The effect of dehydration stress on the expression of MYB, AP2, mir-172 and mir-159 genes in the leaves of Narcissus pseudonarcissus L.

Subject Areas : genomics

Maryamalsadat kamishirazi ¹ , Ahmad Majd ² , sedighe arbabian ³ , Golnaz Tajadod ⁴

1 - Army Highway, Mini City, Baharan, No. 1
2 - Department of biology- Faculty of biological science-Azad university-North Tehran Branch
3 - Department of Biology, Faculty of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran
4 - Department of Biology, Faculty of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran

Received: 2020-06-30 Accepted : 2020-08-29 Published : 2001-12-22

Keywords: Drought stress, miRNA159, miRNA172, MYB, AP2,

Abstract :

Advances in the world are causing changes in the living conditions of living things, including plants. Plants must be able to adapt to environmental stressful conditions in a variety of ways, even molecular methods. One mechanism is to regulate gene expression after transcription by miRNAs (microRNAs). MicroRNAs often have 20 to 22 nucleotides, some of whose target genes belong to transcription factors. The expression of microRNAs changes in response to dehydration stress. In the present study, Narcissus bulbs were grown under different irrigation conditions (from once a week to once every two months). The leaves of 60-day-old plants were used to extract miR172-miR159 by q PCR method. The results showed a significant increase in the expression of MYB and AP2 genes and no expression of 159 and 172 microRNAs in the treated and control samples. Therefore, miR172-miR159 is not affected by dehydration stress. Given that the expression of dehydration genes has been evaluated for the first time in the Narcissus pseudonarcissus, therefore, this study can provide a good basis for further investigation in this case.