Pollen morphology of Delphinium in Iran

Subject Areas : biology

Masoomeh Hasanbarani ¹ , fariba Sharifnia ² , Mostafa Assadi ³

1 - مدرس حق التدریس
2 - دانشگاه آزاد اسلامی
3 - موسسه تحقیقات جنگل ها و مراتع کشور

Received: 2020-02-04 Accepted : 2020-05-21 Published : 2019-05-22

Keywords: Cluster analysis, PCA analysis, Delphinium, Scaning Electron Microscope,

Abstract :

Delphinieae tribe in Ranunculoideae subfamily has about 700 species. Delphinium is one of the genus in Delphinieae that has 29 species and two subgenus in Iran. Differences between subgenera are in the form of seed and vegetative period. Olighophyllon and Delphinium, which are perennial and annual species, respectively. In this study pollen grains of 27 species were examined by Scaning Electron Microscope. Pollen grains were grouped into spheroidal, prolate-spheroidal and subprolate-perprolate. In this genus surface ornaments of grain were seen as scabrate ،microperforate scabrate- and scabrate-reticulate. To investigate similarities among genus for palynology characteristics, cluster analysis was performed by SPSS. Also, PCA analysis was performed and the most variable characteristics were determined. The results showed that the first component included P / E ratio traits, polar axis length and colpi length size, and the second component included equatorial axis diameter and mesocolpium size traits, which played the most important role in the similarities and differences of taxons. Micro-morphological studies of Delphinium pollen in this study show that closely related species do not have very similar morphological characters. Pollen characters could not group the species studied. Pollen Phenogram in this study provides useful information to determine the status of D. elbursense, D. saniculifolium, D. aquilegifolium, and many other species.