Number 2 Volume 1

Open Access Article

1 - The effect of probiotics on gastrointestinal disease
Soheila Fadaei

Probiotics are a group of beneficial microorganisms living in the gut, which when consumed have a positive effect on physiological health directly or indirectly through the modulation of the internal ecosystem influencing the results for the use of probiotics. Indicate More

Probiotics are a group of beneficial microorganisms living in the gut, which when consumed have a positive effect on physiological health directly or indirectly through the modulation of the internal ecosystem influencing the results for the use of probiotics. Indicated as a treatment for gastrointestinal disorders are 1) the use of Saccharomyces boulardi Enteroccus faecium SF68 to prevent or shorten the duration of antibiotic-associated diarrhea, 2) the prevention of S. boulardi, which prevents Clostridium difficil-associated diarrhea. 3) Fermented milk contains Lactobacillus rhamnsousder for infants with rotavirus enteritis. Therefore, according to the researchers, the use of probiotics, the creation of microbial recombination of the intestines, gastroenteritis, lactose intolerance and diarrhea are related to the use of antibiotics from these biological substances as a treatment for diseases caused by chronic diseases. Manuscript profile

Open Access Article

2 - Antibiotic resistance pattern and prevalence of class 1, 2 and 3 integron genes in Escherichia coli strains isolated from urinary tract infections in Shahrekord
Marziyeh Soleymanian نازیلا ارباب سلیمانی sanaz khaksar haghani

Integrons are mobile genetic elements capable of carrying resistance genes to various antibiotics. These elements have been found in different places of plasmid and chromosome. The aim of this present study was determine the prevalence of class 1, 2 and 3 integrons in E More

Integrons are mobile genetic elements capable of carrying resistance genes to various antibiotics. These elements have been found in different places of plasmid and chromosome. The aim of this present study was determine the prevalence of class 1, 2 and 3 integrons in Escherichia coli isolates isolated from urinary tract infection in Shahrekord. In this research, the number of 64 isolates of Escherichia coli were investigated. The antibiotic resistance of the investigated isolates was evaluated using a simple disking method in Mueller Hinton agar medium. In order to determine the frequency of class 1, 2 and 3 integrons, specific primer pairs were used. After the antibiogram test, the highest resistance to ampicillin (75%) and the lowest resistance to imipenem (12.5%) were observed. The frequency of class 1, 2 and 3 integron genes was observed as 12.5%, 6.25% and 3.12%, respectively. None of the integron genes were observed in 52 isolates. In the statistical analysis with chi-square test, a statistically significant relationship was observed between class 1 integron and resistance to the antibiotic ampicillin (p = 0.02 < 0.05). Due to the fact that resistance genes are located on integrons and can be transferred from one strain to another strain and spread resistance in the hospital or other environments, this has doubled the importance of identifying this type of antibiotic resistance genes. Key words: Escherichia coli, integron, antibiotic resistance, urinary infection Manuscript profile

Open Access Article

3 - Determine the antibiotic resistance patterns and tsst-1 gene frequency in staphylococcus auresu strains isolated from patients of Imam Khomeini hospital in Ahvaz
ziba shanaki baversad مریم ریسی Marziyeh Soleymanian

isolates of Staphylococcus aureus from different samples to determine antibiotic resistance and Staphylococcus aureus is a major cause of hospital acquired infections and community. Toxic shock syndrome toxin -1 gene secreted by the bacteria from the categories are impo More

isolates of Staphylococcus aureus from different samples to determine antibiotic resistance and Staphylococcus aureus is a major cause of hospital acquired infections and community. Toxic shock syndrome toxin -1 gene secreted by the bacteria from the categories are important virulence factors and is component super antigens toxins pyrogenic (PTSAgs). The purpose of this study is determine the antibiotic resistance patterns and tsst-1 gene frequency in Staphylococcus aureus strains isolated from patients of Imam Khomeini hospital in Ahvaz. In this study, 133 clinical frequency tsst-1 gene were studied. After genomic DNA extraction using DNA extraction kit was performed the definitive diagnosis of bacteria, Then the gene tsst-1 frequency done in the presence of specific primers and antibiotic resistance was determined by agar disk diffusion method. After PCR amplification and detection of the bacterium, Of 133 isolates sequence tsst-1 gene was observed in 6 strains. In antibiogram test the greatest resistance to cefazolin (3/83%) and the lowest resistance to nitrofurantoin and vancomycin (0%) was observed. Due to the increasing prevalence of resistance to antibiotics of clinical importance tsst-1 gene timely identification and implementation of appropriate therapeutic strategies for controlling infection seems necessary. Manuscript profile

Open Access Article

4 - Chaperones, vital molecules in microbes
Kimia Golestanfar

Molecular chaperones are highly conserved proteins that promote proper folding of other proteins inside the body. Diverse chaperone systems contribute to protein folding and translocation, assembly of oligomeric complexes, and recovery from stress-induced unfolding. A f More

Molecular chaperones are highly conserved proteins that promote proper folding of other proteins inside the body. Diverse chaperone systems contribute to protein folding and translocation, assembly of oligomeric complexes, and recovery from stress-induced unfolding. A fundamental function of molecular chaperones is to inhibit nonproductive protein interactions by recognizing and protecting hydrophobic surfaces that are exposed during folding or following proteotoxic stress. Therefore, chaperones are of special importance in cellular systems, which are discussed in this review article about these molecules and their mechanisms of action. Also, changes in gene expression in oxidative conditions in bacteria will be discussed in order to tolerate environmental conditions. Manuscript profile

Open Access Article

5 - Investigating and identifying Vibrio contamination of farmed fish and shrimps in Khuzestan province
reza soltani Zahra Motaghi Hossein Khodabandeh shahraki

Vibriosis is one of the important common diseases between farmed fish and humans in the aquaculture industry. This bacterium belongs to the Vibrionaceae family and includes curved, motile, facultatively anaerobic chemoorganotroph, catalase and oxidase positive bacilli. More

Vibriosis is one of the important common diseases between farmed fish and humans in the aquaculture industry. This bacterium belongs to the Vibrionaceae family and includes curved, motile, facultatively anaerobic chemoorganotroph, catalase and oxidase positive bacilli. The purpose of this research is to investigate the possible presence of Vibrio bacteria in farmed salmon, carp and shrimp. For this purpose, 60 pieces of salmon, carp and shrimp were caught from the breeding farms of Khuzestan province and transported to the laboratory of the food research center of Azad University, Shahrekord branch, next to the ice. Then, the liver and ventricular area were cultured on thiosulfate citrate bile sucrose agar (TCBS), the environments were kept in a greenhouse for 48 to 72 hours at 25°C, and then based on the presence or absence of grown colonies , ..... percentage of the studied fish meat (... pieces) were infected with vibrio, which was very noteworthy. According to the previous investigations and the results of this study, the monitoring and control of the production chain and the timely diagnosis of this organism during this chain can play a significant role in reducing the contamination of marine products, including fish. This will ultimately reduce the incidence of this disease in the community Manuscript profile

Open Access Article

6 - Investigation of the contamination of semi-prepared foods and fast foods with respect to Staphylococcus aureus bacteria in Chaharmahal and Bakhtiari province.
Manouchehr Momeni-Shahraki fatemeh shirazi یاس محمدی Hossein Khodabandeh shahraki

Fast and cold foods increase the microbiological risk of consumers due to the lack of cooking time and the contact of restaurant workers' hands during preparation. Staphylococcus aureus is one of the most important causes of foodborne diseases. The purpose of this study More

Fast and cold foods increase the microbiological risk of consumers due to the lack of cooking time and the contact of restaurant workers' hands during preparation. Staphylococcus aureus is one of the most important causes of foodborne diseases. The purpose of this study is to determine the abundance of Staphylococcus aureus in semi-prepared foods and fast foods and to identify them by PCR method. In this research, 100 food samples (meat products, falafel, pizza, chicken) were randomly collected from the food preparation and serving centers in Borujen, Shahrekord and Farsan cities in a period of one month to investigate the presence of Staphylococcus aureus. Tested. In total, staphylococcus contamination was observed in samples of meat products (64.1%), falafel (21.2%), pizza (20%), chicken (30.8%). The average number of Staphylococcus aureus bacteria in positive samples was reported (39%). Based on the results of the present research, it can be said that since the results of the production steps and preparation of fast food and semi-prepared foods are manual, there is a possibility of food contamination through human labor. Although the percentage of sample contamination is high, the number of bacteria in the sample is low and does not pose a potential risk to the health of the consumer. Therefore, personal and environmental health education is of great importance in order to reduce the amount of Staphylococcus aureus in people who are involved in food preparation and production. Manuscript profile

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