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  1 - The inhibitory effect of water ozonation on reduction of Aspergillus parasiticus growth during wheat tempering
  sasan rezaie
  20.1001.1.17354226.1380.0.0.1.4
  Introduction and Objectives: Food and feed spoilage moulds cause great economic losses worldwide. Wheat is important cereal in Iran and very sensitive to mould contamination specially aflatoxigenic moulds. Ozone recognized as a strong disinfection substance since before More

  Introduction and Objectives: Food and feed spoilage moulds cause great economic losses worldwide. Wheat is important cereal in Iran and very sensitive to mould contamination specially aflatoxigenic moulds. Ozone recognized as a strong disinfection substance since before 1997. It decomposes rapidly to molecular oxygen without leaving a residue.Materials and Methods: In order to study the effect of ozonated water on growth of Aspergillus parasiticus, wheat samples inoculated with spore suspension (10, 102,104 spore/gr wheat) of Aspergillus parasiticus and incubated at 25-30◦Cfor 10 days. Contaminated samples incubated at <20, 25,40◦C for 24h,after tempering with ozonated water to concentrations of 0, 1, 2, 2.5 mg ozone/l. For evaluate of ozone effectiveness on reduction of Aspergillus parasiticus, weighing of mould mycelium method was used after drying.Then fungal mycelia were weighted to evaluate the effect of ozone on Aspergillus parasiticus growth. Result: Results indicated that ozonated water can reduce Aspergillus parasiticus growth and this the reduction can be increased due to increasing of ozone concentration. Tempering with ozonated water had significant effect on A. parasiticus growth (P< 0.001) . Conculusion: Therefore the ozonated water usage in wheat tempering can be caused reduction of Aspergillus parasiticus growth.Key words: Wheat, ozonation, tempering, Aspergillus parasiticus Manuscript profile
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  2 - The effect of dehydration stress on the expression of MYB, AP2, mir-172 and mir-159 genes in the leaves of Narcissus pseudonarcissus L.
  Maryamalsadat kamishirazi Ahmad Majd sedighe arbabian Golnaz Tajadod
  20.1001.1.17354226.1380.0.0.2.5
  Advances in the world are causing changes in the living conditions of living things, including plants. Plants must be able to adapt to environmental stressful conditions in a variety of ways, even molecular methods. One mechanism is to regulate gene expression after tra More

  Advances in the world are causing changes in the living conditions of living things, including plants. Plants must be able to adapt to environmental stressful conditions in a variety of ways, even molecular methods. One mechanism is to regulate gene expression after transcription by miRNAs (microRNAs). MicroRNAs often have 20 to 22 nucleotides, some of whose target genes belong to transcription factors. The expression of microRNAs changes in response to dehydration stress. In the present study, Narcissus bulbs were grown under different irrigation conditions (from once a week to once every two months). The leaves of 60-day-old plants were used to extract miR172-miR159 by q PCR method. The results showed a significant increase in the expression of MYB and AP2 genes and no expression of 159 and 172 microRNAs in the treated and control samples. Therefore, miR172-miR159 is not affected by dehydration stress. Given that the expression of dehydration genes has been evaluated for the first time in the Narcissus pseudonarcissus, therefore, this study can provide a good basis for further investigation in this case. Manuscript profile