This study was performed to evaluate the effects of folic acid (FA) and methionine (Mt) administration on egg 5-methyltetrahydrofolate (5-MTHF) content, levels of zinc in yolk and serum and concentration of homocysteine on Hy-line W36 hens from 20 to 26 week
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This study was performed to evaluate the effects of folic acid (FA) and methionine (Mt) administration on egg 5-methyltetrahydrofolate (5-MTHF) content, levels of zinc in yolk and serum and concentration of homocysteine on Hy-line W36 hens from 20 to 26 weeks of age. The experiment was conducted as a 4×4 factorial arrangement of treatments in randomized complete design with four levels of Mt (0.25, 0.32, 042, 0.48 mg/kg of diet) and four levels of FA (0, 5, 10, 15 mg/kg of diet). Fortification of laying hens diets with FA 0, 5, 10 and15 mg/kg of diet increased 5-MTHF into yolk significantly (p<0.05). 5-MTHF content of egg was also significantly affected by FA×Mt interaction (p<0.05). FA significantly reduced Zn level of egg and serum (p<0.05), but Mt did not have any effect on Zn content in egg. Levels of serum homocysteine were significantly reduced by fortification of diet by FA (p<0.05).Supplementation of laying poultry diet with FA and Mt could improve the content of yolk 5-MTHF.
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