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      • Open Access Article

        1 - Genetic variability of agronomic traits and biodiversity in the genus Ocimum
        Rajendra P. Patel Rakshapal Singh Raj Kishori Lal Pankhuri Gupta Anju Kesarwani Nitu Goyal
        The present study was performed to explore genetic and chemotypic variability among five Ocimum species. Genotypes were evaluated from pooled data over three locations for two consecutive years (2007-2009). High genotypic coefficient variation and phenotypic coefficient More
        The present study was performed to explore genetic and chemotypic variability among five Ocimum species. Genotypes were evaluated from pooled data over three locations for two consecutive years (2007-2009). High genotypic coefficient variation and phenotypic coefficient variation was observed for leaf area (63.55 and 63.80, respectevely), essential oil yield (40.37 and 41.0, respectevely) and leaf width (33.91 and 39.29, respectevely). Maximum heritability estimate in broad sence was observed for leaf area (99.20%) and leaf length (98.79%) and essential oil (98.36%). High genetic advance over mean was observed for the essential oil yield (80.59%), leaf width (74.43%), leaf length (69%) and essential oil content (64.71%). In addition, path coefficient analysis showed maximum positive direct effects of plant canopy (4.21), leaf width (3.8), followed by essential oil (2.49%) and fresh herb yield (0.95). The essential oils of Ocimum species were grouped in four chemical groups of compounds. The accessions of O. tenuiflorum, O. basilicum and O. gratissimum were found to be rich with phenylpropanoids.The results clearly showed variation in important economic traits can facilitate selection for further improvement inOcimum genotypes. Manuscript profile
      • Open Access Article

        2 - Quantification of ursolic acid, correlations and contribution by other traits towards accumulation of ursolic acid in six Ocimum species
        Raj Kishori Lal Smita Singh Pankhuri Gupta Sunita Dhawan Sougata Sarkar Ram Verma
        Sixteen accessions belonging to six species of Ocimum from Uttar Pradesh (12), Andhra Predesh (3) and Marashtra (1) states of India were screened for ursolic acid and higher oil yields. A considerable amount of genetic variability in morphometric traits was recorded in More
        Sixteen accessions belonging to six species of Ocimum from Uttar Pradesh (12), Andhra Predesh (3) and Marashtra (1) states of India were screened for ursolic acid and higher oil yields. A considerable amount of genetic variability in morphometric traits was recorded in all accessions. It was realized that both the estimate of heritability broad sense in percent (^h² %) and the corresponding genetic advance (GA) were high for oil yield (98.80 and 120.35) and herb yield/plant (93.52 and 89.27). However, high genetic heritability and moderate genetic advance expressed by days to flower 50% (95.13 and 49.07) followed by plant height (93.54 and 52.17) and ursolic acid yield (99.99 and 78.14), respectively. The ursolic acid expressed ^h² % and low GA (99.99 and 0.362) followed by oil content, leaf/stem ratio and ursolic acid content (99.99, 0.362). Correlation coefficients among the ten traits indicated that leaf/stem ratio was positively highly and significantly correlated with oil content (0.743**, 0.710**) and oil yield (0.700**, 0.676**). Leaf/stem ratio was also positively and significantly correlated with ursolic acid yield (0.551*, 0.536*). The herb yield was highly positive and significantly correlated with oil yield (0.790**, 0.772**). The oil content also had a high and significant correlation with oil yield (0.877**, 0.867**). In addition, similar to the aforementioned correlations, the ursolic acid was significantly and positively correlated with ursolic acid yield (0.966**, 0.965**) at both genotypic and phenotypic level. The path coefficient revealed that the highest direct contribution to ursolic acid was made by ursolic acid content (0.904) and herb yield (0.264). The oil content had the maximum positive indirect effect (0.471) to the ursolic acid content. The residual effect value was found to be 0.0248. Ocimum accessions CIM Ayu followed by Sel-1, CIM Jyoti, CIM Snigdha and CIM Shurabhi may be exploited for commercial cultivation. Manuscript profile