Investigating and identifying Vibrio contamination of farmed fish and shrimps in Khuzestan province
Subject Areas : Food microbiologyreza soltani 1 , Zahra Motaghi 2 , Hossein Khodabandeh shahraki 3
1 - . دانشجوی دکتری بهداشت مواد غذایی، دانشکده دامپزشکی، دانشگاه آزاد اسلامی، واحد شهرکرد، شهرکرد، ایران
2 -
3 -
Keywords: rainbow trout, carp, shrimp, vibrio, joint disease,
Abstract :
Vibriosis is one of the important common diseases between farmed fish and humans in the aquaculture industry. This bacterium belongs to the Vibrionaceae family and includes curved, motile, facultatively anaerobic chemoorganotroph, catalase and oxidase positive bacilli. The purpose of this research is to investigate the possible presence of Vibrio bacteria in farmed salmon, carp and shrimp. For this purpose, 60 pieces of salmon, carp and shrimp were caught from the breeding farms of Khuzestan province and transported to the laboratory of the food research center of Azad University, Shahrekord branch, next to the ice. Then, the liver and ventricular area were cultured on thiosulfate citrate bile sucrose agar (TCBS), the environments were kept in a greenhouse for 48 to 72 hours at 25°C, and then based on the presence or absence of grown colonies , ..... percentage of the studied fish meat (... pieces) were infected with vibrio, which was very noteworthy. According to the previous investigations and the results of this study, the monitoring and control of the production chain and the timely diagnosis of this organism during this chain can play a significant role in reducing the contamination of marine products, including fish. This will ultimately reduce the incidence of this disease in the community
منابع
1.آخوندزاده بستی از ابراهیم زاده موسوی، ح. میثاقی، ع سلطانی .م. اسماعیلی، ح. (۱۳۸۶). بررسی گونه های ویبریو در میگوهای پرورشی Paeneus indicus و دریایی semisulcatus Paeneus صید شده در استان بوشهر مجله تحقیقات دامپزشکی 62 (5) :310-307
2.رضویلر، و (۱۳۸۷) میکروبهای بیماری را در مواد غذایی و اپیدمیولوژی مسمومیتهای غذایی انتشارات دانشگاه تهران، ۱۱۱-۱۱۵
3.سلطانی، م. کاکولکی، ش. آوخ کیسمی، م. (۱۳۷۹). جداسازی و شناسایی گونه های غالب ویبریو در میگوهای پرورشی تعدادی از کارگاههای پرورش میگوی بوشهر مجله دانشکده دامپزشکی دانشگاه تهران. ۵۵ (۲): ۳۲- ۲۹
4.اخلاقی، م ،حقیقی ز منصوری، ه. (۱۳۹۱). مطالعه ایمنوسیتوشیمی ،کبد، طحال و روده ماهی قزل آلای رنگین کمان ایمن شده با آنتی ژن ویبریو آنگوئیلاروم مجله تحقیقات دامپزشکی. ۶۷ (۲): ۱۹۷- ۱۹۱
5. Ansari M, Raissy M (2010). In vitro susceptibility of commonly used antibiotics against isolated from Lobster (Panulirushomarus). Af. J. Microb. Res., pp. 629-631. Vibrio
6. Austin B; Austin DA. (2007) Bacterial fish pathogens, disease of farmed and wild fish, 4rd ed. Springer, Praxis Publishing, UK.
7. Chrisolite B, Thiyagarajan S, Alavandi SV, Abhilash EC, Kalaimani N, Vijayan KK, Santiago TC (2008). Distribution of luminescent Vibrio harveyi and their bacteriophages in a commercial shrimp hatchery in South India. Aquaculture, 275: 13-19.
8. Di Pinto A, Ciccarese G, Tantillo G, Catalano D, Fortel VT (2005). A Collagenase- Targeted Multiplex PCR Assay for Identification of Vibrio alginolyticus, Vibrio cholera and Vibrio parahaemolyticus. J. Food. Prot., 68: 150-153.
9. Garrity GM; Holt JG.(2001) The road map to the manual. In; Brgey's manual of systematic bacteriology. 2nd ed, Vol. 1 (edsD. R Boone and R. W Castenbolz). Springer, New York.
10. Hosseini H, Cheraghali MA, Yalfani R, Razavilar V (2004). Incidence of Vibrio spp. in shrimp caught off the south coast of Iran. Food Control., 15: 187-190.
11.Gopal, S., Otta, S.K., Kumar, S., Karunasagar, I., Nishibuchi, M. and Karunasagar, I. (2005).
Occurrence of Vibrio species in tropical shrimp culture environment, implication for food safety.
International Journal of Food Microbiology, 102: 151-159.
12.Oliver, J.D. and Japer, J.B. (1997).Vibrio species. In: Doyle MP, Beuchat L.R and Montville T.J.
(Eds.), Food Microbiology – Fundamentals and Frontiers. Washington DC: ASM Press, pp. 228-264.
13.Baffone, W., Pianetti, A., Bruscolini, F., Barbieri, E. and Citterio, B. (2000). Occurrence and
expression of virulence-related properties of Vibrio species isolated from widely consumed sea food
products. International Journal of Food Microbiology, 54: 9-18.
14.Codex Alimentarius (2009). Code of practice for fish and fishery products, First edition, FAO and
WHO, Rome.
15. Kvenberg, E.J. (1991). Non-indigenous Bacterial Pathogens, In: Microbiology of Marine Food
Products. (Eds.), Donn RW, Cameron H Van Nostrand Reinhold, New York, pp. 263-291.
16. Rodricks, E.G. (1991). Indigenous Pathogen: Vibrionaceae In: Microbiology of Marine Food
Products. (eds) Donn, R. W. and Cameron, H. Van Nostrand Reinhold, New York, pp. 285-295.
17. Tarr CL, Patel JS, Puhr ND, Sowers EG, Bopp CA, Strockbine NA. Identification of Vibrio isolates by a multiplex PCR assay and rpoB sequence determination. J Clin Microbiol. 2007;45(1):134-40.
18. Bej AK, Patterson DP, Brasher CW, Vickery MCL, Jones DD, Kaysner CA. 1999. Detection of total and hemolysin producing Vibrio parahaemolyticus in shellfish using multiplex PCR amplification of tlh, tdh and trh. J Microbiol Method. 1999;36(3):215–225.
19. Zhou, S., Hou, Z., Li, N and Qin, Q. (2007) Development of a SYBR Green I real-time PCR for quantitative detection of Vibrio alginolyticus in seawater and seafood, J Appl Microbiol. 103:1897-1906. PMID: 17953599.
20.Shirazi MH, Ranjbar R, Salari MH, Bagheri Tirtash Y, Najafi A, Sadeghifard, N. Isolation of Vibrio parahaemolyticus from fish in Tehran and their antimicrobial resistance. Iranian J Infect Diss Trop Med. 2007;11(35):65-68.
21.Jalali jafari B, Barzegar dolatabadi M. Shrimp health management. 2nd ed. Tehran: Noorbakhsh Publications; 2009.
22.Mahmud Z, Kassu A, Mohammad A, Yamato M, Bhuiyan NA, Balakrish Nair G, et al. Isolation and molecular characterization of toxigenic Vibrio parahaemolyticus from the kii Channel, Japon. Microbiol Res. 2006;161(1):25-37.
23.Ottaviani D, Santarelli S, Bacchiocchi S, Masini L, Ghittino C, Bacchiocchi I. Presence of pathogenic Vibrio parahaemolyticus strains in mussels from the Adriatic Sea, Italy. Food Microbiol. 2005;22(6):585-590.
24.Senachai P, Chomvarin C, Namwat W, Wongboot W, Wongwajana S, Tangkanakul W. Application of tetraplex PCR for detection of Vibrio cholerae, V. parahaemolyticus, V. vulnificus and V. mimicus in cockle. Southeast Asian J Trop Med Publ Health. 2013;44(2):249-58.
25.Maheshwari M, Krishnaiah N, Ramana V. Evaluation of Polymerase Chain Reaction for the detection of Vibrio cholerae in Contaminants. Ann Biol Res. 2011;2(4):212-217.