Genetic comparison of Persian Gulf and Oman Sea populations of the Cobia, Rachycentron canadum by means of microsatellite technique

Subject Areas : New Technologies in Aquaculture Development

M.A. Salari Aliabadi ¹ , S. Rezvani Gilkolaei ² , A. Savari ³ , H. Zolgharnian ⁴ , S.M.B. Nabavi ⁵

1 - College of Marine Science, Dept. of Marine Biology, Khorramshahr University of Marine Science and Technology, Khorramshahr, Khuzestan, Iran
2 - Iranian Fisheries Research Institute, Tehran, Iran
3 - College of Marine Science, Dept. of Marine Biology, Khorramshahr University of Marine Science and Technology, Khorramshahr, Khuzestan, Iran
4 - College of Marine Science, Dept. of Marine Biology, Khorramshahr University of Marine Science and Technology, Khorramshahr, Khuzestan, Iran
5 - College of Marine Science, Dept. of Marine Biology, Khorramshahr University of Marine Science and Technology, Khorramshahr, Khuzestan, Iran

Received: 2019-11-06 Accepted : 2019-11-06 Published : 2008-05-21

Keywords: Microsatellite, Persian Gulf, Oman Sea, Rachycentron canadum, Cobia, populations genetic,

Abstract :

            In this study genetic diversity of Cobia, Rachycentron canadum populations were assessed using microsatellite technique in the northern coasts of Persian Gulf (Booshehr, Dayer Port, Lengeh Port & Bandarabass) and Oman Sea (Pozm & Beris of Chabahar). Polymerase chain reactions (PCR) were conducted on the target DNA using 10 paired microsatellite primers. PCR products were electrophoresed on polyacrylamid gels (8%) that were stained using silver nitrate. Diagram of genetic distance was calculated using the UPGMA method in TFPGA version 1.3 for any level of the hierarchy. The results showed that the mean of observed and effective allele number was 12.357 and 8.319 respectively. Mean observed and expected heterozygosity was 0.655 and 0.874 respectively. Based on Analysis of Molecular Variance (AMOVA) the highest Fst (0.063) was observed when comparing specimens from Dayer Port zone and Pozm of Chabahar zone (Nm=3.7). The highest genetic distance (0.815) was observed between specimens from Dayer Port zone and Beris of Chabahar zone. The result obtained from the present study shows that at least 3 different population of R. canadum are found in the northern coasts of Persian Gulf and Oman Sea, which are including: Booshehr region population, Bandarabass region population, and Chabahar region population. Specific markers were also identified for of the Booshehr zone population identified. The Booshehr population zone can be identified using primers Rca 1B-E08A, Rca 1B-F07, Rca 1B-H09 and Rca 1-A04.