Droplet Counter Current Chromatography (DCCC) in herbal analysis
Subject Areas : Phytochemistry: Isolation, Purification, Characterization
Lutfun
Nahar
1
(Centre for Natural Products Discovery (CNPD), School of Pharmacy and Biomolecular Sciences, John Moores University, James Parsons Building, Byrom Street, Liverpool L3 3AF, United Kingdom|Laboratory of Growth Regulators, Institute of Experimental Botany ASCR & Palacký University, Šlechtitelů 27, 78371 Olomouc, Czech Republic)
Satyajit D.
Sarker
2
(Centre for Natural Products Discovery (CNPD), School of Pharmacy and Biomolecular Sciences, John Moores University, James Parsons Building, Byrom Street, Liverpool L3 3AF, United Kingdom)
Keywords: Cornus florida, Droplet Counter Current Chromatography (DCCC), Hedera helix berries, High-speed counter-current chromatography (HSCCC),
Abstract :
About half a century ago, the separation science was blessed with the introduction of a new liquid-liquid separation technique called droplet counter current chromatography (DCCC) that combined principles of counter current distribution and counter current chromatography, and employed a liquid stationary phase held in a cluster of vertical glass columns connected in series. In fact, DCCC is based on the partitioning of solutes between a constant stream of droplets of mobile phase and a column of surrounding stationary phase.
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