Introduction: Nowadays the destructive effects of oil pollutions that produced by different resourcesin waters and aquatic organisms have been defined with the intention of studying effects of crude oilin different concentration, on variations of anti-oxidant enzymes level (SOD, CAT)in Tetraclitarufotincta and the possibility of introduce these enzymes as biomarkers.Metod: Barnacles Tetraclita rufotincta were sampled in Bahrekan region. Barnacles exposed to250ppb, 125ppb, 62.5ppb, 31.25ppb, 15.625ppb and 3ppb as a control, crude oil after 24,48,72 and 96h. 15 barnacles removed from aquarium in average. Animals were examined for levels of Antioxidantsenzymes (SOD, CAT) in their tissues.Results: this study detected that in aquarium 1, with 3ppb concentration, after 24,48,72 and 96 hSuperoxide dismutase(SOD) activities shown respectively 128/98, 30/04, 75/8, 62/05U/ml/mg protein.In aquarium 2 and 3, with 15.625ppb and 31.25ppb concentration, after the period mentioned,superoxide dismutase(SOD) activities shown respectively 104/45, 74/95, 13/57, 109U/ml/mg proteinand 69/96, 61/56, 60/5, 86/46 U/ml/mg protein. In aquarium 4 and 5, with 62.5ppb and 125ppbconcentration, after the mentioned period, Superoxide dismutase(SOD) activities shown respectively98/79, 193/9, 42/75, 124/77 U/ml/mg protein and 69/22, 40/08, 81/86, 59/95U/ml/mg protein and inaquarium 6, with 250ppb concentration, after the mentioned period, Superoxide dismutase(SOD)activities shown respectively 62/11, 87/1, 20/71, 93/47U/ml/mg protein.The results also detected that in aquarium 1, with 3ppb concentration, after 24,48,72 and 96 h Catalase(CAT) activities shown respectively 13/29, 15/31, 15/5, 16/25 U/ml/mg protein. In aquarium 2 and 3,with 15.625ppb and 31.25ppb concentration, after the mentioned period, Catalase (CAT) activitiesshown respectively 13/03, 16/74, 13/65, 13/61U/ml/mg protein and 11/46, 16/54, 15/7, 13/58U/ml/mgprotein. In aquarium 4 and 5, with 62.5ppb and 125ppb concentration, after the mentioned period,Catalase (CAT) activities shown respectively 18/74, 11/86, 11/91, 16/22U/ml/mg protein and 21/1,1- PhD, Asst. Prof., Biology Dept, Islamic Azad University, Iran2- PhD, Instructor, Environment Pollution, Islamic Azad University, Iran3- PhD, Researcher, Biochemistry Dept, Research Institute of Petroleum Industry, Iran4- Department of Iranian offshore Oil company5- M. Sc, Marin Biology Dept, Islamic Azad University, IranAbstracts of Articles in EnglishJ.Env. Sci. Tech., Vol 17, No.1, Spring 201520415/8, 15/04, 39/22U/ml/mg protein and in aquarium 6, with 250ppb concentration, after the mentionedperiod, Catalase (CAT) activities shown respectively 15/54, 18/8, 15/81, 15/97U/ml/mg protein.Conclusion: There was no correlation between Superoxide dismutase (SOD) activities and differentconcentrations of crude oil, during the animals exposed to crude oil. It also detected that CAT enzymeis sensitive parameter than SOD and that could be useful biomarker for the evaluation of contaminatedaquatic ecosystems in Tetraclita rufotincta barnacles.
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