An assay for detection the frequency of Campylobacter and Arcobacters from Caspian Sea by bacterial culture and PCR techniques
Subject Areas : Medical Microbiology
Fahimeh Ghorbani Moein
1
(
Young Researchers Club, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran.
)
Masood Ghane
2
(
Department of Microbiology, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran
)
Keywords: Isolation, Caspian Sea, PCR, Campylobacter, Arcobacter,
Abstract :
Background and objective: Campylobacter spp. are important causative agent of gastric infection worldwide, and contaminated water and foods are the major transmission factors of this bacterium to human. The major purpose of this study was isolation, identification and characterization of Campylobacter spp. and Arcobacter spp. from the samples obtained from Caspian Sea in the North of Iran. Materials and methods: 263 water samples were collected throughout four seasons. Campylobacter spp. and Arcobacter spp. were isolated using standard methods and were identified by Phenotyping tests. Finally, the identification of these strains was verified by PCR method. Result: Foloowing phenotyping tests and their confirmation with molecular technique, totally seven Campylobacter jejuni strains and 14 Arcobacter butzelri strains were identified. Based on the results, the prevalence of this bacterium in the coastal waters of the Caspian Sea were evaluated as 2.66 and 5.32 percent. Conclusion: It is the first time that Campylobacter jejuni and Arcobacter butzelri were isolated from Caspian Sea. The epidemiologic studies regarding to the ways of their entrance in an environment and their maintenance in the habitat assist activists to control the water qualification and prevention from distribution of infections.
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