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      • Open Access Article

        1 - Quantitative Assessment of CK-18 Gene Expression in Patients Suffering from Chronic Myeloid Leukemia
        Farshid Ghaidar Nastaran Asghari Moghaddam fariba Sharifnia
        Chronic Myeloid Leukemia (CML) is one of the most common cancers. Its incidence has been increased during recent years and leads to high mortality if it is diagnosed late. Currently, most laboratory and diagnostic methods are difficult, cost- and time- consuming and thi More
        Chronic Myeloid Leukemia (CML) is one of the most common cancers. Its incidence has been increased during recent years and leads to high mortality if it is diagnosed late. Currently, most laboratory and diagnostic methods are difficult, cost- and time- consuming and this is the major obstacle to early detection of cancer. Cytokeratin 18 (CK18) is one of the cytoskeletal proteins which expression pattern change has been observed in various cancers. The present study investigates the altered expression of this gene at mRNA level in people who with recently diagnosed CML. In order to achieve this goal, blood samples were collected from 50 CML patients and 50 healthy individuals. Then, RNA extraction was performed and relative expression of CK-18 gene was measured by Real-time PCR. Data were analyzed by 2-ΔΔCT. Biomarker potential was evaluated by ROC curve. Results showed a 1.92-fold increase in the expression of CK-18 gene in leukemia samples compared to normal ones (p<0.05). No significant relationship was found between gender and changes in gene expression in case group. The results of this study elaborate that evaluation of CK-18 mRNA could be useful to detect CML along with other factors. Manuscript profile
      • Open Access Article

        2 - Taxonomic value of flower morphology and spur in Persian Delphinium
        Masoomeh Hasanbarani fariba Sharifnia Mostafa Assadi
        The genus Delphinium belongs to the Delphinieae tribe and Ranunculaceae family; in Iran according to flora Iranica has 29 species with zygomorphic flower. In this study, the morphological information of flower and spur in Delphinium species was studied to determine the More
        The genus Delphinium belongs to the Delphinieae tribe and Ranunculaceae family; in Iran according to flora Iranica has 29 species with zygomorphic flower. In this study, the morphological information of flower and spur in Delphinium species was studied to determine the importance of these characters in identifying species of this genus. Inflorescence condition, flower color, sepal flower, the color of sepals and petals comparative to each other, spur shape, spur curvature and spur tip were investigated. Statical analysis was conducted by SPSS software and cluster analysis was performed by Average linkage method. The results showed that flower color is one of the important traits in identifying Iranian Delphinium species, so that these genera are divided into two groups of yellow and non-yellow flower. Also, shape and spur curvature can differ from cylindrical, attenuate and inflatum. However, these character don’t correlate with distribution and the area where plant is grown. D. saniculifolium has a inflatum spur among specimens. Herbarium image of flower in different species are presented. Manuscript profile
      • Open Access Article

        3 - Pollen morphology of Delphinium in Iran
        Masoomeh Hasanbarani fariba Sharifnia Mostafa Assadi
        Delphinieae tribe in Ranunculoideae subfamily has about 700 species. Delphinium is one of the genus in Delphinieae that has 29 species and two subgenus in Iran. Differences between subgenera are in the form of seed and vegetative period. Olighophyllon and Delphinium, wh More
        Delphinieae tribe in Ranunculoideae subfamily has about 700 species. Delphinium is one of the genus in Delphinieae that has 29 species and two subgenus in Iran. Differences between subgenera are in the form of seed and vegetative period. Olighophyllon and Delphinium, which are perennial and annual species, respectively. In this study pollen grains of 27 species were examined by Scaning Electron Microscope. Pollen grains were grouped into spheroidal, prolate-spheroidal and subprolate-perprolate. In this genus surface ornaments of grain were seen as scabrate ،microperforate scabrate- and scabrate-reticulate. To investigate similarities among genus for palynology characteristics, cluster analysis was performed by SPSS. Also, PCA analysis was performed and the most variable characteristics were determined. The results showed that the first component included P / E ratio traits, polar axis length and colpi length size, and the second component included equatorial axis diameter and mesocolpium size traits, which played the most important role in the similarities and differences of taxons. Micro-morphological studies of Delphinium pollen in this study show that closely related species do not have very similar morphological characters. Pollen characters could not group the species studied. Pollen Phenogram in this study provides useful information to determine the status of D. elbursense, D. saniculifolium, D. aquilegifolium, and many other species. Manuscript profile