Using of ERIC-PCR, RAPD-PCR, Rep-PCR methods for genotyping of Campylobacter jejuni and Campylobacter coli strains isolated from raw chicken meat
Subject Areas : Food Microbial Contamination
Maryam Hadiyan
1
(Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran)
Hassan Momtaz
2
(Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran)
Amir Shakerian
3
(دانشگاه آزاد اسلامی واحد شهرکرد)
Keywords: Campylobacter jejuni, Campylobacter coli, ERIC-PCR, RAPD-PCR, Rep-PCR,
Abstract :
Background and aim: Campylobacter, especially the two species Campylobacter jejuni and Campylobacter coli, having different strains and hosts, are considered one of the most important and common pathogenic bacteria between humans and animals. The present study was conducted with the aim of genetic classification of Campylobacter jejuni and Campylobacter coli strains isolated from raw chicken meat. Materials and Methods: This cross-sectional study was conducted on 36 strains of Campylobacter jejuni and Campylobacter coli strains isolated from raw chicken meat. In order to genotyping the isolates, three methods ERIC-PCR, RAPD-PCR, and Rep-PCR were used. Results: 36 Campylobacter strains isolated from raw chicken meat were placed in 7 profiles in ERIC-PCR method, in 3 profiles in RAPD-PCR method and in 3 profiles in REP-PCR method. Conclusion: All investigated strains had a polymorphic band pattern, which shows a high rate of polymorphism in Campylobacter jejuni and Campylobacter coli genomes. The methods used in this study are powerful tools for genotyping of Campylobacter jejuni and Campylobacter coli, but according to the findings, the ERIC-PCR method is a more suitable method than other methods for typing and classifying Campylobacter jejuni and Campylobacter coli strains.
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