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Open Access Article
1 - Growth and Mortality Parameters of Kawakawa (Euthynnus affinis) in Coastal Hormozgan (Persian Gulf and Sea of Oman)
S.A.R. Hashemi S.A. Taghavi P. Kochanian H. Pasha Sanoosi Euthynnus affinis with Common name Kawakawa is an important species in scombrid fishery in the Hormozgan and southern proviences in Iran. So, study of growth and mortality parameters of this species in t More Euthynnus affinis with Common name Kawakawa is an important species in scombrid fishery in the Hormozgan and southern proviences in Iran. So, study of growth and mortality parameters of this species in the region is a very crucial factor for catch management. In this research according to migration behavior of kawakawa in Hormozgan, Samples were collected from landing at four stations: Gavbandy, Kong, Salakh and Gask. During the three-year study, from 2003 to 2005, more than 25000 specimen were measured, mean±S.D Values for each year were 64/58±6/27, 64/73±7/2, and 62/96±5/31 respectively. The maximum and minimum total lengths were 25cm and 86cm respectively. Growth and mortality parameters were calculated for the 3 years as below, L∞: 89, 87 and 87 and K: 0.5, 0.52 and 0.53 and t0: -0.30,- 0.46, and -0.48, Z: 1.78, 2.72 and 2.62, M: 0.64, 0.66 and 0.67, F: 1.14, 2.06 and 1.95 respectively. Exploitation ratio (E) was estimated 0.64, 0.75 and 0.74 respectively and according to exploitation coefficient E. affinis stock is over fishing and deaceares exploitation coefficient proposed. Based on the result and in comparison with to American fisheries society (AFS) indices, Kawakawa is classified as moderate vulnerable group fishes. Manuscript profile -
Open Access Article
2 - Genetic comparison of Persian Gulf and Oman Sea populations of the Cobia, Rachycentron canadum by means of microsatellite technique
M.A. Salari Aliabadi S. Rezvani Gilkolaei A. Savari H. Zolgharnian S.M.B. Nabavi In this study genetic diversity of Cobia, Rachycentron canadum populations were assessed using microsatellite technique in the northern coasts of Persian Gulf (Booshehr, Dayer Port, Lengeh Port & Ban More In this study genetic diversity of Cobia, Rachycentron canadum populations were assessed using microsatellite technique in the northern coasts of Persian Gulf (Booshehr, Dayer Port, Lengeh Port & Bandarabass) and Oman Sea (Pozm & Beris of Chabahar). Polymerase chain reactions (PCR) were conducted on the target DNA using 10 paired microsatellite primers. PCR products were electrophoresed on polyacrylamid gels (8%) that were stained using silver nitrate. Diagram of genetic distance was calculated using the UPGMA method in TFPGA version 1.3 for any level of the hierarchy. The results showed that the mean of observed and effective allele number was 12.357 and 8.319 respectively. Mean observed and expected heterozygosity was 0.655 and 0.874 respectively. Based on Analysis of Molecular Variance (AMOVA) the highest Fst (0.063) was observed when comparing specimens from Dayer Port zone and Pozm of Chabahar zone (Nm=3.7). The highest genetic distance (0.815) was observed between specimens from Dayer Port zone and Beris of Chabahar zone. The result obtained from the present study shows that at least 3 different population of R. canadum are found in the northern coasts of Persian Gulf and Oman Sea, which are including: Booshehr region population, Bandarabass region population, and Chabahar region population. Specific markers were also identified for of the Booshehr zone population identified. The Booshehr population zone can be identified using primers Rca 1B-E08A, Rca 1B-F07, Rca 1B-H09 and Rca 1-A04. Manuscript profile -
Open Access Article
3 - Consideration of crude oil pollution (Water Soluble Fraction) in lelation to PAHs accumulation in Cynoglossus bilineatus of Persian Gulf
Sh. Oryan M. Gharibkhany M. Tatina One of the compounds of crude oil is Polycyclic Aromatic Hydrocarbons(PAHs). This study was conducted in order to consider the effect of water soluble fraction of crude oil on the accumulation of PAHs in muscle tissue of Cynoglossus bilineatus of Pers More One of the compounds of crude oil is Polycyclic Aromatic Hydrocarbons(PAHs). This study was conducted in order to consider the effect of water soluble fraction of crude oil on the accumulation of PAHs in muscle tissue of Cynoglossus bilineatus of Persian Gulf. It was performed in spring 2005 in the ecology research center of Persian Gulf. Two solutions with 0ppm and 18.25ppm dosages of crude oil were prepared through by Anderson method and the use of seawater. Then 48 fish caught from different stations of Persian Gulf were exposed (to 8 fish for each dosage) for 8 days in long-term bioassay examination with three replicates. After 8 days, exposed fish were frozen and packed seprately and sent to the laboratory for determination of PAHs accumulated in their muscle. PAHs measurment was done with GC-FID model varian 3400. In total 16 different compound of PAHs were detected. Then total PAHs in each dosage was calculated seprately. The result of data analysis shows that mean accumulation amount of PAHs (STD) in tissue of testifier fish (0 ppm dosage) and exposed to 18.25 ppm dosage of crude oil was 1622±13 ppb and 3983±9.16 ppb respectively. According to Kruskal-wallis test there is a significant difference (P<0.05) between two mentioned dosages of crude oil from their accumulation point of view in muscle tissue. In other words exposing fish to 18.25ppm of crude oil can increase the accumulation of PAHs in comparition with testifier fish. Manuscript profile -
Open Access Article
4 - Determination of normal values of some blood serum factors (Electrolyte and non electrolyte) of Acipenser persicus
S.A. Shahidi Yasaghi M. Mazandarani A. Ghorbani Hasan Saraei R. Ghorbani N. SoleimaniHematology and Biochemical analysis of the serum is an important method in diagnosis of fish diseases. 12 Biochemical parameters of the serum of Acipenser persicus including: Sodium (Na), Potassium (K), Calcium (Ca), Phosphorus (P), Glucose, Blood Urea Nitrogen (BUN), C MoreHematology and Biochemical analysis of the serum is an important method in diagnosis of fish diseases. 12 Biochemical parameters of the serum of Acipenser persicus including: Sodium (Na), Potassium (K), Calcium (Ca), Phosphorus (P), Glucose, Blood Urea Nitrogen (BUN), Creatinine, Cholesterol, Triglyceride, Bilirubin, Albumin and Total protein have been evaluated in this study. Sampling (Bleeding) carried out in spring (March) from south east of Caspian Sea. Samples were taken from peduncle of male (16 samples) and female (16 samples) Persian sturgeon (Acipenser persicus). Samples were carried to the lab in an ice bag, then serum was separated and the content was measured by an Auto analyzer (RA 1000) and flame photometer (405C). Results were statistically compared by the method of t-test between male and female. Among these result only P content in female samples were significantly higher than that of male (P<0/05) .According to pearson,s correlation, There is a significant correlation between cholesterol and Albumin, Cholesterol and Triglyceride, and phosphorus, Calcium and total protein, Calcium and phosphorus, Albumin and Total protein (P<0/05). Manuscript profile -
Open Access Article
5 - Effect of feeding by yeast (Saccharomyces cerevisiae) as a probiotic, in contrast with salinity stress and on intestinal histology in rainbow trout (Oncorhynchus mykiss) fry
M. Pooramini A. Kamali A. Hajimoradloo R. Ghorbani M. Alizadeh Improvement of growth and resistance against the environmental stress is one of the main functions of probiotics. In this study, the strain of Saccharomyces cerevisiae var. elipsoidous was tested as a pr More Improvement of growth and resistance against the environmental stress is one of the main functions of probiotics. In this study, the strain of Saccharomyces cerevisiae var. elipsoidous was tested as a probiotic in the diet of rainbow trout (Oncorhynchus mykiss) fry (163±26.27mg) during 25 days after yolk absorption. The yeast was added to the diet plates in three levels (1, 5, 10% w/w) and with three triplications. Besides, a treatment with cod oil (without yeast) was used and the results were compared with that of control group. The yeast effect on rainbow trout fry mortality was not significant along the rearing period. Histological finding showed no difference in the treatments too. Challenging with different levels of salinity after 24 hours showed that treatments contained yeast as a probiotic had 100% survival (p>0.05) and showed significant differences with cod oil treatment (without yeast) and control (p<0.05). Manuscript profile -
Open Access Article
6 - ماکروبنتوز، نهر مادرسو، فراوانی
S. Jorjani A. Ghelichi R. Akrami V. KheirabadiThis study was conducted to identification of macrobenthoses in Madarsu Stream to evaluation of quality of water. Sampling was being done from April 2006 to March 2007. Macrobenthoses samples were collected by Surber sampler (30×30cm). The collected benthoses were MoreThis study was conducted to identification of macrobenthoses in Madarsu Stream to evaluation of quality of water. Sampling was being done from April 2006 to March 2007. Macrobenthoses samples were collected by Surber sampler (30×30cm). The collected benthoses were fixed in 4% formaldehyde solution and transferred to the laboratory. In each station while sampling, discharge and water temperature were also measured. The most frequent orders were Ephemeroptera, Trichoptera and Diptera. Among Diptera three families of Chironomidae, Simulidae and Tabanidae had been dominant with the frequency of 64, 17 and 17 percents respectively. The maximum number of Ephemeroptera and Trichoptera was seen in mid August, while in Diptera the maximum was observed in May and early February. The decrease in the number of Diptera could have been because of water high discharge during March. Considering frequency of benthic fauna in different months, Ephemeroptera was dominant. But in some station (esp. the 5th one) because of entering of Tangerah village sewage of restaurants to this part of stream Diptera was dominant. The number of Diptera (especially Diptera) had a considerable decrease during summer. It could have been because of vertical migration of this benthos from bottom to surface and feeding activity of benthos feeders in particular. Because of Madarsu bed-stream which is rubble-stony in most places, low amount of organic materials in water, there were not differences in stations. To sum it up, the water in Madarsu stream has high quality. Occurrence of seasonal floods during early August causes significant decrease in the number of macrobenthoses and diversity in the stream reduced because of occurrence of floods in the past years. Manuscript profile -
Open Access Article
7 - The effect of recombinant bovine somatotropin (Eltosil®) on growth performance of rainbow trout (Oncorhynchus mykiss)
M. Haghighi M. Sharif Rohani I. Sharifpour A. SepahdariThe objective of this study was to evaluate the effect of recombinant bovine somatotropin 1% (Eltosil®) on growth performance in rainbow trout. 1200 rainbow trout (Oncorhynchus mykiss) weighing 0.5±0.05 gram were randomly distributed in two groups 1) the cont MoreThe objective of this study was to evaluate the effect of recombinant bovine somatotropin 1% (Eltosil®) on growth performance in rainbow trout. 1200 rainbow trout (Oncorhynchus mykiss) weighing 0.5±0.05 gram were randomly distributed in two groups 1) the control group and 2) Eltosil® group, each in three replications. These fishes were randomly divided in six 100 liter square troughs with a continuous water flow of 1 liter per minute. Troughs were supplied with a well water (temperature range: 15±1ºC, O2: 7.2±0.2mg/l, pH= 8±0.3). In this research, the fish received of 200g Eltosil® per 100kg BW. The administration method of Eltosil®-mixed feed to fish was once a week for 4 consecutive weeks at each treatment stage, totally 8 times for 12 weeks (once a week, 4 times in the first step, withdrawal for four weeks in the second step, once a week for 4 times in the third step). To calculate growth factors, fish were weighed weekly and their body lengths were measured every two weeks (before and after each experiment). At the end of each rearing period (on day 28, 56, 84) the means of whole body weight, weight gain, average daily growth, total length, average daily length, grams of weight gain per gram of food, specific growth rate, condition factor, food conversion rate, and the percentage of survival ratio were calculated in the control and Eltosil® groups. Moreover, at the end of rearing period (on day 84), comparative growth rate was calculated. The results of this study demonstrated that there were no significant differences between growth factors in the control and Eltosil® groups on days 28, 56, 84 of rearing periods (P>0.05). These results show that oral administration of recombinant bovine somatotropin (Eltosil®) was not a suitable way to increase rapid growth in rainbow trout weighing 0.5±0.05 gram. Manuscript profile -
Open Access Article
8 - Survay the Coliform pollution in the Shafarood river of Guilan Province
S. Khatib haghighi A. Ghane M. Nahrvar In this survey, in order to study the Coliform pollution of Shafarud River, two layers from surface and sediment were sampled in five stations. Sampling was done seasonally during a year. The pollution b More In this survey, in order to study the Coliform pollution of Shafarud River, two layers from surface and sediment were sampled in five stations. Sampling was done seasonally during a year. The pollution by standard diagnosis of Coliform was completely examined. The most pollution was found in sediment layer of delta area. Besides, the highest Coliform count was seen during summer amounting to (44.2) Coliform in 100 cm3 and Fecal Coliform (22.1) in 100 cm3 river water. In summer, due to the high temperature, growth of bacteria is more than ever and in the delta area, because of calm flowing of water and it is passing through the city and agricultural centers, and entering garbage into it, the amount of pollution has been apparently reached a high level in comparison with other parts of the rivers with rapid flowing of water. Manuscript profile
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