari
4
(
Department of Agronomy, Faculty of Agriculture, Saveh Branch, Islamic Azad University, Saveh, Iran
)
M. Nejati
5
(
Department of Agronomy, Faculty of Agriculture, Saveh Branch, Islamic Azad University, Saveh, Iran
)
Keywords: Protein, seed vigor, Germination percentage, Pre-treatment, Enzyme activity,
Abstract :
In order to interaction study hormonal priming and osmopriming on germination and biochemical indices sainfoin, an experiment was conducted as factorial based on randomized completely design with three replication. The first factor was three levels of non-consumption (control), 100 and 200 mg per liter consuming gibberellins and the second factor was non-consumption (control), consuming 5 and 10% polyethylene glycol. Based on the research results, pre-treatment of seeds with GA increased the percentage and rate of germination, root and shoot length, seedling dry weight, seed vigor, Amylase and peroxidase activity and the protein content. Average comparison results showed that amount of 100 mg per liter GA had the greatest influence. The results also showed that seed pre-treatment with polyethylene glycol (5%) had the best effect on measured traits and level 10% reduction root and shoot length and the protein content. In general, present research showed pre-treatment of sainfoin seeds with 100 mg per liter GA and 5% polyethylene glycol had greatest effect on germination and biochemical.
1- پرمون، ق.، عبادی، ع.، قویعزم، ع. و میری، م. 1392. اثر پیشتیمار بذر بر جوانهزنی و رشد گیاهچه بابونه در شرایط شوری. نشریه تولید گیاهان زراعی. 6 (3): 164-145.
2- رضایی سوخت آبندانی، ر.، محسنی، ا.، رمضانی، م. و مبصر، ح. ر. 1388. تأثیر پرایمینگ بر صفات جوانهزنی بذر ذرت K.SC 640. یافتههای نوین کشاورزی. سال چهارم. 1: 61-49.
3- زارع، م.، مهرابی اولادی، ع. ا. و شرفزاده، ش. 1385. بررسی اثرات اسید جیبرلیک و کینتین بر جوانهزنی و رشد گیاهچههای گندم تحت تنش شوری. مجله علمی پژوهشی علوم کشاورزی. 12 (4): 865-855.
4- عموآقایی، ر. 1383. تأثیر برخی تنظیمکنندگان رشد در تحریک جوانهزنی بذر کما (Ferula ovina). مجله پژوهشی علوم پایه دانشگاه اصفهان. 24 (2): 50-39.
5- عیسوند، ح. ر.، آذرنیا، م.، نظریان فیروزآبادی، ف. و شرفی، ر. 1390. بررسی اثر جیبرلین و اسید آبسزیک بر سبز شدن و برخی خصوصیات فیزیولوژیکی بذر و گیاهچه نخود در شرایط دیم و آبی.مجله علوم گیاهان زراعی ایران. 42 (4): 797-789.
6- فتحی، ق. و اسماعیلپور، ب. 1379. مواد تنظیمکننده رشد گیاهی اصول و کاربرد (ترجمه). انتشارات جهاد دانشگاهی مشهد.
7- مسرت، ن.، سیادت، ع.، شرفیزاده، م. و حبیبیخانیانی، ب. 1392. تأثیر هالوپرایمینگ و هیدروپرایمینگ بر جوانهزنی بذر و رشد اولیه گیاهچه ذرت رقم هیبرید SC704 تحت تنش شوری و خشکی. فصلنامه علمی پژوهشی فیزیولوژی گیاهان زراعی. 19: 59-49.
8- معصومی زواریان، ا.، یوسفی راد، م. و اصغری، م. 1394. اثر پیش تیمار سالیسیلیک اسید بر ویژگیهای جوانهزنی و بیوشیمیایی ماریتیغال (Silybum marianum L.) در شرایط تنش شوری. نشریه تحقیقات بذر، سال پنجم. 2: 48-40.
9- یداللهی نوشآبادی، س. ج. و شریفزاده، ف. 1394. اثر پیش تیمار بذور با جیبرلیک اسید بر شاخصهای جوانهزنی علف گندمی تحت تنش خشکی. صلنامه بومشناسی گیاهان زراعی. 11 (1): 82-75.
10- Abdul- Baki, A. and Anderson, J. D. 1973. Vigor determination in Soybean seed by multiple criteria. Crop Sci. 13: 630-633.
11- Ali, H. M., Siddiqui, M. H., Basalah, M. O., Al- Whaibi, M. H., Sakran, A. M. and Al-Amri, A. 2012. Effect of gibberellic acid on growth and photosynthetic pigments of Hibiscus sabdariffa L. African Journal of Biotechnology 11: 800-804.
12- Al-Rumaih M. M., Rushdy, S. S. and Warsym, A. S. 2002. Alteration in the protein Electrophoretic patterns of Cowpea, (Vigina unguiculata L.) Treated with Cadmium in the Presence or Absence of Gibberellic Acid. Saudi J. of Biol. Sci. 9: 47-56.
13- Ansari, O., Choghazardi, H. R., Sharif Zadeh, F. and Nazarli, H. 2012. Seed reserve utilization and seedling growth of treated seeds of mountain rye (Secale Montanum) as affected by drought stress. Cercetări Agronomice în Moldova. 2(150): 43-48.
14- Artola, A., Carrillo-Castaneda, G. and Santos, G. D. L. 2003. Hydro priming: a strategy to increase Lotus corniculatus L. seed vigor. Seed Science and Technology 31: 455-463.
15- Asghari, M., Eradatmand Asli, D., Yosefirad, M. and Ghandian, M. 2013. The effect of pyridoxine and its duration application on bioactive compounds and biochemical activities of germinated wheat. Ann. Biol. Res. 4 (3): 31-36.
16- Ashraf, M., Athar, H. R., Harris, P. J. C. and Kwon, T. R. 2008. Some prospective strategies for improving crop salt tolerance. Advanced in Agron. 97: 45-92.
17- Basra, S. M. A., Pannu, I. A. and Afzal, I. 2003. Evaluation of seedling vigour of hydro and matriprimed wheat (Triticum aestivum L.) seeds. Int. Agri. Biol. 5: 121-123.
18- Bradford, K. J. 1986. Manipulation of seed water relation via osmotic priming to improve germination under stress condition. Horticultural Science, 21, 1105-1112.
19- Bradford, K. J. 1995. Water relation in seed ge rmination. In: J. Kigel and G. Galili (eds), Seed development and germination. Marcel Dekker. Pp: 351- 396.
20- Bradford, M. 1976. A rapid and sensitive method for the quantitation of protein utilizing the principle of protein-dye binding. Annu. Rev. Biochem.72:248-254.
21- Dadkhah, M., Majidi, M. M. and Mirlohi, A. 2011. Multivariate analysis of relationships among different characters in Iranian Sainfoin populations (Onobrichis viciifolia Scop.). Iranian Journal of Field Crop Science, 42:349-357.
22- Delgado, I., Salvia, J., Buil, I. and Andres, C. 2008. The agronomic variability of a collection of sainfoin accessions. Spanish J Agric Res, 3, 401-407.
23- Demir, I. and Ellis, R. 1994. The effects of priming on germination and longevity of harvested pepper seed lots. Turkish Journal of Agriculture and Forestry. 18: 213-217.
24- Dominguez, F. and Cejudo, F. J. 1999. Patterns of starchy endosperm acidification and protease gene expression in wheat grains following germination. Plant Physiology, 119, 81-88.
25- Eisvand, H. R., Tavakkol-Afshari, R., Sharifzadeh, F., Madah Arefi, H. and Hesamzadeh Hejazi, S. M. 2008. Improvement of physiological quality of deteriorated tall wheat grass (Agropyron elongatum Host) seeds by hormonal priming for control and drought stress conditions. Iranian Journal of Field Crop Science, 1(39), 53-65. (In Farsi)
26- Ghana S. G. and Schillinger, W. F. 2003. Seed priming winter wheat for germination emergence, and yield, Crop Science. 43:2135-2141.
27- Ghiyasi, M., Pouryousef Myandoab, M., Tajbakhsh, M., Salehzade, H. and Meshkat, M. V. 2008. Influence of different osmopriming treatments on emergency and yield of Maize (Zea mays L.). Research Journal of Biological Sciences, 3, 1452-1455.
28- Ghorbani Javid, M., Sorooshzadeh, A., Moradi, F., Modarres Sanavy, S. A. M. and Allahdadi, I. 2011. The role of phytohormones in alleviating salt stress in crop plants. Australian Journal of Crop Science 5: 726-734.
29- Girolamo, G. D. and Barbanti, L. 2012. Treatment conditions and biochemical processes.
30- Haigh, A. M. and Barlow, E. W. R. 1986. Field emergence of tomato, carrot and onion seeds primed in an aerated salt solution. J. Amer. Soc. Hort. Sci.111:660-665.
31- Heidari Sharif Abad, H. and Dorry, M. A. 2003. Research institute of forest and rangelands. Forage Grasses Journal (in Persian with English abstract) 2: 311-320.
32- Jamil, M. and Rha, E. S. 2007. Gibberllic acid (GA3) enhance seed water uptake, germination and early seedling growth in sugar beet under salt stress. Pakistan Journal of Biological Sciences 10: 654-658.
33- Maguire, J. D. 1962. Speed of Germination-Aid in Selection and Evaluation for Seedling Emergence and Vigor. Crop Sci. 2: 176-177.
34- Masoumi Zavariyan, A., Yousefi Rad, M. and Asghari, M. 2015. Effect of seed priming by potassium nitrate on germination and biochemical indices in Silybum marianum L. under salinity stress. Int. J. Life Sci. 9(1): 23-29.
35- Michel, B. E. and Kaufmann, M. R. 1973. Theosmotic potential of polyethylene glycol 6000. Plant Physiol. 51: 914-916.
36- Nelson, C. P. 2000. Water potential: The key to successful seed priming. Decagon Devices, Inc. AN4101- 10.
37- Nickel, R. S. and Cunningham, B. A. 1969. Improved peroxidase assay method using leuco 2,3,6-trichloroindophenol and application to comparative measurements of peroxidase catalysis. Anal. Biochem. 27:292–299.
38- Paraoussi G., Voyiatzis P.G., Paroussis, E. and Drogoudi, P. D. 2002. Growth, flowering and yield responses to GA3 of strawberry grown under different environmental conditions. Scientia Horticulturae. 96: 103-113.
39- Permon, G. 2014. Effect of seed priming on chamomile germination and seedling growth at salinity conditions. Journal of crop production 6: 145-164.
40- Richards, D. E., King, K. E., Ait-ali, T. and Harberd, N. P. 2001. How gibberellin regulates plant growth and development: A molecular genetic analysis of gibberellin signaling. Annu. Rev. Plant Physiology. Plant Molcular Biology, 52: 67–88.
41- Tylkowska, K. and Van den Bulk, R. W. 2001. Effects of osmo and hydro priming on fungal infestation levels and germination of carrot (Daucus carota L.) seeds contaminated with Alternaria spp. Seed Science and Technology29: 365-375.
42- Windauer, L., Altuna, A. and Benech-Arnold, R. 2007. Hydrotime analysis of Lesquerella fendleri seed germination responses to priming treatments. Industrial Crops and Products, 25: 70–74.
43- Xiao. Z., Storms, R. and Tsang, A. 2006. A quantitative starch-iodine method for measuring alpha-amylase and glucoamylase activities. Analytical Biochemistry 351: 146-148.
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