In vitro assessment of the antihyperglycemic property of Prosopis farcta J. F. Macbr and Rheum ribes L. and phytochemical profiling of the most active extract

Subject Areas : Phytochemistry: Isolation, Purification, Characterization

Ismael Hasan Mohammed ¹ , Vahed Zarial ² , Mahdi Moridi Farimani ^{3 *} , Maryam Kharatha ⁴ , Marzieh Tabefam ⁵ , Esmail Salih Ibrahim Kakey ⁶

1 - Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C., Evin, Tehran, Iran
2 - Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C., Evin, Tehran, Iran
3 - Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C., Evin, Tehran, Iran
4 - Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C., Evin, Tehran, Iran
5 - Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C., Evin, Tehran, Iran
6 - Department of Biology, Faculty of Health and Science, Koya University, Iraq

Received: 2025-02-22 Accepted : 2025-06-24 Published : 2025-06-25

Keywords: α-Glucosidase, Flavonoid, Phytochemical analysis, Prosopis farcta, Rheum ribes, Terpenoid,

Abstract :

This study aimed to evaluate the α-glucosidase inhibition potential of Prosopis farcta and Rheum ribes. For this purpose, various plant parts—including the root bark, roots, fruits, and leaves of P. farcta, as well as the roots and stalks of R. ribes—were sequentially extracted employing n-hexane, ethyl acetate, and methanol. The α-glucosidase inhibition potential of the extracts was assessed. Subsequently, the phytochemical investigation was carried out on the ethyl acetate extract derived from the root of P. farcta, which exhibited the most pronounced inhibitory effect. This analysis resulted in the isolation and identification of six compounds, including four steroids, one pentacyclic triterpenoid, and one flavonoid. The isolated compounds underwent further evaluation for their α-glucosidase inhibition potential. Among them, daucosterol (3) demonstrated the highest potency, with an IC50 value of 18.6 µM.

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