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      • Open Access Article

        1 - Study and comparison of phenolic compounds, flavonoids and antioxidant activity of the native populations of licorice (Glycyrrhiza glabra L.) Iran
        ghasem eghlima mohsen sanikhani Azizollah Kheiry javad hadian mitra aelaei
        Glycyrrhiza glabra L. is a family of Legominos, herbaceous, perennial and Iran is one of the countries exporting its root. This plant grows in different regions of Iran, hence the study of phytochemical diversity in different regions is important. This study was carried More
        Glycyrrhiza glabra L. is a family of Legominos, herbaceous, perennial and Iran is one of the countries exporting its root. This plant grows in different regions of Iran, hence the study of phytochemical diversity in different regions is important. This study was carried out to evaluate the content of phenolic compounds and antioxidant activity of 2 cm diameter roots of licorice populations in 15 provinces and 30 regions of Iran in 2017 in October and were transferred to the laboratories of the Department of Horticulture of Zanjan University. Total phenol (Folin–Ciocalteu method), total flavonoid (Aluminum Chloride method), anthocyanin (pH difference method) and antioxidant activity (DPPH method) were studied. The results of analysis of variance of data showed that among 30 populations, for all traits, there was a significant difference in the level of one percent. Total phenol traits ranging from 456.05 to 826 mg gallic acid per 100 g, total flavonoid between 1909.25 to 292.62 mg of quercetin per 100 g, anthocyanin levels ranging from 6.89 to 26.24 mg cyanidine-3-glucoside in Liter, the antioxidant activity varied between 62.07% and 87.14%. There was a significant and positive correlation between phenol total phenotype and antioxidant activity at 1% level, but there was no significant correlation with total flavonoid, anthocyanin and height. Based on the results of cluster analysis, 30 G. glabra were divided into two main groups. The evaluation of populations in terms of phytochemical traits showed a high variation that N, KA, BA, T, E, Y, M, MR, and SB populations Manuscript profile
      • Open Access Article

        2 - Evaluation of glabridin content and its relationship with environmental factors in some different populations of Glycyrrhiza glabra L. native to Iran
        ghasem eghlima mohsen sanikhani Azizollah Kheiry Javad Hadian mitra aelaei
        Glabridin is one of the most flavonoid compounds in Glycyrrhiza glabra L. root with biological activities such as antioxidant, antibacterial, anti-diabetic, antimicrobial, anti-cancer and neuroprotective properties. In this study, in order to investigate the content of More
        Glabridin is one of the most flavonoid compounds in Glycyrrhiza glabra L. root with biological activities such as antioxidant, antibacterial, anti-diabetic, antimicrobial, anti-cancer and neuroprotective properties. In this study, in order to investigate the content of glabridin and its relationship with soil and climatic factors, roots with a diameter of 2 cm from 17 populations of licorice were harvested from different regions of Iran in October 2018. and transferred to the Laboratory of Research Institute of Medicinal Plants and Raw Materials at Shahid Beheshti University for analysis. Extraction of the extract was performed using 80% methanol solvent and the content of glabridin was studied by HPLC. Glabridin content of different licorice populations varied from 0.41 (in Yasuj population) to 22.87 (in Kashmar population) mg / g dry weight. Significant positive correlation was observed between glabridin and phosphorus and soil lime content at 1% level. Glabridin content had a significant negative correlation with altitude of 5%. Based on the results of cluster analysis, 17 licorice populations were divided into three main groups. Evaluation of populations for glabridin showed a high diversity so that Kashmar, Ilam and Bojnurd populations can be used as superior populations for domestication and breeding and for food and pharmaceutical industries. Manuscript profile
      • Open Access Article

        3 - Effects of different drying methods on drying time and some phytochemical traits of Solidago virgaurea L.
        Sepideh Parsafar ghasem eghlima Mohammad Hossein Mirjalili Samad Nejad Ebrahimi Javad Hadian
        To investigate the effect of different drying methods on the Solidago virgaurea L., an experiment based on a completely randomized design with six treatments and three replications was conducted in 2018 in the Ecophysiology Laboratory of Shahid Beheshti University of Te More
        To investigate the effect of different drying methods on the Solidago virgaurea L., an experiment based on a completely randomized design with six treatments and three replications was conducted in 2018 in the Ecophysiology Laboratory of Shahid Beheshti University of Tehran. In this study, aerial parts of S. virgaurea L. was harvested from Sangdeh natural habitat located in Mazandan province at full flowering stage. The drying methods were shade and sun-drying (natural drying), oven-drying (40 and 50 °C), and microwave-drying (550 and 1000 watt). In all methods, drying of the plant materials was continued until the moisture content reached 10% (based on plant fresh weight). Methanolic extract was obtained from the dried samples and some secondary metabolites including content of total phenol, total flavonoid and leiocarposide were measured by Folin-Ciocalteu method, aluminum chloride method, and high-performance liquid chromatography (HPLC), respectively. The minimum (7 minutes) and maximum (60 hours) drying times were related to 1000 watt microwave drying and shade drying methods, respectively. The highest content of total phenol (30 mg Gallic acid /g DW) in shading drying treatment and the highest content of total flavonoids (7.95 mg Rutin/g DW) and leiocarposide (3.07 mg/g DW) was observed in the oven at 40 °C. In addition, active metabolites content decreased with increasing microwave power and oven temperature. In general, the correct choice of drying method depends on several factors, and among them, the type of active ingredient, optimal energy consumption and cost savings should also be considered. Manuscript profile
      • Open Access Article

        4 - Comparison content of parthenolide as an anti-migraine in Natural samples and tissue culture seedlings of some populations of Tanacetum parthenium L.
        Reyhaneh Zandi Ali sonboli ghasem eghlima Hassan Rezadoost Mohammad Hossein Mirjalili
        Sesqui-terpene lactone - parthenolide in Tanacetum parthenium L. is known as the most important medicinal substance with anti-cancer, anti-inflammatory, anti-spasm and especially anti-migraine effects. In this study, the content of parthenolide in the flowering branches More
        Sesqui-terpene lactone - parthenolide in Tanacetum parthenium L. is known as the most important medicinal substance with anti-cancer, anti-inflammatory, anti-spasm and especially anti-migraine effects. In this study, the content of parthenolide in the flowering branches of some natural populations of T. parthenium L. from different habitats in Gilan (Masoleh), Mazandaran (Chalos), Qom (Vishnu) and Tehran (Ahar) provinces were collected at the full flowering stage and compared with their flowered seedlings under in vitro conditions. In vitro seedlings were seeded and cultured on Murashige and Skoog medium enriched with hydrolyzed casein (100 mg/l), salicylic acid (2 mg/l) and benzylaminopurine (5 mg/l). The parthenolide content of flowering branches of all samples was measured using high performance liquid chromatography (HPLC). The results of variance analysis of the data showed that the effect of population and cultivation conditions as well as their interaction at the probability level of 1% on parthenolide content was significant. The parthenolide content in self-grown populations ranged from 0.13±0.007 to 1.03±0.113 mg/g dry weight and in tissue culture seedlings from 0.23±0.011 to 1.21±0.148 mg/g dry weight was variable. The highest and lowest parthenolide content was observed in Qom (Vashno) and Gilan (Masuleh) populations, respectively. Parthenolide content for all populations was higher in tissue culture samples than in natural samples. The findings of this study can be considered and exploited for the commercial production of parthenolide pharmaceutical composition under controlled conditions. Manuscript profile