AbstractThe synthesis of extracellular polysaccharides in microorganisms, especially cyanobacteria, plays a major role in protecting the cell against many stresses in different habitats. In recent years, cyanobacterial strains belonging to the Nostocaceae family are of
More
AbstractThe synthesis of extracellular polysaccharides in microorganisms, especially cyanobacteria, plays a major role in protecting the cell against many stresses in different habitats. In recent years, cyanobacterial strains belonging to the Nostocaceae family are of interest as acceptable sources for polysaccharide production in liquid and solid suspension cultures. Therefore, in this research, the optimization of culture conditions for maximum production of extracellular polysaccharides and bioactive compounds under the influence of mixotrophic environment by filamentous cyanobacteria isolated from salt water has been studied. In the upcoming study, with the aim of investigating the effective factors in optimizing the production of exopolysaccharides and the effect of the mixotrophic environment, the cyanobacterium Aliinostoc oryzae strain was isolated from the salty waters of Golestan province, and after morphological and molecular identification, the amount of polysaccharide produced and its antimicrobial activity was investigated. The findings indicate that the amount of polysaccharide production from cyanobacteria depends on the conditions of the culture environment. The amount of polysaccharide production of cultures grown in nitrate concentration with concentrations of 0.04 and 0.06 g/ml and in light of 150 microns per square meter per second was significantly higher than the control. Antibiogram tests also showed that there was a significant difference in polysaccharide production in the presence of Staphylococcus aureus in nitrate culture. Investigations of the average production of extracellular polysaccharide extraction also showed a significant difference in different culture environments. Extracellular polysaccharide extracted from cyanobacteria has countless uses in the industry, including the pharmaceutical industry, food industry, and agriculture. Therefore, the optimization of the cyanobacterium culture conditions for the maximum production of polysaccharides appears. Since cyanobacterial exopolysaccharides are important from the point of view of biotechnology, it is important to pay attention to many other factors for maximum exopolysaccharide production in this genus of cyanobacteria.
Manuscript profile
