Thymus L. is an aromatic plant are belonging to the Lamiaceae family based on the synthesis of secondary metabolites widely used for medicinal purposes thymol and carvacrol. The current study aimed at evaluating the quantity and quality of essential oil composition in six species of Thymus: T. daenensis, T. kotschyanus, T. migricus, T. vulgaris, T. transcaucasicusand T. trautvetterii. The experiment was done in a randomized complete block design with three duplications in 2016 in Shahrekord University. Flowering branches in 100% flowering stage within a distance of five centimeters soil surface and water distillation (Clevenger apparatus), were analyzed by GC/MS (p< 0.05). treatments on weight and essential volume indicated. T. vulgaris was the highest volume (0.28 ml) and weight of essence (0.27 gr). The comparisons were showed that the T. trautvetterii has the highest yields (1.1 precent) and highest volume percent of oil in T.migricus and was equivalent to 1.02 ml/gr. ρ-cymene (19.46%) and γ-terpinene (14.17%) were the abundant components of T. vulgaris, thymol (60.4 %) was the highest in T. daenensis, carvacrol (6.95%) in T. kotschyanus and geraniol (41.38%) was the most in T. trautvetterii. The highest output combined was observed in T. kotschyanus (98.52%).
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