Efficacy of Sodium Bentonite, Esterified Glucomannan and Humic Acid to counteract the effects of Aflatoxicosis on renal pathological changes and serum biochemical parameters in Broilers
Subject Areas : Veterinary Clinical Pathologyحسن Ghahri 1 , A.H Soleymannezhad 2 , داریوش Mohajeri 3 , افشین Zakeri 4
1 - Department of Animal Sciences, Faculty of Animal Medicine, Islamic Azad University- Urmia Branch, Urmia, Iran
2 - Department of Animal Sciences, Faculty of Animal Medicine, Islamic Azad University- Urmia Branch, Urmia, Iran
3 - Department of Pathobiology, Faculty of Veterinary Medicine, Islamic Azad University- Tabriz Branch, Tabriz, Iran
4 - Department of Animal Sciences, Faculty of Agriculture, Islamic Azad University- Tabriz Branch, Tabriz, Iran
Keywords: Broiler, Aflatoxin, kidney, serum biochemical parameters, Adsorbents,
Abstract :
This study was conducted to evaluate the renal pathological changes and serum biochemical parameters in broilers fed a diet containing low-levels (254 ppb) of aflatoxin (AF) and adsorbents until 35 days of age. Seven-day-old male broilers were randomly assigned to one of the nine dietary treatments with four replicates of 12 chicks each. Treatments were: 1) control; 2) naturally AF contaminated diet (NCD); 3,4,5,6 and 7) NCD supplemented with 0.2%, 0.4%, 0.6%, 0.8% and 1%, humic acid (HA) respectively; 8 and 9) NCD supplemented with 0.5% sodium bentonite (SB) and 0.1% esterified glucomannan (E-GM), respectively. Blood samples were taken on the 35th day of the study and the phosphorus, calcium, urea nitrogen (BUN), and creatinine levels in serum were analyzed. When the chicks reached 35 days of age, the feeding trial was terminated and 12 broilers from each treatment were selected at random and were killed for pathological examination. Microscopically, the kidney in chickens fed diet containing 254ppb AF showed significant lesions, compared to control. At the end of the study, it was determined that aflatoxin caused a decrease in BUN and an increase in phosphorous levels. The addition of HA and E-GM to NCD decreased both the alterations of serum biochemical parameters and the severity of lesions in the kidney. These results clearly demonstrated that histological lesions and serum biochemical changes were observed in chickens fed a diet containing 254ppb AF and that addition of dietary E-GM and HA is effective in counteracting the toxic effects of naturally contaminated feed with mycotoxins.
1- Afzali, N. and Devegowda, G. 1999. Ability of modified mannanoligosaccharide to counteract aflatoxicosis in broiler breeder hens. J. Poult. Sci. 78(1):228-229.
2- Aravind, K.L., Patil, V.S., Devegowda, G., Umakantha, B. and Ganpule, S.P. 2003. Efficacy of esterified glucomannan to counteract mycotoxicosis in naturally contaminated feed on performance and serum biochemical and hematological parameters in broilers. J. Poult. Sci. 82:571-576.
3- Association of Official Analytical Chemists (AOAC). 1995. Official Methods of Analysis. 6th ed. AOAC. Washington, DC. J. Poult. Sci. 82:571-576.
4- Bailey, R.H., Kubena, L.F., Harvey, R.B., Buckley, S.A. and Rottinghaus, G.E. 1998. Efficacy of various inorganic sorbents to reduce the toxicity of aflatoxin and T-2 toxin in broiler chickens. Poult. Sci. 77:1630-1632.
5- Dafalla, R., Hassan, Y.M. and Adam, S.E. 1987. Fatty and hemorrhagic liver and kidney syndrome in breeding hens caused by aflatoxin B1 and heat stress in the Sudan. Vet. Hum. Toxicol. 29:252-254.
6- Espada, Y., Domingo, M., Gomez, J. and Calvo, M.A. 1992. Pathological lesions following an experimental intoxication with aflatoxin B1 in broiler chickens. Vet. Res. Sci. 53:275-279.
7- Glahn, R.P., Beers, K.W., Bottje, W.G., Wideman, R.F., Huff, W.E. and Thomas, W. 1991. Aflatoxicosis alters avian renal function, calcium and vitamin D metabolism. J. Toxicol Environ. Health. 34:309-321.
8- Glahn, R.P. 1993. Mycotoxins and the avian kidney. Assessment of physiological function. World Poultry Sci J. 49: 242-249.
9- Islam, K.M.S., Schuhmacher, A. and Gropp, J.M. 2005. Humic acid substances in animal agriculture. P.J.N. 4:126-134.
10- Jansen van Rensburg, C., Van Rensburg, C.E.J., Van Ryssen, J.B.J., Casey, N.H. and Rottinghaus, N.H. 2006. In vitro and in vivo assessment of humic acid as an aflatoxin binder in broiler chickens. Poult. Sci. 85:1576-1583.
11- Kececi, T., Oguz, H., Kurtoglu, V. and Demet, O. 1998. Effect of polyvinylpolypyrrolidone, synthetic zeolite and bentonite on serum biochemical and hematological characters of broiler chickens during aflatoxicosis. Brit Poultry Sci. 39: 452-458.
12- Kokhan, E., Dine, E., Mehmet, A., Fatma, S. and Levent, A. 2005. Effect of dietary aflatoxin and sodium bentonite on some hormones in broiler chickens. Bull. Vet. Lnst Pulawy. 49:93-96.
13- National Research Council. 1994. Nutrient Requirements of Poultry. 9th rev. ed. Natl. Acad. Press, Washington, DC.
14- Ramos, A.J., Fink- Gremmels, J. and Hernandez, E. 1996. Prevention of toxic effects of mycotoxins by means of nonnutritive adsorbent compounds. J Food Protect. 59(6):631-641.
15- Romer, T.R. 1975. Screening method for the detection of aflatoxins in mixed feed and other agricultural commodities with subsequent confirmation and quantitative measurement of aflatoxins in positive samples. J Assoc Off Ana Chem. 58: 500-506.
16- SAS Institute. 2004. SAS(r) User’s Guide. Statistics. Version 9.1 ed. SAS Institute Inc. Cary. NC.
17- Whitlow, L.W. and Hagler, W.M. 2002. Mycotoxins in feeds. Feedstuffs. 74:10-11.
18- Younus, M. 1994. Mycosis and Mycotoxicosis. Poultry International. Oct. p:24-27.
