Molecular identification of Campylobacter species and assessment of the pathogenicity potential of these isolates in cattle, based on the presence of CDT cytotoxin genes
Subject Areas : Molecular Microbiologyommolbanin ghasemian 1 , Hamid Mahmoodipour 2
1 - Assistant Professor, Department of Veterinary, Behbahan Branch, Islamic Azad University, Behbahan, Iran.
2 - Assistant Professor, Department of Nursing and Midwifery, Behbahan Branch, Islamic Azad University, Behbahan, Iran.
Keywords: Cytolethal distending toxin, Campylobacter jejuni, Campylobacter coli, Cattle.,
Abstract :
Background & Objectives: Many Gram-negative bacteria have the ability to produce cell swelling toxins, which are composed of three subunits encoded by the cdtA, cdtB, and cdtC genes. The aim of this study was to determine the prevalence of cdtA, cdtB, and cdtC in Campylobacter jejuni and Campylobacter coli isolated from cattle. Materials & Methods: In this cross-sectional study, 152 stool samples were collected from cows. All samples were tested for the isolation of Campylobacter species using the prêt KB method. Then, suspected Campylobacter samples were authenticated using DNA sequencing method of 16SrRNA genes. Finally, Campylobacter isolates were evaluated for detection of cdtA, cdtB and cdtC genes. Results: Of the 152 samples, 14 (9.20%) of cow feces were positive for Campylobacter spp. Of these, nine samples (64.30%) were diagnosed with Campylobacter jejuni and five samples (35.70%) were diagnosed with Campylobacter coli. The prevalence of the cdtA, cdtB, and cdtC virulence genes in cattle was 64.29, 85.71, and 85.71%, respectively. These data show that cdtA, cdtB, and cdtC are widespread among Campylobacter isolates. Statistical analysis showed that there was no significant relationship between the occurrence of cdtA, cdtB, and cdtC and Campylobacter species with the host type. Conclusion: Resuls shows that most of these isolates produced CDT toxin at a high level and quantity. Therefore, Campylobacter strains isolated from cattle can be considered infectious agents for humans.
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