Abortion causes significant economic losses to the livestock investment of a country and reduces fertility and production. Infectious agents are responsible a part of the sheep abortions and often, they are contagious and zoonosis, so they are also considered in terms o More
Abortion causes significant economic losses to the livestock investment of a country and reduces fertility and production. Infectious agents are responsible a part of the sheep abortions and often, they are contagious and zoonosis, so they are also considered in terms of public health. Campylobacter fetus and Leptospira introgans are some of the infectious agents of ovine abortion worldwide and economic and hygienic losses resulting from them are significant. Due to the importance of Campylobacterial and leptospiral sheep abortions, in this study 98 samples from abomasal contents of aborted sheep fetuses in Isfahan, Chaharmahal va Bakhtiari and Khorasan Razavi provinces were investigated for Campylobacter fetus subspecies fetus and Leptospira introgans by PCR.
Results showed that the infection with Campylobacter fetus in the samples studied was 4.9% and any samples weren’t found to be infected with Leptospira. The results of this study showed that campylobacter fetus might be involved in the incidence of a part of sheep abortions.
According to different diagnostic methods of the Leptospira, it seems that any diagnostic procedure has some weaknesses and using only a test is one can’t certainly report that the presence of this agent is negative. Therefore, it is recommended to several methods to be used simultaneously in order to compare the results of several methods and achieve accurate diagnosis.
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Campylobacter jejuni/coli are frequent causes of diarrhea in humans worldwide originating in foods of animal origin mainly from meat. The aim of this study was to determine the prevalence of Campylobacter spp. in lamb at different stages of the slaughter line including: More
Campylobacter jejuni/coli are frequent causes of diarrhea in humans worldwide originating in foods of animal origin mainly from meat. The aim of this study was to determine the prevalence of Campylobacter spp. in lamb at different stages of the slaughter line including: after-skinning, after evisceration and the end of slaughter process. A total of 150 lamb samples (50 samples per each stage) were collected over a period of 16-month between January 2006 and May 2008, and were analyzed for the presence of Campylobacter spp. According to the results, Campylobacter spp. were isolated from 11.3% (17/150) of the carcasses from the three sampling stages. Among the isolates, 76.5% were identified as C. jejuni and 23.1% as C. coli. Campylobacter spp. were isolated from 5%, 8% and 4% of carcasses during the stages of after-skinning, after-evisceration and the end of slaughter process, respectively. Antibiotics susceptibility of 17 isolates were determined for ten different antibiotics using the disk diffusion assay. Results revealed that 58/8% of the isolates were resistant to ciprofloxacin, while 47/1% of the isolates to nalidixic acid, 41/2% to tetracycline, 29/4% to enrofloxacin, 23/5% to ampicillin, 5/9% to amoxicillin, and 5/9% top streptomycine. None of the isolates was resistant to erythromycin, chloramphenicol and gentamicine. This study emphasizes the application of a preventive system such as HACCP (Hazard Analysis of Critical Control Points) for the control of Campylobacter contamination in slaughterhouse.
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AbstractCampylobacter spp infectious are one of the most important foodborne diseases in many countries and causing of diarrhea in more contries. In this study, a total of 100 edible mushrum (Agaricus mushrooms) were purchase in order to detection and identification of More
AbstractCampylobacter spp infectious are one of the most important foodborne diseases in many countries and causing of diarrhea in more contries. In this study, a total of 100 edible mushrum (Agaricus mushrooms) were purchase in order to detection and identification of Campylobacter jejuni and Campylobacter coli in edible mushrum at 2014 year in stores and retail market of Shahrekord city, Central of Iran. All of the samples were cultured in enrichment and specific bacteriological media and then use for Polymerase Chaine Reaction (PCR) method for detection Campylobacter jejuni and Campylobacter coli. Out of 100 samples, 15 % were positive due to Campylobacter spp. From 15 samples positive, 13.3 % and 86.7 % were positive to Campylobacter jejuni and Campylobacter coli, respectively. Therefore the consumption of raw edible mushrum to Campylobacter jejuni and Campylobacter coli constitutes health hazard for human in this city.Keywords: Campylobacter spp., Edible Mushrum, Retail market, PCR, ShahreKord,Iran
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Campylobacter is one of the main potentials for causing diarrhea and bacterial gastroenteritis in humans worldwide. The aim of this study was to determine the prevalence of campylobacter in poultry carcasses during slaughtering process in Najaf abad-Isfahan slaughterhou More
Campylobacter is one of the main potentials for causing diarrhea and bacterial gastroenteritis in humans worldwide. The aim of this study was to determine the prevalence of campylobacter in poultry carcasses during slaughtering process in Najaf abad-Isfahan slaughterhouse. In summer 2014, 160 samples were collected from chicken carcasses from 5 chicken flock during 8 different stage of slaughtering and analyzed for detecting campylobacter spp. The results showed that 102 samples from 160 samples (%63.75) were positive for campylobacter. The prevalence of campylobacter in different stage were 19 of 20 (%95) in cloaca, 16 of 20 (%80) predefeathering, 12of 20 (%60) post defaethering, 12 of 20 (%60) post evisceration, 14 of 20 (%70) post chilling, 13 of 20 (%65) post packing, 10 of 20 (%50) post deboning and 6 of 20 (%30) post freezing. Also biochemical differentiation of the produced campylobacteria isolates showed that C.jejuni was frequently isolated (%90.2) than C.coli (%9.8).
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Introduction and purpose: Campylobacter species are one of the most important pathogens causing bacterial gastroenteritis, which are generally transmitted through food of animal origin. The present study was conducted with the aim of genetic classification of Campylobac More
Introduction and purpose: Campylobacter species are one of the most important pathogens causing bacterial gastroenteritis, which are generally transmitted through food of animal origin. The present study was conducted with the aim of genetic classification of Campylobacter isolated from raw milk of cows, sheep and goats in Chaharmahal and Bakhtiari province.
Methods: 43 isolates of Campylobacter isolated from raw milk of cows, sheep and goats in Chaharmahal and Bakhtiari provinces were selected and confirmed by ERIC-PCR method.
Results: The studied isolates revealed banding patterns ranging from 100 to 2000 open pairs, which were further classified into 5 main profiles with a similarity coefficient of simple matching at a similarity level above 80%. Except for 100% affinity which was observed in 1 case, other isolates had genetic affinity between 54% and 98%.
Conclusion: The placement of the studied isolates in several subgroups showed the acceptable discrimination power of the ERIC-PCR technique in Campylobacter genotyping and the presence of different sources of contamination of dairy products with this pathogen. ERIC-PCR method is a simple, fast and low-cost method to describe the genetic diversity of different Campylobacter strains, including Campylobacter jejuni and coli strains.
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Background and objective: Campylobacter spp. are important causative agent of gastric infection worldwide, and contaminated water and foods are the major transmission factors of this bacterium to human. The major purpose of this study was isolation, identification and c More
Background and objective: Campylobacter spp. are important causative agent of gastric infection worldwide, and contaminated water and foods are the major transmission factors of this bacterium to human. The major purpose of this study was isolation, identification and characterization of Campylobacter spp. and Arcobacter spp. from the samples obtained from Caspian Sea in the North of Iran. Materials and methods: 263 water samples were collected throughout four seasons. Campylobacter spp. and Arcobacter spp. were isolated using standard methods and were identified by Phenotyping tests. Finally, the identification of these strains was verified by PCR method. Result: Foloowing phenotyping tests and their confirmation with molecular technique, totally seven Campylobacter jejuni strains and 14 Arcobacter butzelri strains were identified. Based on the results, the prevalence of this bacterium in the coastal waters of the Caspian Sea were evaluated as 2.66 and 5.32 percent. Conclusion: It is the first time that Campylobacter jejuni and Arcobacter butzelri were isolated from Caspian Sea. The epidemiologic studies regarding to the ways of their entrance in an environment and their maintenance in the habitat assist activists to control the water qualification and prevention from distribution of infections.
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گونههای کمپیلوباکتر شایعترین عامل اسهال باکتریایی در انسان میباشند. در تحقیق حاضر شیوع و مقاومت آنتیبیوتیکی گونههای کمپیلوباکتر جدا شده از شیر خام شهرستان ارومیه بررسیشدند. 80 نمونه شیر خام گاو بهصورت تصادفی و با شرایط سترون از خرده فروشیهای لبنیات سنتی مناطق م More
گونههای کمپیلوباکتر شایعترین عامل اسهال باکتریایی در انسان میباشند. در تحقیق حاضر شیوع و مقاومت آنتیبیوتیکی گونههای کمپیلوباکتر جدا شده از شیر خام شهرستان ارومیه بررسیشدند. 80 نمونه شیر خام گاو بهصورت تصادفی و با شرایط سترون از خرده فروشیهای لبنیات سنتی مناطق مختلف ارومیه در سال 1397 جمعآوری شدند. نمونهها، ابتدا در آبگوشت پرستون تکمیلشده، غنیسازی و سپس در آگار تکمیل شده کمپیلوباکتر کشت و در دمای 42 درجهسلسیوس بهمدت 48 ساعت در شرایط میکروآیروفیل گرمخانهگذاری شدند. آزمایشهای بیوشیمیایی ازجمله هیدرولیز هیپورات و حساسیت یا مقاومت به نالیدیکسیک اسید برای شناسایی گونهی جدایهها انجام شدند. آزمایش حساسیت آنتیبیوتیکی روی جدایهها بهروش انتشار دیسک کربی-بائر انجام شد. 13 نمونه (25/16 درصد) آلوده به گونههای کمپیلوباکتر بودند. شیوع جدایههای کمپیلوباکتر ججونای (75/13 درصد) بالاتر از کمپیلوباکتر کولای (5/2 درصد) بود. جدایههای کمپیلوباکتر مقاومت بالا در برابر تتراسایکلین (100 درصد)، کوتریموکسازول (84 درصد)، آمپیسیلین، سفتریاکسون و کلرامفنیکل (2/69 درصد) نشان دادند درحالیکه آنها مقاومت متوسط به سیپروفلوکساسین و نیتروفورانتوئین (2/46 درصد) و مقاومت پایین به جنتامایسین (8/30 درصد) نشان دادند. همچنین 9 جدایه (2/69 درصد) مقاومت چند دارویی (MDR) نشان دادند. میتوان نتیجهگیری نمود که شیوع گونههای کمپیلوباکتر و سویههای MDR آنها در شیر خام گاو توزیعی ارومیه بالا میباشد. ارتقا سلامت دام و بهداشت شیر، جلوگیری از مصرف بیش از حد آنتیبیوتیکها در مزارع گاوان شیری و پاستوریزاسیون شیر پیشنهاد میگردد.کلمات کلیدی: شیرخام، گونههای کمپیلوباکتر، الگوی مقاومت آنتی بیوتیکی، ارومیه، ایران
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