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Open Access Article
1 - تاثیر تیمارهای مختلف بر زندهمانی بذر و رویش نهالهای رملیک ()
محمدرضا حسنوند اسداله متاجی رضا اخوان -
Open Access Article
2 - Evaluation of the Physicochemical, Rheological, Sensory and Survival Characteristics of Bacteria in Functional Milk Dessert Containing Aloe Vera Gel
shamim barandeh marjaneh sedaghatiIntroduction: This research was carried out with the aim of investigating the possibility of producing functional milk dessert containing aloe vera gel with acceptable physicochemical, rheological and sensory properties. Materials and methods: Aloe vera gel was used at MoreIntroduction: This research was carried out with the aim of investigating the possibility of producing functional milk dessert containing aloe vera gel with acceptable physicochemical, rheological and sensory properties. Materials and methods: Aloe vera gel was used at four levels of 0, 3%, 6% and 9% for the production of milk dessert, and its physicochemical properties (pH, syneresis and viscosity), rheological properties (dynamic oscillation test), the sensory and survival characteristics of Lactobacillus plantarum bacteria during 20 days of storage were investigated. Results The results showed that the addition of aloe vera gel decreased the pH of milk dessert samples. The maximum stability of milk dessert samples was observed in sample T3 which contained 9% aloe vera gel. The results showed that in all samples, the viscosity increased significantly with increasing aloe vera gel concentration (p<0.05%). The addition of aloe vera gel significantly increased the survival rate of Lactobacillus plantarum in the treatment samples (p<0.05%). In the evaluation of the rheological properties of the milk dessert, it was found that the storage modulus (Gꞌ), viscous modulus (Gꞌꞌ) increased and the complex viscosity (ƞ*) decreased with the increase in aloe vera gel concentration. In the sensory evaluation, it was found that, the highest overall acceptance score belong to the T2 treatment. Conclusion: According to the results, using 6% aloe vera gel in milk dessert reduces the syneresis, increases the viscosity, improves the survival of Lactobacillus plantarum bacteria, and in terms of the sensory properties, it will increase the acceptability among the consumers. Manuscript profile -
Open Access Article
3 - The Effect of Microencapsulation on Survival of lactobacillus acidophilus and on the Quality Characteristics of Apple Juice During Storage at Ambient Temperature
shohreh sheikh ghasemi shahin zomorodiIntroduction: Probiotic apple juice is one of the newest product in healthy drink trade. The aim of this study is to investigate the survival of Lactobacillus acidophilus (LAFTI-L10 DSL) in both free and microencapsulated forms and also the effect of microencapsulation MoreIntroduction: Probiotic apple juice is one of the newest product in healthy drink trade. The aim of this study is to investigate the survival of Lactobacillus acidophilus (LAFTI-L10 DSL) in both free and microencapsulated forms and also the effect of microencapsulation of the the physicochemical and sensory properties of apple juice during 60 days of storage at 25± 5°C.Materials and Methods: This study consisted of four treatments: Control, apple juice containing Lactobacillus acidophilus in free form and encapsulated form and apple juice where the pH was regulated by the addition of whey protein to 4.4 with Lactobacillus acidophilus.Results: The results showed that the number of probiotics in apple juice containing Lactobacillus acidophilus in free and encapsulated form were reduced by two and one log cycle respectively and in apple juice where the pH was adjusted to 4.4 by whey protein using Lactobacillus acidophilus was increased by 1.2 log cycle during storage. The treatment concerned with whey powder showed the lowest transparency and color, while the encapsulated treatments showed the highest transparency and color and the lowest turbidity.Sensory properties of the samples containing free and encapsulated probiotic did not have significant differences as compared to the control Conclusion: It was concluded that the encapsulation might be used in the production of probiotic apple juice using Lactobacillus acidophilus. Manuscript profile -
Open Access Article
4 - The effect of Delijan reclaimed wastewater and well water on soil carbon sequestration and growth of hard wood trees
Zia Azdoo fatemeh Ahmadloo gholamreza Goodarzi Ali Farmahini Farahani Hashem KeneshloBackground & Objective: The reclaimed wastewater can be used as tool for compensation of water scarcity and control and reduce harvesting from deep and semi deep wells. Investigation on the effect of irrigation with Delijan reclaimed wastewater and well water for 5 MoreBackground & Objective: The reclaimed wastewater can be used as tool for compensation of water scarcity and control and reduce harvesting from deep and semi deep wells. Investigation on the effect of irrigation with Delijan reclaimed wastewater and well water for 5 years on soil carbon sequestration and growth of 7 hard woods trees species was the purpose of this study.Method: In the present study, seedlings were cultivated after ground preparing as a group at 3 × 3 m. spacing based on a randomized complete block design with three replications in March 2011. For determination of the effect irrigation type on soil carbon sequestration, in the fifth year, soil sampling was taken by drilling at soil five profiles from tree consecutive depths (0-30, 30-60 and 60-90 cm) under the crown of the trees. In evaluating the growth of hardwood tree species at the end of the fifth year, characteristics of height, collar diameter, canopy diameter, canopy cover area, percentage of survival and percentage of vitality were measured.Findings: The results of the study on the amount of soil carbon sequestration in different depths showed that irrigation with reclaimed wastewater increased the organic carbon content of the soil, which it’s the highest content is in the depth of 60-90 cm of soil. Among the species planted, in irrigation with well water, the highest height was in species of Ailanthus altissima and collar diameter, canopy diameter, canopy cover area, and percentage of vitality were in species of Melia azedarach. In irrigation with reclaimed wastewater, there were the highest height in species of Robinia pseudoacacia, diameter of collar in species of Elaeagnus angustifolia, canopy cover diameter and canopy cover area in species of M. azedarach and vitality in two species of M. azedarach and Celtis australis. There were the lowest percentage of survival in species of Acer negundo in both of irrigation system.Discussion and Conclusions: The main effect of irrigation type did not show significant statistical difference. Species of A. negundo was more susceptible to water supply and soil conditions and was not suitable for planting in this area. Species of M. azedarach is the most consistent in the present study. Manuscript profile -
Open Access Article
5 - اثرات ضد سرطانی عصاره هیدروالکلی گل انگشتانه(Digitalis nevrosa ) بر رده سلولی سرطان پستان MCF-7
زهرا کشتمند نسترن اصغری مقدم فاطمه جوادی -
Open Access Article
6 - Investigating the essential oil of the medicinal plant Thymus (Thymus vulgaris L.) in the production of probiotic dairy products by determining the viability of bacteria
farah farahani Alireza Tammimi Mohammad Reza Khatami nejadPurpose: The purpose of the present study was to investigate the essential oil of the medicinal plant Thymus vulgaris L. in the production of probiotic dairy products by determining the viability. Materials and methods: Thyme essential oil is used as a flavoring agent MorePurpose: The purpose of the present study was to investigate the essential oil of the medicinal plant Thymus vulgaris L. in the production of probiotic dairy products by determining the viability. Materials and methods: Thyme essential oil is used as a flavoring agent in the production of traditional and industrial dairy products (yogurt, buttermilk, and curd). To produce probiotic products and pasteurized dairy products, bacteria are inoculated and different concentrations of thyme essential oil (0, 25, 40, 70, 100 and 130 micrograms/ liter) are added to dairy products. The viability of bacteria in dairy products is checked at specific time intervals (0, 4, 8, 12 and 20 days). Findings: By increasing the concentration of thyme in traditional and industrial dairy products, there is a significant decrease in the logarithm of Lactobacillus and Bifidobacterium bacteria (P<0.05). The highest viability of bacteria was observed with low concentrations of thyme in traditional yogurt, and with moderate amounts of essential oil in buttermilk and traditional curd, within 20 days. The highest viability of bacteria is in yogurt and industrial butter with high concentration and in industrial curd with a decrease in the amount of essential oil. The highest viability of Bifidobacterium bacteria is found with high concentrations of thyme in yogurt, buttermilk and traditional curd (P<0.05), also with low concentrations of thyme in industrial yogurt and buttermilk, and with high concentrations in industrial curd. Conclusion: In industrial dairy products with thyme, the viability of Bifidobacterium bacteria is longer, and in traditional dairy products, the shelf life of Lactobacillus bacteria is longer. Manuscript profile -
Open Access Article
7 - Evaluation of Cultivation and Establishment Possibility of the Species of Forage Lupine in Tabriz Plain
Hassan Monirifar bahman Pasban EslamForage crops are considered as important components of sustainable farming systems. Legumes have a specific role in farming systems. In order to assess the possibility of growing lupine in Tabriz plain, an experiment based on randomized complete block design with three MoreForage crops are considered as important components of sustainable farming systems. Legumes have a specific role in farming systems. In order to assess the possibility of growing lupine in Tabriz plain, an experiment based on randomized complete block design with three replications was carried out at the East Azarbaijan Agriculture and Natural Research and Education Center, Iran. Three forage lupins varieties namely Dieta (Lupinus albus), Iris (L. angustifolius) and Pootallong (L. luteus) in this study were evaluated. The results indicated that establishment and survival percentage of varieties under farm condition were significantly different. The intraction effect of variety× days after planting on percentage of establishment among lupine speaces was significantly different. Seedling of Dieta and Iris varieties emerged 6 days after planting, but that of Pootallong could emergent after 9 days. Seedling of Iris and Pootallong varieties dried 27 and 18 days after planting, respectively. The trends of growth and establishment of Dieta variety in the farm was different from the other two varieties. It could achieve the peak emergence after 24 days. Although, Dieta lupine variety, by having 50.1% emergence in the field, was able to grow in the farm and produce seed, but it couldn't express acceptable seed yield and its component. Since the salinity of soil and water used in this experiment were 4580 and 5634 µs/m respectively, growth of these species, specially L. angustifolius and L. luteus (Iris and Pootallong varieties) came across sevrer limitations. More studies are needed to evaluate adaptability and planting time of Dieta cultivar in the region . Manuscript profile -
Open Access Article
8 - Effect of planting density on establishment and growth of Jatropha in Baluchestan, Iran
Mohammad Yosef Achak Hashem Keneshlo Hadi DarroudiIn recent years, declining of fossil fuel reserves, increasing greenhouse gas emissions and climate change effects have forced the global communities to reduce their dependence on fossil fuels, increasing attention to biofuels as a renewable energy source. The aim of th MoreIn recent years, declining of fossil fuel reserves, increasing greenhouse gas emissions and climate change effects have forced the global communities to reduce their dependence on fossil fuels, increasing attention to biofuels as a renewable energy source. The aim of this study was to investigate the effects of plant density on Jatropha seedlings growth in Balochestan climate (Bahokalat region in Chabahar). Jatropha adaptation was examined using a statistical design of randomized complete blocks at four planting densities of 1111, 1333, 2500 and 3333 plant per hectare (planting distances were 3×3, 3×2.5, 2×2 and 2×1.5 meters, respectively). Studied factors included survival percentage, total height, vigourity, collar and crown diameter. The results of one-way analysis of variance and Kruskal-Wallis test showed that the first year-study the vigourity of the seedlings was affected by planting distance, and the treatment with a density of 1111 plant per hectare had the best vigourity. But in the second year, survival percentage was affected by planting distance and there were no significantly difference in other parameters. In general, according to the results, it can be claimed in the first years, due to the small size of trees, intra-species competition is not much intense and the planting distance does not have much effect on the growth of planted seedlings. According to the more survival in wide planting distance, and predicting trees growth in the following years, it seems that the 3×3 planting distance is more practical and suitable. Manuscript profile -
Open Access Article
9 - Evaluation of increasing the production through selection of appropriate species of range and the influence of different planting methods on the amount of their production (case study: Shoorab ranges of Kashan)
morteza abtahiThe aimes of this research are increase rangeland production of desertes of central Iran and modification of the prevailing conditions fragile wilderness areas through proper identifying the species and planting them in came into force. In this study, three species: Se MoreThe aimes of this research are increase rangeland production of desertes of central Iran and modification of the prevailing conditions fragile wilderness areas through proper identifying the species and planting them in came into force. In this study, three species: Seidlitzia rosmarinus, Nitraria schoberi and Zygophyllum eichwaldii by planting potted seedlings treated without operation rippering (breaking the hard layer) was conducted in a split plot based on randomized complete block design. Rainfed culture was conducted in March and only in the first year of planting and irrigation was 3 times per year. Traits within 3 years of the project, including survival, height growth, vegetation canopy and production. Statistical analysis of the data showed that the effect of ripper stay survival, height growth on Seidlitzia rosmarinus meaningless and maximum vegetation canopy diameter (121.7cm) and production (978 gr) were treated without treatment. Nitraria schoberi was not affected by the plant survival ripper, but the highest growth in height (63.7 cm), vegetation canopy diameter (100 cm) and production (1413.3 gr) was treated ripper. Ripper treatment on Zygophyllum eichwaldii consists of all the characters and most survival (86%), high (63.4 cm), vegetation canopy diameter (73.4 cm) and production (287 gr) treated with ripper has been made. Therefore, Manysurvival and vegetation canopy rate, priority of culture in the region Seidlitzia rosmarinus, Nitraria schoberi and Zygophyllum eichwaldii, respectively. The ripper do not effected on growth and survival of Seidlitzia rosmarinus but effected on survival, height growth, canopy and the production of the Nitraria schoberi and Zygophyllum eichwaldii species. Manuscript profile -
Open Access Article
10 - Evaluation of irrigation levels and application of superabsorbent polymers on ecophysiological characteristics of rangeland plant Capper (Capparis spinosa L.)
Amin allah Bagherifard Yousef Hamidoghli Mohammadhasan Biglouei Mehrorang GhaediTo evaluate the effects of using various levels of superabsorbent and irrigation regimes on yields of Capparis spinosa plant an experiment was performed based on a randomized complete block design with three replications in the Research Field of Emamzadeh Jaafar Gachsar MoreTo evaluate the effects of using various levels of superabsorbent and irrigation regimes on yields of Capparis spinosa plant an experiment was performed based on a randomized complete block design with three replications in the Research Field of Emamzadeh Jaafar Gachsaran in 2015. The main factor consisted of three irrigation regimes (once in a month, once every two months, and no irrigation) and a sub-factor at four levels of superabsorbent by Tarawat A200 applications (no superabsorbent, 75, 150, and 225 g/plant). Results revealed that the highest number of branches, collar diameter, height, and chlorophyll belonged to the plants irrigated every two months with 150 g superabsorbent and the highest value of carotenoids was observed in control plants. The highest plant survival rate was observed in 225 g/plant superabsorbent treatment alone. Irrigation levels had positive effects on the morphological characteristics of the plant so that the irrigation level of once every two months had more yield than the other levels. Findings suggest that the application of superabsorbent material in dry land condition could increase the yield by mitigating the effects of the drought stress. Manuscript profile -
Open Access Article
11 - ارزیابی عملکرد ریزپوشانی به همراه عصارههای گیاهی بر زنده مانی باکتریهای پروبیوتیک در طی مدت نگهداری در آب میوه
وحید کوشکی آرش بابایی معصومه مهربان سنگ آتش امید صفری -
Open Access Article
12 - The Study Effect of Spirulina platensis on Physicochemical, Microbial and Sensory Properties of Probiotic Dough Containing Lepidium sativum Seed Gum
Pegah Jalalvand Alireza Shahab Lavasani Orang Eyvaz Zadeh -
Open Access Article
13 - Quantitative and qualitative assessment of cells in several commercial probiotic products and evaluation of storage conditions on cell culturability and viability of a probiotic yeast
Fatemeh Hakimipour Rasoul Shafiei Mohammad Rabbani khorasganiOne of the essential criteria for determining the quality of products containing probiotics is the presence of a certain number of viable cells per gram of product. To transmit enough viable cells to the gastrointestinal tract, the population of probiotic microorganisms MoreOne of the essential criteria for determining the quality of products containing probiotics is the presence of a certain number of viable cells per gram of product. To transmit enough viable cells to the gastrointestinal tract, the population of probiotic microorganisms should be preserved during storage. In the present study, culturable cells of some commercial products were isolated and enumerated by the plate count and flow cytometric methods. The isolates were then identified by biochemical and molecular methods. The viability of yeast cells was then assessed at different temperatures during 30 days by the methods above. The results showed that Protexin restore® contained the same types and number of bacteria that were claimed by the manufacturer. In contrast, protexin balance® and Dailyeast® contained a lower number of bacterial cells and yeast cells, respectively. It is noteworthy that only 33% of yeast cells were culturable on YPG agar, while flow cytometric analysis showed that 87% of cells were viable and exhibited cell membrane integrity. Also, yeast cells in Dailyeast® product were stable at 4-35°C. In conclusion, using different culture media and physicochemical conditions for the enumeration of viable and culturable cells may lead to different results. Thus, a combination of different methods should be used to get good results. Manuscript profile -
Open Access Article
14 - The study of the production of mixture of Carrot juice and ice cream containing Bifidobacterium lactis and Lactobacillus casei
hanieh ghandali Alireza Shahab Lavasani sara movahhedThis study was performed to produce a probiotic carrot juice-ice cream beverage. Designed in a random factorial CRD manner. For this purpose, both Lactobacillus casei and Bifidobacterium lactis (at 108 and 109 cfu/ml levels) were added to beverage formulation. The sampl MoreThis study was performed to produce a probiotic carrot juice-ice cream beverage. Designed in a random factorial CRD manner. For this purpose, both Lactobacillus casei and Bifidobacterium lactis (at 108 and 109 cfu/ml levels) were added to beverage formulation. The samples were stored at -5ºC for 21 days. Survival of bacteria, pH, titrable acidity (%), beta-carotene (%), total solids contents (%), phenolic components (µg/ml) and DPPH (%) were measured at 1, 6, 11, 16 days of storage. The number of viable cells were significantly decreased during the storage time. However, the number of L. casei and Bf. lactis were in the recommended range (106 cfu/ml) until last day of storage. The highest number of viable cells was for Bf. lactis. By increment the probiotics levels, the pH and total solids values were decreased. Treatments and storage time did not show any significant effect on the acidity of probiotic carrot juice-ice cream. The highest amounts of phenolic components were for 109 cfu/ml L. casei and 108 Bf. Lactis. During storage period, the phenolic compounds and antioxidant activity of all samples were significantly decreased. Increment the concentration of L. casei in beverage formulation leads to decreased DPPH percentage inhibitory, while, by increasing the Bf. lactis in samples, the antiradical activity was increased. Samples containing 109 cfu/ml L. casei and Bf. Lactis had the highest amounts of beta-carotene. According to obtained results, treatment E which contained 109 cfu/ml bifidobacteriun lactis was the best among other treatments. Manuscript profile -
Open Access Article
15 - مطالعه اثرات دوره سرمایی بر کیفیت اسپرم بزهای سانن و جاموناپاری
تی. اسواراموهان کا. نیلانی پی. سورکا پی. ماهادوان ک. بالاسوبرامانیامهدف از مطالعه حاضر آزمودن اثرات دوره سرمایی بر کیفیت منی بزهای سانن و جاموناپاری بود. منی نژادهای بز با استفاده از واژن مصنوعی جمعآوری و در دمای 5 تا 9 درجه سانتیگراد سرد شد. حجم، رنگ و pH منی تازه تعیین شد. پس از رقیقسازی، آزمایش میکروسکپی از نظر تحرک پیشرونده و زند Moreهدف از مطالعه حاضر آزمودن اثرات دوره سرمایی بر کیفیت منی بزهای سانن و جاموناپاری بود. منی نژادهای بز با استفاده از واژن مصنوعی جمعآوری و در دمای 5 تا 9 درجه سانتیگراد سرد شد. حجم، رنگ و pH منی تازه تعیین شد. پس از رقیقسازی، آزمایش میکروسکپی از نظر تحرک پیشرونده و زندهمانی اسپرم صورت گرفت. منی رقیق شده دو نژاد به وسیله آزمایش میکروسکپی از روز 0 تا 3 سرمادهی مورد مقایسه قرار گرفت. هموسایتومتر جهت ارزیابی شمار اسپرمها با استفاده از 10 میکرولیتر منی بارگذاری شد. از رنگآمیزی ائوزین (1 درصد) برای تخمین اسپرمهای زنده استفاده شد. گرچه زندهمانی و سرعت حرکت اسپرمهای دو نژاد بز به طور وابسته به زمان در طی سرمادهی کاهش یافت (05/0>P)، زندهمانی بین بزهای سانن و جاموناپاری به طور معنیدار تفاوت نداشت. در هرحال، تفاوت معنیداری برای میانگینهای سرعت حرکت اسپرم مشاهده شد (05/0>P). تحرک پیشرونده و زندهمانی به طور تدریجی در طی سرمادهی در 5 تا 9 درجه سانتیگراد کاهش داشت. به منظور کسب نرخ موفق بالاتر تلقیح، باید منی سرمادهی شده بز برای دامداران در طی یک روز فراهم شود. Manuscript profile -
Open Access Article
16 - تحرک، زندهمانی و نابهنجاری اسپرماتوزوآ گاو نر Bali با رقیق کنندههای Andromed و تریس-زرده تخم مرغ نگهداری شده در دمای 4 درجه سلسیوس
ر. سوهاردی م. مگاواتی ف. اردهانی پ. سامپون س. ووتیسوتیمتوگاو نر Bali پتانسیل بالایی برای توسعه به­ عنوان یک منبع گوشت ملی در اندونزی دارد که میتواند نگرانیهای مربوط به واردات گوشت را برطرف کند. پرورش گاو نر Bali تنها نیازمند سیستمهای نگهداری کوچک است، دارای باروری بالا و مرگ­و­میر گوساله پایین به ­عنوان مزا Moreگاو نر Bali پتانسیل بالایی برای توسعه به­ عنوان یک منبع گوشت ملی در اندونزی دارد که میتواند نگرانیهای مربوط به واردات گوشت را برطرف کند. پرورش گاو نر Bali تنها نیازمند سیستمهای نگهداری کوچک است، دارای باروری بالا و مرگ­و­میر گوساله پایین به ­عنوان مزایای آن یک سرمایهگذاری در حال رشد از طریق تلقیح مصنوعی (AI) با منی جمعآوری شده گاوهای نر برتر فنوتیپی است. با این حال، ابزار کارآمد برای حفظ کیفیت منی گاو نر Bali با استفاده از رقیق­کنندهها هنوز هم نیاز به اکتشاف در سطح ملی دارد. هدف از این پژوهش تعیین اینکه کدام یک از رقیق­کنندههای انتخاب شده به بهترین وجه کیفیت مایع منی گاو نر Bali محلی را برای مدت پنج روز در دمای 4 درجه سلسیوس حفظ میکند. رقیق­کنندههای Andromed و تریس-زرده تخم مرغ در حفظ کیفیت منی جمع آوری شده از گاوهای هفت ساله با حداقل تحرک 70 درصد مقایسه شدند. تحرک، زندهمانی، و نابهنجاری اسپرماتوزوآ مشاهده و مقایسه شدند. این تحقیق از طرح فاکتوریل کاملا تصادفی با دو فاکتور که هر فاکتور دارای دو سطح و پنج سطح با سه سطح زمانی استفاده کرده است. نتایج این مطالعه نشان داد اسپرماتوزوآ گاو نر Bali 51.66 درصد تحرک، 59.13 درصد زندهمانی، و تنها 22.68 درصد نابهنجاری تا روز پنج در رقیق­کننده تریس-زرده تخم مرغ نشان داده است. در مقایسه، رقیق­کننده Andromed 33/23 درصد تحرک، 59/45 درصد زندهمانی، و 23.15 درصد نابهنجاری تحت شرایط مشابه نشان داده است. برای نتیجه­گیری، تریس-زرده تخم مرغ یک رقیق کننده قوی هنگام مقایسه با Andromed در نگهداری کیفیت اسپرم گاوهای بومی Bali با بهترین حفظ تحرک، زندهمانی اسپرماتوزوآ و کمترین درصد نابهنجاری هنگام ذخیره برای 5 روز در دمای 4 درجه سلسیوس است. این دادهها اطلاعات مفیدی برای پرورش دهندگان گاو نر فراهم میکند، به ویژه پرورش دهندگان گاو نر Bali در اندونزی در عمل تلقیح مصنوعی برای در نظر گرفتن دقیق انتخاب رقیقکننده نسبت به زمان نگهداری و دما در بهبود روشهای پرورش گاو برای افزایش بهرهوری گوشت در این منطقه میباشد. Manuscript profile -
Open Access Article
17 - The Cytotoxic Effect of Nandrolone on Colon Cancer Cell Line and the Evaluation of the Caspase-3, 8 and 9 activity
Aahra Aalaei Abdolhossein Shiravi Rahim Ahmadi Vida HojatiThe studies indicated that sex steroid hormones affect the digestive system function and proliferation of the cancer cells in the cell and molecular level. This study examines the anti-proliferative effects of Nandrolone on colon cancer (HCT) cell line and evaluates the MoreThe studies indicated that sex steroid hormones affect the digestive system function and proliferation of the cancer cells in the cell and molecular level. This study examines the anti-proliferative effects of Nandrolone on colon cancer (HCT) cell line and evaluates the activity of caspases of 3, 8, and 9. In this experimental study, HCT cells were divided into the control group (without treatment) and groups treated with 0.625, 1.25, 2.5, 5, and 10 mg/ml of Nandrolone. The cytotoxic effect of hormone was measured using MTT. The activity level of caspases 3, 8, and 9 were evaluated by the standard kit. The data were compared among the groups using one-way analysis of variance and t-test. HCT cell viability significantly decreased in the groups treated with 2.5, 5, and 10 mg/ml of Nandrolone compared to the control group (p < 0.001); However, caspase 3 and 9 (p < 0.001) and caspase 8 activity (p < 0.01) significantly increased. This study's findings showed that Nandrolone has a cytotoxic effect on colon cancer cells, and this effect is partly mediated by increasing the caspases of 3, 8, and 9 activities. Manuscript profile -
Open Access Article
18 - The Study of the Viability of Human Wharton's Jelly Mesenchymal Stem Cells in Alginate Capsules
Zahra Poursafavi Saeed Abroun Saeid Kaviani Jebeli Nasim Hayati RoudbariStem cells with high proliferative capacity can be isolated from different tissues of the body. These cells originate from the fetal mesoderm and are found in tissues such as bone marrow, fat tissue, amniotic fluid, and Wharton's jelly. In this study, the survival of Wh MoreStem cells with high proliferative capacity can be isolated from different tissues of the body. These cells originate from the fetal mesoderm and are found in tissues such as bone marrow, fat tissue, amniotic fluid, and Wharton's jelly. In this study, the survival of Wharton's jelly human mesenchymal stem cells inside alginate capsules after 7, 14 and 21 days has been investigated. In this experimental study, 10 umbilical cord samples were obtained from pregnant mothers during caesarean section, and the vessels of the umbilical cord samples were isolated. Then it was cultured in DMEM-HG medium containing 10% FBS serum for 5 days. To show the stemness of these cells, CD73, CD34 and CD45 markers were evaluated by flow cytometry technique. After confirmation, the cells were encapsulated in alginate hydrogels. The viability of encapsulated cells was evaluated by trypan blue and MTT. The results showed that the capsules are spherical and have a uniform border and are homogeneously dispersed throughout the capsule. Wharton jelly encapsulation of mesenchymal stem cells did not change their morphology and viability. After 21 days, the survival of the encapsulated cells was maintained. Alginate as a three-dimensional biodegradable scaffold with suitable cell viability can be used as a suitable option for cell therapy and tissue engineering with the property of non-graft rejection. Manuscript profile
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