Veronica crista-galli Steven and Veronica persica Poir. as anticancer and antioxidant plants in-vitro
Subject Areas : Phytochemistry: Isolation, Purification, Characterization
Maryam Mohadjerani
1
,
Sedigheh Asadollahi
2
1 - Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran
2 - Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran
Keywords: Medicinal Plants, antioxidant activity, Anticarcinogen, <i>Veronica crista-galli</i>, <i>Veronica persica</i>,
Abstract :
The antioxidant activities and reducing power capabilities of Veronica crista-galli and Veronica persica were evaluated using DPPH radical scavenging assay, total antioxidant activity and MTT assay at incubation times of 24, 48 and 72 hours. A water fraction ofmethanolic extract of V. crista-galli with the highest amount of total flavonoid (16.19 ± 0.001 mg of QE/g of dried plant) demonstrated stronger antioxidant effect than V. persica extract with the highest content of phenolic compounds (60.75 ± 0.0013 mg GAE/g of dried plant). The IC50 values of V. persica and V. crista-galli extracts were found to be 153.7 and 79.64 μg/mL, respectively. V. crista-galli possessed considerable inhibitory effect at lower concentrations on MCF-7 and Hela cell lines by increasing incubation time. Therewas no cytotoxic effect observed in the normal HDF cell line. This study recommends that the water fraction of the methanolic extracts of V. persica and V. crista-galli with in vitro antioxidant and selective anti-proliferative activities can support the popular uses ofVeronica species in traditional medicine as effective natural remedies.
Ainsworth, E.A., Gillespie, K.M., 2007. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent. Nat. Protocols 2, 875-877.
Albach, D.C., Grayer, R.J., Kite, G.C., Jensen, S.R., 2005. Veronica: Acylated flavone glycosides as chemosystematic markers. Biochem. Syst. Ecol. 33, 1167-1177.
Alu’datt, M.H.V., Alli, I., Ereifej, K., Alhamad, M.N., Alsaad, A.V., Rababeh, T., 2011. Optimisation and characterisation of various extraction conditions of phenolic compounds and antioxidant activity in olive seeds. Nat. Prod. Res. 25, 876-889.
Chahmi, N., Anissi, J., Jennan, S., Farah, A., Sendide, K., El Hassouni, M., 2015. Antioxidant activities and total phenol content of Inula viscosa extracts selected from three regions of Morocco. Asian Pac. J. Trop. Biomed. 5, 228-233.
Cos, P., Vlietinck, A.J., Berghe, D.V., Maes, L. 2006. Anti-infective potential of natural products: how to develop a stronger in vitro ‘proof-of-concept’. J. Ethnopharmacol. 106(3), 290-302.
Fischer, M. A. 1981: Veronica. In Rechinger, K. H (Ed.): Flora Iranica 147: 25-165. - Akad. Druck. V. -verlagsanstalt, Graz.
Ghahreman, A., Naqinezhad, A., Atar, F., Hamzehei, B., Asadi, M., 2006. The flora of threatened black alder forests in the Caspian lowlands, northern Iran. Rostaniha, 7, 1-26.
Grundemann, C., Garcia-Kaufer, M., Sauer, B., Stangenberg, E., Konczol, M., Merfort, I., Zehl, M., Huber, R., 2013. Traditionally used Veronica officinalis inhibits proinflammatory mediators via the NF-kB signalling pathway in a human lung cell line. J. Ethnopharmacol. 145(1), 118-126.
Gusev, N., Nemereshina, O., Petrova, G., Sychev, M., 2012. Evaluation of biologically active substances and antibacterial activity of herbal drugs from Veronica L. Rus. J. Biopharm. 4, 17-22.
Harput, U.S., Saracoglu, I., Inoue, M., Ogihara, Y., 2002. Anti-inflammatory and cytotoxic activities of five Veronica species. Biol. Pharm. Bull. 25, 483-486.
Harput, U.S., Genç, Y., Khan, N., Saracoglu, I., 2011. Radical scavenging effects of different Veronica species. Rec. Nat. Prod. 5(2), 100-107.
Ignjatović, Đ., Živković, J., Tovilović, G., Šavikin, K., Tomić, M., Maksimović, Z., Janković, T., 2015. Evaluation of angiogenic and neuroprotective potential of different extracts from three Veronica species. Front. Life Sci. 8(2), 107-116.
Jayanthi, P., Lalitha, P., 2011. Reducing power of the solvent extracts of Eichhornia crassipes (Mart.) Solms. Int. J. Pharm. Pharm. Sci. 3, 126-128.
Mohadjerani, M., 2012. antioxidant activity and total phenolic content of Nerium oleander L. grown in north of Iran. Iran. J. Pharm. Res. 11, 1121.
Mosmann, T., 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 65, 55-63.
Salmanian, S., Sadeghi Mahoonak, A., Alami, M., Ghorbani, M., 2014. Phenolic content, antiradical, antioxidant, and antibacterial properties of Hawthorn (Crataegus elbursensis) seed and pulp extract. J. Agric. Sci. Technol. 16, 343-354.
Saracoglu, I., Harput, U.S., 2012. In vitro cytotoxic activity and structure activity relationships of iridoid glucosides derived from Veronica species. Phytother. Res. 26, 148-152.
Stojković, D.S., Živković, J., Soković, M., Glamočlija, J., Ferreira, I.C., Janković, T., Maksimović, Z., 2013. Antibacterial activity of Veronica montana L. extract and of protocatechuic acid incorporated in a food system. Food Chem. Toxicol. 55, 209-213.
Taskova, R.M., Gotfredsen, C.H., Jensen, S.R., 2006. Chemotaxonomy of Veroniceae and its allies in the Plantaginaceae. Phytochemistry 67, 286-301.
Vahdati, F. B., Mehrvarz, S. S., Naqinezhad, A., Gholizadeh, H., 2014. How plant diversity features change across ecological species groups? A case study of a temperate deciduous forest in northern Iran. Biodiversitas 15(1), 31-38.
Živković, J., Ćebović, T., Maksimović, Z., 2012. In vivo and in vitro antioxidant effects of three Veronica species. Open Life Sci. 7, 559-568.
Živković, J., Barreira, J.C., Stojković, D., Ćebović, T., Santos-Buelga, C., Maksimović, Z., Ferreira, I.C., 2014. Phenolic profile, antibacterial, antimutagenic and antitumour evaluation of Veronica urticifolia Jacq. J. Funct. Foods 9, 192-201.