Dracocephalum kotschyi is one of the most important medicinal plants of the Lamiaceae family, which is endangered due to unfavorable environmental conditions. Therefore, in order to evaluate the effectiveness of biofertilizers on changes in some physiological and bioche
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Dracocephalum kotschyi is one of the most important medicinal plants of the Lamiaceae family, which is endangered due to unfavorable environmental conditions. Therefore, in order to evaluate the effectiveness of biofertilizers on changes in some physiological and biochemical traits, the effect of biofertilizers at four levels (nitroxin, super nitroplus, biophosphorus and non-use of biofertilizer) on Dracocephalum kotschyi growth under drought stress at three levels (irrigation until the completion of 80%, 60 and 40% of field capacity), an experiment in 1398 in the form of factorial in a completely randomized design with 3 replications in a greenhouse was carried out. The study of changes in photosynthetic pigments, chlorophyll fluorescence and leaf relative water content in Dracocephalum kotschyi in response to various biofertilizers and different irrigation regimes showed that with increasing drought stress, chlorophyll a, b and total chlorophyll along with chlorophyll fluorescence (Fv/Fm) had a significant decrease. While the use of supernitroplus and biophosphorus biofertilizers in irrigation conditions up to 60 and 40% of field capacity significantly increased the above-mentioned traits in the Dracocephalum kotschyi leaves in comparison with plants treated with nitroxin fertilizer and control plants (Without biofertilizer). Increased drought intensity also caused a significant increase in carbohydrates, soluble proteins, anthocyanins and flavonoids, although the use of biofertilizers containing growth-promoting bacteria, further enhanced the amount of these compounds compared to the control (no fertilizer treatment). The highest levels of anthocyanins (37.82 mg g-1 fresh weight) and flavonoid compounds (16.62 mg g-1 fresh weight) were found in plants grown under severe drought stress (irrigation up to 40% of field capacity) and Biophosphorus application.
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