Consideration of crude oil pollution (Water Soluble Fraction) in lelation to PAHs accumulation in Cynoglossus bilineatus of Persian Gulf
Subject Areas : New Technologies in Aquaculture DevelopmentSh. Oryan 1 , M. Gharibkhany 2 , M. Tatina 3
1 - Dept. of Animal Biology, Tarbiat Moalem University, Tehran, Iran
2 - Dept. of Fisheries of Islamic Azad University, Astara Branch, Iran
3 - Dept. of Fisheries of Islamic Azad University, Astara Branch, Iran
Keywords: Crude oil, Persian Gulf, PAHs, Cynoglossus bilineatus,
Abstract :
One of the compounds of crude oil is Polycyclic Aromatic Hydrocarbons(PAHs). This study was conducted in order to consider the effect of water soluble fraction of crude oil on the accumulation of PAHs in muscle tissue of Cynoglossus bilineatus of Persian Gulf. It was performed in spring 2005 in the ecology research center of Persian Gulf. Two solutions with 0ppm and 18.25ppm dosages of crude oil were prepared through by Anderson method and the use of seawater. Then 48 fish caught from different stations of Persian Gulf were exposed (to 8 fish for each dosage) for 8 days in long-term bioassay examination with three replicates. After 8 days, exposed fish were frozen and packed seprately and sent to the laboratory for determination of PAHs accumulated in their muscle. PAHs measurment was done with GC-FID model varian 3400. In total 16 different compound of PAHs were detected. Then total PAHs in each dosage was calculated seprately. The result of data analysis shows that mean accumulation amount of PAHs (STD) in tissue of testifier fish (0 ppm dosage) and exposed to 18.25 ppm dosage of crude oil was 1622±13 ppb and 3983±9.16 ppb respectively. According to Kruskal-wallis test there is a significant difference (P<0.05) between two mentioned dosages of crude oil from their accumulation point of view in muscle tissue. In other words exposing fish to 18.25ppm of crude oil can increase the accumulation of PAHs in comparition with testifier fish.
_||_